##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063872_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2320939
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.225722003034118	32.0	32.0	32.0	32.0	32.0
2	30.77544175008477	32.0	32.0	32.0	32.0	32.0
3	30.805414101792422	32.0	32.0	32.0	32.0	32.0
4	30.844313443825968	32.0	32.0	32.0	32.0	32.0
5	30.724862652572945	32.0	32.0	32.0	32.0	32.0
6	34.38975647356523	36.0	36.0	36.0	32.0	36.0
7	34.32971008716731	36.0	36.0	36.0	32.0	36.0
8	34.28547238854619	36.0	36.0	36.0	32.0	36.0
9	34.43817825457713	36.0	36.0	36.0	32.0	36.0
10	34.09759713633146	36.0	36.0	36.0	32.0	36.0
11	34.418607727303474	36.0	36.0	36.0	32.0	36.0
12	34.21534559934578	36.0	36.0	36.0	32.0	36.0
13	34.30422471249783	36.0	36.0	36.0	32.0	36.0
14	34.201709739032346	36.0	36.0	36.0	32.0	36.0
15	34.13385099737649	36.0	36.0	36.0	32.0	36.0
16	34.1302899386843	36.0	36.0	36.0	32.0	36.0
17	34.06624172371613	36.0	36.0	36.0	32.0	36.0
18	34.05746725786417	36.0	36.0	36.0	32.0	36.0
19	34.067980244202886	36.0	36.0	36.0	32.0	36.0
20	34.035324926678385	36.0	36.0	36.0	32.0	36.0
21	34.01776220745138	36.0	36.0	36.0	32.0	36.0
22	34.00344343388603	36.0	36.0	36.0	32.0	36.0
23	33.951482137186716	36.0	36.0	36.0	32.0	36.0
24	33.928407855613614	36.0	36.0	36.0	32.0	36.0
25	33.51269421557396	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	4.0
4	11.0
5	25.0
6	88.0
7	51.0
8	133.0
9	148.0
10	195.0
11	76.0
12	144.0
13	140.0
14	222.0
15	390.0
16	645.0
17	846.0
18	1164.0
19	1626.0
20	2433.0
21	3712.0
22	5927.0
23	9059.0
24	13337.0
25	19750.0
26	27632.0
27	36480.0
28	49059.0
29	65728.0
30	85910.0
31	115958.0
32	163062.0
33	228709.0
34	481189.0
35	1007085.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.8163241026985	17.371450148192466	11.6634539620579	26.148771787051135
2	15.899161879860685	19.76724295056552	38.83842951685828	25.495165652715517
3	18.77781243860151	24.437115882432018	28.491872132582824	28.29319954638365
4	12.401345539764904	16.016654221529755	36.11193815377363	35.470062084931705
5	14.224601326892342	37.14656528612813	33.88539362734896	14.743439759630569
6	33.56202074001297	35.65519782300965	17.531365337504607	13.251416099472774
7	29.98814442341862	30.47499447300001	21.12701670856269	18.409844395018677
8	27.73324965993314	34.00840986633519	19.317133680325085	18.941206793406582
9	27.637927548782816	14.376614077884389	18.57878812305939	39.40667025027341
10	15.853342570410799	27.14627331776481	31.951809701259293	25.0485744105651
11	36.337911712479915	20.854509109265926	23.641412941749067	19.16616623650509
12	25.335852876607913	23.248736348162595	29.56824582188674	21.847164953342748
13	29.758031764043462	20.17820388897847	25.251112534803088	24.81265181217498
14	23.416464099736224	20.223394173833036	25.524640445494907	30.83550128093583
15	24.67448483630905	28.537770623145658	22.232291207706307	24.555453332838987
16	24.909675539014994	25.76506327454842	25.01189954712738	24.313361639309207
17	23.626144322856625	26.351825010109962	25.788943623048823	24.233087043984593
18	24.059404702512953	25.195255316295718	27.338935115255936	23.40640486593539
19	25.69280295549935	24.684395753366893	25.385800805621933	24.237000485511828
20	25.83205122013481	24.77616200851369	25.35759746935002	24.034189302001476
21	26.231760263644794	24.332081023430256	24.635377747575546	24.800780965349407
22	25.731168900048253	25.186395409655955	24.870112874273058	24.212322816022738
23	24.08164268539632	25.163214651630504	25.618059604179678	25.137083058793497
24	24.918019356271383	25.01430484079262	25.73431156740954	24.333364235526457
25	24.566093759365135	25.052962196224666	25.773929361603408	24.60701468280679
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	212.0
1	212.0
2	519.0
3	826.0
4	826.0
5	826.0
6	1989.5
7	3153.0
8	3153.0
9	3153.0
10	3367.5
11	3582.0
12	3582.0
13	3582.0
14	4487.0
15	5392.0
16	5392.0
17	5392.0
18	9485.0
19	13578.0
20	13578.0
21	13578.0
22	23817.0
23	34056.0
24	34056.0
25	34056.0
26	53223.5
27	72391.0
28	72391.0
29	72391.0
30	95381.0
31	118371.0
32	118371.0
33	118371.0
34	140641.0
35	162911.0
36	162911.0
37	162911.0
38	182139.5
39	201368.0
40	201368.0
41	201368.0
42	227263.0
43	253158.0
44	253158.0
45	253158.0
46	279324.0
47	305490.0
48	305490.0
49	305490.0
50	307880.0
51	310270.0
52	310270.0
53	310270.0
54	286893.0
55	263516.0
56	263516.0
57	263516.0
58	239788.0
59	216060.0
60	216060.0
61	216060.0
62	187213.5
63	158367.0
64	158367.0
65	158367.0
66	128583.0
67	98799.0
68	98799.0
69	98799.0
70	74293.5
71	49788.0
72	49788.0
73	49788.0
74	37253.0
75	24718.0
76	24718.0
77	24718.0
78	19365.0
79	14012.0
80	14012.0
81	14012.0
82	9598.5
83	5185.0
84	5185.0
85	5185.0
86	3969.5
87	2754.0
88	2754.0
89	2754.0
90	2032.5
91	1311.0
92	1311.0
93	1311.0
94	926.5
95	542.0
96	542.0
97	542.0
98	835.5
99	1129.0
100	1129.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04235354742197016
2	0.006462901437737054
3	0.0015080103354719791
4	0.0037053968243025773
5	0.007669309706114637
6	0.013098146913813764
7	0.02206003690747581
8	0.03477040973502535
9	0.04808398669676368
10	0.056097984479557626
11	0.05463306015367056
12	0.06337952009940803
13	0.0629486600035589
14	0.066395540770352
15	0.05967412327510547
16	0.06561999259782354
17	0.06225928385020029
18	0.07738247321450499
19	0.07484039864899508
20	0.07975220374167524
21	0.07574520485027826
22	0.07798567734869379
23	0.08216502027843041
24	0.07729630119533516
25	0.07893356955956188
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2320939.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.908575536088485
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.24430144314294	37.054273266992226
2	14.396855891335234	14.3705313946977
3	4.863092462051679	7.281300524438662
4	2.1513470369150336	4.294826643848563
5	1.1601025492335877	2.8949532854016664
6	0.7032310318210478	2.1058355442577326
7	0.4691944646668754	1.6391779166658946
8	0.32119294306707297	1.2824225808577263
9	0.24470142463901826	1.0991429581846415
>10	1.2635230072648866	11.279651121780535
>50	0.09632327981360707	3.3314297845969074
>100	0.07314206094844518	7.46783806559307
>500	0.009527763740325755	3.1465399875449926
>1k	0.0034646413601183406	2.7520769251397423
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4670	0.20121166476154695	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3259	0.14041730523723372	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2920	0.12581114798794799	No Hit
GTCCTACAGTGGACATTTCTAAATT	2384	0.10271704685043426	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2382	0.10263087483126442	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2325	0.10017497228492432	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.3086009584913694E-5
2	0.0	0.0	0.0	0.0	4.3086009584913694E-5
3	0.0	0.0	0.0	0.0	4.3086009584913694E-5
4	0.0	0.0	0.0	0.0	4.3086009584913694E-5
5	0.0	0.0	0.0	0.0	4.3086009584913694E-5
6	0.0	0.0	0.0	0.0	4.3086009584913694E-5
7	0.0	0.0	0.0	0.0	4.3086009584913694E-5
8	0.0	0.0	0.0	0.0	4.3086009584913694E-5
9	0.0	0.0	0.0	0.0	4.3086009584913694E-5
10	0.0	0.0	0.0	0.0	4.3086009584913694E-5
11	0.0	0.0	0.0	0.0	4.3086009584913694E-5
12	0.0	0.0	0.0	0.0	3.877740862642232E-4
13	0.0	0.0	0.0	0.0	4.3086009584913693E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAG	345	0.0	12.663088	1
GCGTTAT	135	3.7289283E-10	11.959583	1
GAACCGA	80	2.8710178E-5	11.873693	6
GTATCAA	3360	0.0	11.702051	1
CGTGCGG	65	8.024734E-4	11.6925335	10
GTCCTAG	270	0.0	11.607831	1
ACCGTCG	320	0.0	11.578599	8
CGTCGTA	335	0.0	11.343502	10
ATACCGT	345	0.0	11.288612	6
CGAGCCG	330	0.0	11.229669	15
GTCCTAT	475	0.0	11.196835	1
GCCTAGT	85	5.3405987E-5	11.173312	1
CGATAAC	120	1.2758392E-7	11.083548	10
CCGTCGT	345	0.0	11.014942	9
TTAACGA	190	0.0	11.000924	12
TTAGGAC	935	0.0	10.96898	3
GTATTAT	175	2.0008883E-11	10.854075	1
CGTTATT	150	2.1500455E-9	10.761769	2
GCTCGTA	115	8.8170964E-7	10.739569	9
TACCGTC	345	0.0	10.738643	7
>>END_MODULE