FastQCFastQC Report
Thu 2 Feb 2017
SRR4063872_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063872_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2320939
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTAA752.0665466E-623.46547341
GTATCAA42300.021.6873071
TAGGACG17000.020.0578024
TTAGGAC11800.019.574433
GACGTGA10150.019.5059767
GTCCTAC18050.018.7695311
TAGGACC14850.018.2212944
GTAGGAC21200.017.9511413
TCCTACA20150.017.7948532
AGGACGT18550.017.7888535
GGTATCA23150.017.7705361
GGACGTG17850.017.623756
ACGATTA1002.4861565E-517.59872640
CTGTAGG21400.017.3733221
TGTAGGA21550.017.2512782
TTTAGGA15700.016.5334762
AACGCAG55500.016.4496146
GATATAC6450.016.0305371
CCTACAG21450.015.9984963
CTTTAGG15150.015.8279171