##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063872_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2320939
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.324553553540184	32.0	32.0	32.0	32.0	32.0
2	31.387270410812175	32.0	32.0	32.0	32.0	32.0
3	31.473837528689895	32.0	32.0	32.0	32.0	32.0
4	31.561508940993278	32.0	32.0	32.0	32.0	32.0
5	31.509891901510553	32.0	32.0	32.0	32.0	32.0
6	35.09715895161398	36.0	36.0	36.0	36.0	36.0
7	35.11576435227294	36.0	36.0	36.0	36.0	36.0
8	35.06424813405264	36.0	36.0	36.0	36.0	36.0
9	35.17023325473009	36.0	36.0	36.0	36.0	36.0
10	35.02995985676487	36.0	36.0	36.0	36.0	36.0
11	35.1731902475679	36.0	36.0	36.0	36.0	36.0
12	35.085621810827426	36.0	36.0	36.0	36.0	36.0
13	35.12035689003459	36.0	36.0	36.0	36.0	36.0
14	35.06769630739972	36.0	36.0	36.0	36.0	36.0
15	35.03864901231786	36.0	36.0	36.0	36.0	36.0
16	35.053213806997945	36.0	36.0	36.0	36.0	36.0
17	35.02343275717285	36.0	36.0	36.0	36.0	36.0
18	35.01764673694569	36.0	36.0	36.0	36.0	36.0
19	35.01315803646714	36.0	36.0	36.0	36.0	36.0
20	34.99921023344431	36.0	36.0	36.0	36.0	36.0
21	34.99764448785599	36.0	36.0	36.0	36.0	36.0
22	34.98214515762801	36.0	36.0	36.0	36.0	36.0
23	34.930909429330114	36.0	36.0	36.0	36.0	36.0
24	34.908186298735124	36.0	36.0	36.0	32.0	36.0
25	34.876559444259414	36.0	36.0	36.0	32.0	36.0
26	34.823274545345654	36.0	36.0	36.0	32.0	36.0
27	34.808260794445694	36.0	36.0	36.0	32.0	36.0
28	34.780636630260425	36.0	36.0	36.0	32.0	36.0
29	34.757277119303865	36.0	36.0	36.0	32.0	36.0
30	34.73280168069906	36.0	36.0	36.0	32.0	36.0
31	34.72230894478485	36.0	36.0	36.0	32.0	36.0
32	34.682563393523054	36.0	36.0	36.0	32.0	36.0
33	34.65953176709944	36.0	36.0	36.0	32.0	36.0
34	34.65435282874733	36.0	36.0	36.0	32.0	36.0
35	34.61863625024182	36.0	36.0	36.0	32.0	36.0
36	34.5870938443449	36.0	36.0	36.0	32.0	36.0
37	34.56518762449164	36.0	36.0	36.0	32.0	36.0
38	34.52828574986245	36.0	36.0	36.0	32.0	36.0
39	34.499552982650556	36.0	36.0	36.0	32.0	36.0
40	34.49288369922691	36.0	36.0	36.0	32.0	36.0
41	34.47137904098298	36.0	36.0	36.0	32.0	36.0
42	34.421836162001675	36.0	36.0	36.0	32.0	36.0
43	34.41839272811565	36.0	36.0	36.0	32.0	36.0
44	34.343222721493326	36.0	36.0	36.0	32.0	36.0
45	34.31062298492119	36.0	36.0	36.0	32.0	36.0
46	34.27219931243346	36.0	36.0	36.0	32.0	36.0
47	34.25688008172554	36.0	36.0	36.0	32.0	36.0
48	34.20064465287541	36.0	36.0	36.0	32.0	36.0
49	34.17939463294813	36.0	36.0	36.0	32.0	36.0
50	33.60222952865198	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	7.0
19	5.0
20	30.0
21	61.0
22	237.0
23	720.0
24	1824.0
25	4276.0
26	8784.0
27	15723.0
28	26191.0
29	40436.0
30	58510.0
31	83128.0
32	121043.0
33	195668.0
34	406123.0
35	1358172.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.06445483008334	18.2560543554043	12.259267143126763	26.42022367138559
2	15.338771672894778	20.524712302702927	38.365542418574634	25.770973605827667
3	17.93783259878488	25.119962842433218	28.847094286033126	28.095110272748773
4	12.124833957290562	16.680490094741828	36.37170128124867	34.82297466671894
5	13.921434384962295	37.50344149501559	33.66361632080809	14.911507799214025
6	33.623862796674615	35.96179987806638	17.315176857602648	13.099160467656354
7	29.859897222632735	30.593393449806307	21.190216545975574	18.356492781585384
8	27.649110984821228	34.16608536458735	19.200030677238825	18.984772973352595
9	27.62687173592048	14.197725285847302	18.477991578292453	39.69741139993977
10	15.755877631178697	27.32552377981471	31.9868689889087	24.931729600097892
11	36.56214144361399	20.779520702612174	23.602516050615723	19.055821803158118
12	25.33622240011995	23.30018863095633	29.51346311441859	21.850125854505126
13	29.91959236924604	20.09367820747254	25.21353564356265	24.773193779718767
14	23.3858639546527	20.137169033819173	25.492388402589995	30.984578608938136
15	24.656018496775086	28.585161301720024	22.169329427271283	24.589490774233607
16	24.936350698709695	25.768821946115356	24.96194398689141	24.33288336828354
17	23.595363773024623	26.452138552542742	25.684302775729996	24.268194898702635
18	24.129067171751338	25.13392255090737	27.313004996701757	23.424005280639538
19	25.78128938330564	24.628910970947533	25.5354836986237	24.054315947123126
20	26.001628664495115	24.67211278286197	25.331161780673185	23.995096771969738
21	26.261612218158252	24.25867289058437	24.638303721037047	24.84141117022033
22	25.779082232340745	25.121287273755073	24.937051258630138	24.162579235274045
23	24.117999860400065	25.002951418361185	25.753818101285788	25.125230619952966
24	25.027348915285362	24.955179662661525	25.750635627999603	24.266835794053513
25	24.761784118998715	24.85213703827698	25.884797227281325	24.501281615442984
26	24.051790293176424	25.750370235360677	26.16767945481362	24.03016001664928
27	24.70072845507717	25.494376512946822	25.327796167014675	24.477098864961334
28	24.12971615596341	25.312169841075605	25.656267612066603	24.901846390894377
29	24.49478345769676	24.996660758003625	25.33429043908232	25.1742653452173
30	24.227539497161185	25.127334099427667	25.856502516520795	24.788623886890353
31	25.158482019745186	24.756158306215873	25.14960564778556	24.935754026253377
32	24.561510849178344	25.489827723863538	25.09506145763631	24.85359996932181
33	23.994846693438582	25.306871649919856	25.82905327381465	24.869228382826908
34	24.725480928114674	25.240243235638943	25.96863168534998	24.065644150896407
35	24.969526658195747	25.235201632483527	25.796066235192583	23.999205474128143
36	24.174983227556705	25.503377972234226	25.681680786255946	24.639958013953123
37	24.797747735326155	25.41682752630055	25.032703338067204	24.752721400306093
38	24.593214803094508	25.75908388552361	25.145534346967676	24.50216696441421
39	25.250749944632066	24.840424019255114	25.11105902264613	24.797767013466686
40	25.663517012870063	25.02600295576266	25.514091576938156	23.79638845442912
41	24.805029506252843	25.016373212734567	25.94835371654694	24.23024356446565
42	25.263238625738833	25.913633429818976	25.356306050502862	23.46682189393933
43	24.374168430341175	25.380195371474663	25.71252162942843	24.53311456875573
44	24.141643891923845	25.562288041935112	25.314239747272527	24.98182831886852
45	24.24825919799665	25.722248098054518	25.29832010075345	24.731172603195382
46	24.425636752354023	25.081110146933238	25.288312641808115	25.204940458904623
47	25.16733889470802	24.995680534484848	25.39660449077918	24.440376080027953
48	24.934507939188897	25.671121275785346	24.762375845149194	24.631994939876563
49	24.74260384603453	25.505442631765806	24.987720471396024	24.76423305080364
50	24.442696085063336	26.243544546827835	24.67613940418095	24.63761996392788
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	21.0
1	29.5
2	38.0
3	117.5
4	197.0
5	225.0
6	253.0
7	270.5
8	288.0
9	367.5
10	447.0
11	689.5
12	932.0
13	1833.0
14	2734.0
15	3718.5
16	4703.0
17	5097.5
18	5492.0
19	5570.5
20	5649.0
21	6327.0
22	7005.0
23	7947.5
24	8890.0
25	10720.0
26	12550.0
27	16956.0
28	21362.0
29	27078.0
30	32794.0
31	39626.5
32	46459.0
33	56100.0
34	65741.0
35	77619.5
36	89498.0
37	97387.5
38	105277.0
39	108383.5
40	111490.0
41	116744.0
42	121998.0
43	123643.5
44	125289.0
45	136125.0
46	146961.0
47	155195.5
48	163430.0
49	168701.0
50	173972.0
51	171267.0
52	168562.0
53	166102.5
54	163643.0
55	163112.0
56	162581.0
57	157376.0
58	152171.0
59	138085.0
60	123999.0
61	108482.5
62	92966.0
63	79652.5
64	66339.0
65	55898.0
66	45457.0
67	38395.0
68	31333.0
69	27728.5
70	24124.0
71	18632.5
72	13141.0
73	11095.0
74	9049.0
75	6973.5
76	4898.0
77	4203.5
78	3509.0
79	2955.0
80	2401.0
81	1901.5
82	1402.0
83	1166.5
84	931.0
85	683.0
86	435.0
87	312.0
88	189.0
89	152.0
90	115.0
91	88.5
92	62.0
93	59.0
94	56.0
95	54.0
96	52.0
97	42.5
98	33.0
99	27.0
100	21.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009177320041586617
2	4.308600958491369E-4
3	5.170321150189644E-4
4	0.0
5	0.0
6	1.7234403833965478E-4
7	0.0
8	0.0
9	0.0013787523067172382
10	9.048062012831875E-4
11	0.0
12	2.1543004792456844E-4
13	6.462901437737054E-4
14	3.0160206709439584E-4
15	0.004394772977661197
16	7.755481725284465E-4
17	0.0
18	3.4468807667930955E-4
19	0.0
20	8.186341821133602E-4
21	0.0
22	1.292580287547411E-4
23	9.048062012831875E-4
24	2.585160575094822E-4
25	0.0017234403833965475
26	0.005084149131019815
27	0.0059027833131331765
28	0.003748482833887491
29	0.0022835585080004258
30	0.003274536728453441
31	0.0073677076390202415
32	0.003102192690113786
33	0.004265514948906456
34	0.002671332594264649
35	0.002498988555924994
36	0.0072384496102655
37	0.003231450718868527
38	0.007798567734869378
39	0.004351686968076283
40	0.002111214469660771
41	0.003231450718868527
42	0.0017665263929814612
43	0.0011633222587926696
44	8.617201916982737E-4
45	0.0011202362492077561
46	9.048062012831875E-4
47	0.002154300479245685
48	0.0020250424504909435
49	1.292580287547411E-4
50	0.001421838316302152
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2320939.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.690967753670385
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.8654466941142	40.19598284317815
2	14.292579225147668	15.347648205883655
3	4.8224761696012575	7.76770237544715
4	2.0803273166531473	4.467791475020151
5	1.0884527529561536	2.92200408301813
6	0.6617123553735988	2.1316786040741507
7	0.4444861821139225	1.6705425289581473
8	0.29609504907945566	1.2718103785717203
9	0.2212434033271891	1.069089519037716
>10	1.0853294907623041	10.356382046651984
>50	0.07707512676014865	2.8932117795970425
>100	0.055977973161367454	6.086277688893742
>500	0.0067803111904552425	2.432360413682576
>1k	0.0020179497590636923	1.3875180579857516
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.617201916982739E-5
2	0.0	0.0	0.0	0.0	1.292580287547411E-4
3	0.0	0.0	0.0	0.0	1.292580287547411E-4
4	0.0	0.0	0.0	0.0	1.292580287547411E-4
5	0.0	0.0	0.0	0.0	1.292580287547411E-4
6	0.0	0.0	0.0	0.0	1.292580287547411E-4
7	0.0	0.0	0.0	0.0	1.292580287547411E-4
8	0.0	0.0	0.0	0.0	1.292580287547411E-4
9	0.0	0.0	0.0	0.0	1.292580287547411E-4
10	0.0	0.0	0.0	0.0	1.292580287547411E-4
11	0.0	0.0	0.0	0.0	1.292580287547411E-4
12	0.0	0.0	0.0	0.0	2.585160575094822E-4
13	0.0	0.0	0.0	0.0	3.0160206709439584E-4
14	0.0	0.0	0.0	0.0	3.0160206709439584E-4
15	0.0	0.0	0.0	0.0	3.4468807667930955E-4
16	0.0	0.0	0.0	0.0	3.4468807667930955E-4
17	0.0	0.0	0.0	0.0	4.3086009584913693E-4
18	0.0	0.0	0.0	4.3086009584913694E-5	4.3086009584913693E-4
19	0.0	0.0	0.0	1.292580287547411E-4	4.3086009584913693E-4
20	0.0	0.0	0.0	2.1543004792456846E-4	6.032041341887917E-4
21	0.0	0.0	0.0	2.585160575094822E-4	6.893761533586191E-4
22	0.0	0.0	0.0	3.4468807667930955E-4	6.893761533586191E-4
23	0.0	0.0	0.0	7.755481725284464E-4	6.893761533586191E-4
24	0.0	0.0	0.0	0.0011633222587926696	6.893761533586191E-4
25	0.0	0.0	0.0	0.001421838316302152	6.893761533586191E-4
26	0.0	0.0	0.0	0.0018957844217362024	8.617201916982739E-4
27	0.0	0.0	0.0	0.002369730527170253	8.617201916982739E-4
28	0.0	0.0	0.0	0.0031452786996986996	8.617201916982739E-4
29	0.0	0.0	0.0	0.005170321150189643	8.617201916982739E-4
30	0.0	0.0	0.0	0.008617201916982738	8.617201916982739E-4
31	0.0	0.0	0.0	0.01770834993939953	9.478922108681012E-4
32	0.0	0.0	0.0	0.02843676632604304	9.90978220453015E-4
33	0.0	0.0	0.0	0.039122096703101635	9.90978220453015E-4
34	0.0	0.0	0.0	0.05153086746355678	0.0010771502396228422
35	0.0	0.0	0.0	0.06665405682786148	0.0010771502396228422
36	0.0	0.0	0.0	0.08694756734235583	0.0010771502396228422
37	0.0	0.0	0.0	0.1192620745310411	0.0010771502396228422
38	0.0	0.0	0.0	0.16480398666229487	0.0010771502396228422
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATTAA	75	2.0665466E-6	23.465473	41
GTATCAA	4230	0.0	21.687307	1
TAGGACG	1700	0.0	20.057802	4
TTAGGAC	1180	0.0	19.57443	3
GACGTGA	1015	0.0	19.505976	7
GTCCTAC	1805	0.0	18.769531	1
TAGGACC	1485	0.0	18.221294	4
GTAGGAC	2120	0.0	17.951141	3
TCCTACA	2015	0.0	17.794853	2
AGGACGT	1855	0.0	17.788853	5
GGTATCA	2315	0.0	17.770536	1
GGACGTG	1785	0.0	17.62375	6
ACGATTA	100	2.4861565E-5	17.598726	40
CTGTAGG	2140	0.0	17.373322	1
TGTAGGA	2155	0.0	17.251278	2
TTTAGGA	1570	0.0	16.533476	2
AACGCAG	5550	0.0	16.449614	6
GATATAC	645	0.0	16.030537	1
CCTACAG	2145	0.0	15.998496	3
CTTTAGG	1515	0.0	15.827917	1
>>END_MODULE