##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063871_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8632
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.33433734939759	32.0	32.0	32.0	32.0	32.0
2	31.06371640407785	32.0	32.0	32.0	32.0	32.0
3	30.977293790546803	32.0	32.0	32.0	32.0	32.0
4	31.086075069508805	32.0	32.0	32.0	32.0	32.0
5	30.989573679332715	32.0	32.0	32.0	32.0	32.0
6	34.68964318813716	36.0	36.0	36.0	32.0	36.0
7	34.60055607043559	36.0	36.0	36.0	32.0	36.0
8	34.65199258572753	36.0	36.0	36.0	32.0	36.0
9	34.640176088971266	36.0	36.0	36.0	32.0	36.0
10	34.45956904541242	36.0	36.0	36.0	32.0	36.0
11	34.696362372567194	36.0	36.0	36.0	32.0	36.0
12	34.533016682113065	36.0	36.0	36.0	32.0	36.0
13	34.60055607043559	36.0	36.0	36.0	32.0	36.0
14	34.56522242817424	36.0	36.0	36.0	32.0	36.0
15	34.49443929564411	36.0	36.0	36.0	32.0	36.0
16	34.480885078776645	36.0	36.0	36.0	32.0	36.0
17	34.43385078776645	36.0	36.0	36.0	32.0	36.0
18	34.45203892493049	36.0	36.0	36.0	32.0	36.0
19	34.44821594068582	36.0	36.0	36.0	32.0	36.0
20	34.43431417979611	36.0	36.0	36.0	32.0	36.0
21	34.35403151065802	36.0	36.0	36.0	32.0	36.0
22	34.35461075069509	36.0	36.0	36.0	32.0	36.0
23	34.39828544949027	36.0	36.0	36.0	32.0	36.0
24	34.34765987025023	36.0	36.0	36.0	32.0	36.0
25	33.95157553290083	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	2.0
15	1.0
16	1.0
17	4.0
18	3.0
19	9.0
20	5.0
21	9.0
22	10.0
23	29.0
24	30.0
25	58.0
26	78.0
27	110.0
28	151.0
29	205.0
30	252.0
31	331.0
32	540.0
33	729.0
34	1639.0
35	4435.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.868799258229025	19.17014371812703	12.227630968938339	24.73342605470561
2	18.721037998146432	21.33920296570899	38.0097312326228	21.930027803521778
3	17.75949953660797	27.23586654309546	29.645505097312324	25.359128822984246
4	14.006024096385541	18.17655236329935	37.34939759036144	30.46802594995366
5	15.74374420759963	35.86654309545876	33.433734939759034	14.955977757182575
6	30.827154772937902	36.7354031510658	17.794253938832252	14.64318813716404
7	28.591288229842448	29.518072289156628	24.77988878591288	17.110750695088043
8	23.965697068026422	32.95862788272106	23.166067910534245	19.909607138718275
9	26.808066759388037	15.391747797867408	19.552619378766806	38.24756606397774
10	15.969405493104647	26.770193533433773	34.78966276509445	22.470738208367134
11	33.383545770567785	23.835457705677868	25.133256083429895	17.64774044032445
12	23.041261010662957	25.54473806212332	31.571627260083453	19.84237366713027
13	27.491886879925826	23.446917014371813	26.7964765878535	22.264719517848864
14	22.464356091341138	21.247247015184882	27.738495421351573	28.549901472122407
15	24.82327036736586	28.751883184610037	23.25877853748986	23.166067910534245
16	25.0	26.692165044042653	26.36764024107557	21.94019471488178
17	22.76573548162745	28.75854874232062	26.18523240987597	22.29048336617596
18	22.42058891722699	27.15047530721076	28.680732668676097	21.74820310688616
19	25.397009389127156	26.428654225107223	26.579343920250377	21.594992465515244
20	25.133317876188265	26.26941803848829	27.02295386042198	21.57431022490146
21	23.716239712530427	26.22000695490901	26.927089370580738	23.13666396197983
22	23.127753303964756	27.625782517968933	27.486668212381172	21.75979596568514
23	24.240667748666823	26.93021099003014	27.16206816600974	21.667053095293298
24	22.705146036161334	28.31478905887807	26.35605006954103	22.624014835419565
25	25.976585139677756	26.046134229743828	26.022951199721806	21.95432943085661
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	1.0
4	1.0
5	1.0
6	4.0
7	7.0
8	7.0
9	7.0
10	11.0
11	15.0
12	15.0
13	15.0
14	22.0
15	29.0
16	29.0
17	29.0
18	63.5
19	98.0
20	98.0
21	98.0
22	165.5
23	233.0
24	233.0
25	233.0
26	369.5
27	506.0
28	506.0
29	506.0
30	604.5
31	703.0
32	703.0
33	703.0
34	810.5
35	918.0
36	918.0
37	918.0
38	927.5
39	937.0
40	937.0
41	937.0
42	971.0
43	1005.0
44	1005.0
45	1005.0
46	1016.0
47	1027.0
48	1027.0
49	1027.0
50	923.5
51	820.0
52	820.0
53	820.0
54	699.0
55	578.0
56	578.0
57	578.0
58	547.0
59	516.0
60	516.0
61	516.0
62	454.5
63	393.0
64	393.0
65	393.0
66	373.5
67	354.0
68	354.0
69	354.0
70	275.0
71	196.0
72	196.0
73	196.0
74	156.5
75	117.0
76	117.0
77	117.0
78	78.5
79	40.0
80	40.0
81	40.0
82	35.5
83	31.0
84	31.0
85	31.0
86	18.5
87	6.0
88	6.0
89	6.0
90	4.5
91	3.0
92	3.0
93	3.0
94	3.0
95	3.0
96	3.0
97	3.0
98	49.5
99	96.0
100	96.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04633920296570899
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.03475440222428174
9	0.04633920296570899
10	0.03475440222428174
11	0.023169601482854494
12	0.04633920296570899
13	0.04633920296570899
14	0.05792400370713623
15	0.03475440222428174
16	0.04633920296570899
17	0.05792400370713623
18	0.06950880444856349
19	0.05792400370713623
20	0.06950880444856349
21	0.05792400370713623
22	0.06950880444856349
23	0.06950880444856349
24	0.04633920296570899
25	0.05792400370713623
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	8632.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.71130676552364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.57413183614057	39.87488415199258
2	12.788521522145976	14.249304911955516
3	5.635267207319608	9.418443002780352
4	3.451861093782491	7.6923076923076925
5	1.9338739862757333	5.38693234476367
6	1.1436889166146809	3.822984244670992
7	1.039717196922437	4.054680259499537
8	0.7485963817841547	3.3364226135310475
9	0.47826991058432106	2.3980537534754403
>10	1.1852776044915783	8.700185356811863
>50	0.020794343938448742	1.065801668211307
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGG	92	1.065801668211307	No Hit
GGATAACCTTGCACTGAAGATCCCC	25	0.2896200185356812	No Hit
TTGCTATAGTGACCATCCCTTTCTC	25	0.2896200185356812	No Hit
GGCATGTACTCTTCATCTTCTTTTT	24	0.27803521779425394	No Hit
CCCATGAATAATCCTGTTTTGATGG	23	0.26645041705282674	No Hit
AGGTTATCCTGCTCGGAGAGCCAGG	19	0.2201112140871177	No Hit
CATAAATGCAGGCATTTTCCAGAAC	19	0.2201112140871177	No Hit
CTATAAATGCATTGAAAACTCAAAC	17	0.19694161260426318	No Hit
CTATGGGTCAGGTTCGGGCTCCGGC	17	0.19694161260426318	No Hit
GTCTCAGGATAAAGAGTTTTACAGA	17	0.19694161260426318	No Hit
GTCAAATACATAGGAAAAATAAAAC	15	0.17377201112140872	No Hit
GTTGTGAATGTACTACGCTTGATCT	15	0.17377201112140872	No Hit
ATTCATGGGAGGGCCGATGTTATTG	15	0.17377201112140872	No Hit
GGCTTGTCCTGGCTCTCGCCTTCGT	14	0.16218721037998146	No Hit
GGTGAGAGGCCTTTCGCCTGCAGCT	14	0.16218721037998146	No Hit
GTGAGAATCCTGTCAAAAGGCTTAT	14	0.16218721037998146	No Hit
GTCTAGAAGAATGAGCAGGGACAGG	14	0.16218721037998146	No Hit
GTACAAACTCGTGCGTGCAGGCACA	14	0.16218721037998146	No Hit
GTCCAGGCGCGGGTGCTCAGGGTGA	14	0.16218721037998146	No Hit
GGATATCTACAGGGAAATGGGTTTT	13	0.15060240963855423	No Hit
GTATCAACGCAGAGTACATGGGGAA	13	0.15060240963855423	No Hit
CTATAGCAACAGCCACAAACCCAAG	13	0.15060240963855423	No Hit
CTTTAGGTATGAAAGATTGTTCATC	13	0.15060240963855423	No Hit
GTACATGGGGGGCTGCGAGAAAGTT	13	0.15060240963855423	No Hit
GGATAAACCAGCCAATTTGGTTCTG	13	0.15060240963855423	No Hit
GGGTATGTGGTGAGGATCTGAACTC	13	0.15060240963855423	No Hit
GGATTCTCACTGAGCAAAACCAAGA	12	0.13901760889712697	No Hit
GTGCAAGGTTATCCTGCTCGGAGAG	12	0.13901760889712697	No Hit
ATATTGCTTTCATCTGTTGGCTGGT	12	0.13901760889712697	No Hit
AGTTTGTACAGTATGTGGAGGCAAG	12	0.13901760889712697	No Hit
GGTATTCCCATTCCATGTCACCTAG	12	0.13901760889712697	No Hit
ACCTTGCACTGAAGATCCCCAAACC	12	0.13901760889712697	No Hit
TCCTGAGACTGGTCATTTAAACGTG	12	0.13901760889712697	No Hit
GCATTAAAGCATAATTCCTTACGAG	12	0.13901760889712697	No Hit
GGATGCAGGTTCCCGTCGGCAGCAG	11	0.1274328081556997	No Hit
GTATCAACGCAGAGTACTTTTTTTT	11	0.1274328081556997	No Hit
GCTTTCATCTGTTGGCTGGTATTCC	11	0.1274328081556997	No Hit
TTATTATACAATAACTTTCAAAGCC	11	0.1274328081556997	No Hit
GGTTTATCCTGTTCCTTTTCATACT	11	0.1274328081556997	No Hit
ATCTTATACTAGATGAGCAGGGAGA	11	0.1274328081556997	No Hit
TAATGGGGGTGTGTGTTAATTTCTT	11	0.1274328081556997	No Hit
GTATCAACGCAGAGTACATGGGTAC	11	0.1274328081556997	No Hit
GTCCTGGCTCTCGCCTTCGTCCTGG	11	0.1274328081556997	No Hit
CTGTGGTCCCTTCTCCTCGATGCAG	10	0.11584800741427247	No Hit
GTGTACCACGTTTAAATGACCAGTC	10	0.11584800741427247	No Hit
GGGTGAGCTGGCCGGGCTGATGGTG	10	0.11584800741427247	No Hit
GCCTGGAGCTTCCCCCGCTCCTGTC	10	0.11584800741427247	No Hit
GTCTTCTTCCTTTTCAAATTCAATA	10	0.11584800741427247	No Hit
GCCCCACACCATCAGTGTAACCTGA	10	0.11584800741427247	No Hit
GTATAATAATGGGGGTGTGTGTTAA	10	0.11584800741427247	No Hit
GTATAGGGGTCCTAGGAAGATAATA	10	0.11584800741427247	No Hit
GTACTGGATTATTAGTCGCATGCAC	10	0.11584800741427247	No Hit
GACTTGGAGATAATATTCTCAAGAT	10	0.11584800741427247	No Hit
ATCCAGTACTGGGGAGACAGGAGCG	10	0.11584800741427247	No Hit
TTTCTAGGGAGATGGCTCAGTGGGT	10	0.11584800741427247	No Hit
ATCTACAGGGAAATGGGTTTTGGTC	10	0.11584800741427247	No Hit
GAATAATCCTGTTTTGATGGAAGGA	10	0.11584800741427247	No Hit
CTACAGGGAAATGGGTTTTGGTCAT	10	0.11584800741427247	No Hit
ATGTTATTGGATTCATCTATTAGGT	9	0.10426320667284524	No Hit
GCCAGGTACCAGTGGGTCCGCTGCA	9	0.10426320667284524	No Hit
CCCCTATACTATATCAATTTACCAG	9	0.10426320667284524	No Hit
GTACATGCCAATCAAAAATCCTAAT	9	0.10426320667284524	No Hit
CAATAACATCGGCCCTCCCATGAAT	9	0.10426320667284524	No Hit
TATTAAACCTCCAGAAAATGTGCTG	9	0.10426320667284524	No Hit
GAGCAGACCTGCTACAATTGTCCAC	9	0.10426320667284524	No Hit
GTCAAACTGTGGTCCCTTCTCCTCG	9	0.10426320667284524	No Hit
TCTCAAGTTCGCTCAGCTTTCCTAT	9	0.10426320667284524	No Hit
TCTCACAGATGGGGCAGCTGAACTT	9	0.10426320667284524	No Hit
GTATACTTAGTGTACCACGTTTAAA	9	0.10426320667284524	No Hit
GTATAAGATCATATTTTGCATCCTG	9	0.10426320667284524	No Hit
GGTCAGGTTCGGGCTCCGGCTCTGG	9	0.10426320667284524	No Hit
GCTCCGGCCTGAGCTGGGGCACGCA	9	0.10426320667284524	No Hit
GGTCTGCTCCGGCCTGAGCTGGGGC	9	0.10426320667284524	No Hit
AAACAGGATTATTCATGGGAGGGCC	9	0.10426320667284524	No Hit
CTTCCAGCCTGTCCCTGCTCATTCT	9	0.10426320667284524	No Hit
TCTTTGGACTCCTGCTTCTGTCCCT	9	0.10426320667284524	No Hit
ATATGATCTTATACTAGATGAGCAG	9	0.10426320667284524	No Hit
AGCCAGGACAAGCCTGCTGCCGACG	9	0.10426320667284524	No Hit
TATCAACGCAGAGTACTTTTTTTTT	9	0.10426320667284524	No Hit
GGTAACAGCATTAAAGCATAATTCC	9	0.10426320667284524	No Hit
CCACATACCCAGTGCATGCGACTAA	9	0.10426320667284524	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE