FastQCFastQC Report
Thu 2 Feb 2017
SRR4063871_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063871_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8632
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGG921.065801668211307No Hit
GGATAACCTTGCACTGAAGATCCCC250.2896200185356812No Hit
TTGCTATAGTGACCATCCCTTTCTC250.2896200185356812No Hit
GGCATGTACTCTTCATCTTCTTTTT240.27803521779425394No Hit
CCCATGAATAATCCTGTTTTGATGG230.26645041705282674No Hit
AGGTTATCCTGCTCGGAGAGCCAGG190.2201112140871177No Hit
CATAAATGCAGGCATTTTCCAGAAC190.2201112140871177No Hit
CTATAAATGCATTGAAAACTCAAAC170.19694161260426318No Hit
CTATGGGTCAGGTTCGGGCTCCGGC170.19694161260426318No Hit
GTCTCAGGATAAAGAGTTTTACAGA170.19694161260426318No Hit
GTCAAATACATAGGAAAAATAAAAC150.17377201112140872No Hit
GTTGTGAATGTACTACGCTTGATCT150.17377201112140872No Hit
ATTCATGGGAGGGCCGATGTTATTG150.17377201112140872No Hit
GGCTTGTCCTGGCTCTCGCCTTCGT140.16218721037998146No Hit
GGTGAGAGGCCTTTCGCCTGCAGCT140.16218721037998146No Hit
GTGAGAATCCTGTCAAAAGGCTTAT140.16218721037998146No Hit
GTCTAGAAGAATGAGCAGGGACAGG140.16218721037998146No Hit
GTACAAACTCGTGCGTGCAGGCACA140.16218721037998146No Hit
GTCCAGGCGCGGGTGCTCAGGGTGA140.16218721037998146No Hit
GGATATCTACAGGGAAATGGGTTTT130.15060240963855423No Hit
GTATCAACGCAGAGTACATGGGGAA130.15060240963855423No Hit
CTATAGCAACAGCCACAAACCCAAG130.15060240963855423No Hit
CTTTAGGTATGAAAGATTGTTCATC130.15060240963855423No Hit
GTACATGGGGGGCTGCGAGAAAGTT130.15060240963855423No Hit
GGATAAACCAGCCAATTTGGTTCTG130.15060240963855423No Hit
GGGTATGTGGTGAGGATCTGAACTC130.15060240963855423No Hit
GGATTCTCACTGAGCAAAACCAAGA120.13901760889712697No Hit
GTGCAAGGTTATCCTGCTCGGAGAG120.13901760889712697No Hit
ATATTGCTTTCATCTGTTGGCTGGT120.13901760889712697No Hit
AGTTTGTACAGTATGTGGAGGCAAG120.13901760889712697No Hit
GGTATTCCCATTCCATGTCACCTAG120.13901760889712697No Hit
ACCTTGCACTGAAGATCCCCAAACC120.13901760889712697No Hit
TCCTGAGACTGGTCATTTAAACGTG120.13901760889712697No Hit
GCATTAAAGCATAATTCCTTACGAG120.13901760889712697No Hit
GGATGCAGGTTCCCGTCGGCAGCAG110.1274328081556997No Hit
GTATCAACGCAGAGTACTTTTTTTT110.1274328081556997No Hit
GCTTTCATCTGTTGGCTGGTATTCC110.1274328081556997No Hit
TTATTATACAATAACTTTCAAAGCC110.1274328081556997No Hit
GGTTTATCCTGTTCCTTTTCATACT110.1274328081556997No Hit
ATCTTATACTAGATGAGCAGGGAGA110.1274328081556997No Hit
TAATGGGGGTGTGTGTTAATTTCTT110.1274328081556997No Hit
GTATCAACGCAGAGTACATGGGTAC110.1274328081556997No Hit
GTCCTGGCTCTCGCCTTCGTCCTGG110.1274328081556997No Hit
CTGTGGTCCCTTCTCCTCGATGCAG100.11584800741427247No Hit
GTGTACCACGTTTAAATGACCAGTC100.11584800741427247No Hit
GGGTGAGCTGGCCGGGCTGATGGTG100.11584800741427247No Hit
GCCTGGAGCTTCCCCCGCTCCTGTC100.11584800741427247No Hit
GTCTTCTTCCTTTTCAAATTCAATA100.11584800741427247No Hit
GCCCCACACCATCAGTGTAACCTGA100.11584800741427247No Hit
GTATAATAATGGGGGTGTGTGTTAA100.11584800741427247No Hit
GTATAGGGGTCCTAGGAAGATAATA100.11584800741427247No Hit
GTACTGGATTATTAGTCGCATGCAC100.11584800741427247No Hit
GACTTGGAGATAATATTCTCAAGAT100.11584800741427247No Hit
ATCCAGTACTGGGGAGACAGGAGCG100.11584800741427247No Hit
TTTCTAGGGAGATGGCTCAGTGGGT100.11584800741427247No Hit
ATCTACAGGGAAATGGGTTTTGGTC100.11584800741427247No Hit
GAATAATCCTGTTTTGATGGAAGGA100.11584800741427247No Hit
CTACAGGGAAATGGGTTTTGGTCAT100.11584800741427247No Hit
ATGTTATTGGATTCATCTATTAGGT90.10426320667284524No Hit
GCCAGGTACCAGTGGGTCCGCTGCA90.10426320667284524No Hit
CCCCTATACTATATCAATTTACCAG90.10426320667284524No Hit
GTACATGCCAATCAAAAATCCTAAT90.10426320667284524No Hit
CAATAACATCGGCCCTCCCATGAAT90.10426320667284524No Hit
TATTAAACCTCCAGAAAATGTGCTG90.10426320667284524No Hit
GAGCAGACCTGCTACAATTGTCCAC90.10426320667284524No Hit
GTCAAACTGTGGTCCCTTCTCCTCG90.10426320667284524No Hit
TCTCAAGTTCGCTCAGCTTTCCTAT90.10426320667284524No Hit
TCTCACAGATGGGGCAGCTGAACTT90.10426320667284524No Hit
GTATACTTAGTGTACCACGTTTAAA90.10426320667284524No Hit
GTATAAGATCATATTTTGCATCCTG90.10426320667284524No Hit
GGTCAGGTTCGGGCTCCGGCTCTGG90.10426320667284524No Hit
GCTCCGGCCTGAGCTGGGGCACGCA90.10426320667284524No Hit
GGTCTGCTCCGGCCTGAGCTGGGGC90.10426320667284524No Hit
AAACAGGATTATTCATGGGAGGGCC90.10426320667284524No Hit
CTTCCAGCCTGTCCCTGCTCATTCT90.10426320667284524No Hit
TCTTTGGACTCCTGCTTCTGTCCCT90.10426320667284524No Hit
ATATGATCTTATACTAGATGAGCAG90.10426320667284524No Hit
AGCCAGGACAAGCCTGCTGCCGACG90.10426320667284524No Hit
TATCAACGCAGAGTACTTTTTTTTT90.10426320667284524No Hit
GGTAACAGCATTAAAGCATAATTCC90.10426320667284524No Hit
CCACATACCCAGTGCATGCGACTAA90.10426320667284524No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)