FastQCFastQC Report
Thu 2 Feb 2017
SRR4063871_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063871_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8632
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCTATAGTGACCATCCCTTTCTCGAGACCGGCTACGACTGCGGTTCTG360.41705282669138094No Hit
ATTCATGGGAGGGCCGATGTTATTGGATTCATCTATTAGGTCAAACTGTG240.27803521779425394No Hit
TGCTAATCCAGAGGCTGAGTGGAGCCGAGCTGGTCAGGATGCAGGTTCCC230.26645041705282674No Hit
ATCTTATACTAGATGAGCAGGGAGAAGACTCCAAATCAAGTCACTCACAC210.24328081556997222No Hit
CCACATACCCAGTGCATGCGACTAATAATCCAGTACTGGGGAGACAGGAG190.2201112140871177No Hit
CCCATGAATAATCCTGTTTTGATGGAAGGACCCTCAAAAGATTTCATCTC180.20852641334569047No Hit
GTACATGGGGGGCTGCGAGAAAGTTTACGGGAAATCTTCGCACCTCAAGG170.19694161260426318No Hit
GTATACTTAGTGTACCACGTTTAAATGACCAGTCTCAGGATAAAGAGTTT160.18535681186283595No Hit
TCCAGAGGCTGAGTGGAGCCGAGCTGGTCAGGATGCAGGTTCCCGTCGGC160.18535681186283595No Hit
GTCTCAGGATAAAGAGTTTTACAGAAAATTTAAAATGCCTGGAAAAGACT150.17377201112140872No Hit
GGTGAGAGGCCTTTCGCCTGCAGCTGGCAGGAGTGCAACAAGAAGTTCGC150.17377201112140872No Hit
AGTTTGTACAGTATGTGGAGGCAAGAGGTCAGTGCCGAGTGTCTTCCTCG150.17377201112140872No Hit
CTATAGCAACAGCCACAAACCCAAGTACCAGACAGAACCTTATGAGAAGG150.17377201112140872No Hit
GTATAATAATGGGGGTGTGTGTTAATTTCTTAAATGTTGATTCAATACAC150.17377201112140872No Hit
AGGTTATCCTGCTCGGAGAGCCAGGTACCAGTGGGTCCGCTGCAAACCGA140.16218721037998146No Hit
CTTCTAGACCATGTTTTAGGTTCATCTGTGTGAGCAACACCTACCGTTCT140.16218721037998146No Hit
GTCAAATACATAGGAAAAATAAAACGGTTTCTTATCATTTGGAATCAAGG140.16218721037998146No Hit
CTTTTAGGTTGTTTGTTAGTTTTTGCTATCTGAGGAATGCCACTGTGAAC130.15060240963855423No Hit
GTATCAACGCAGAGTACATGGGTACATTTGCTAATCCAGAGGCTGAGTGG130.15060240963855423No Hit
GTACCAGTGGGTCCGCTGCAAACCGAATGGCTTTTTTGCGAACTGCATCG130.15060240963855423No Hit
CATAAATGCAGGCATTTTCCAGAACAACAAGATAGGTAACAGCATTAAAG130.15060240963855423No Hit
TAATAAAATCGTCTTCTGGTTGGTCTTGGTTTTGCTCAGTGAGAATCCTG120.13901760889712697No Hit
GTAGATATCCTGATTAGGATTTTTGATTGGCATGTACTCTTCATCTTCTT120.13901760889712697No Hit
GTATTCGGGTGTGTGTGTGCCTGCACGCACGAGTTTGTACAGTATGTGGA120.13901760889712697No Hit
ACGCAGAGTACATGGGTACATTTGCTAATCCAGAGGCTGAGTGGAGCCGA120.13901760889712697No Hit
GGGTATGTGGTGAGGATCTGAACTCAGTCCTGTGCTGATGAGGAAACACT120.13901760889712697No Hit
GTGAGAATCCTGTCAAAAGGCTTATAGTTGAAATAGACAATATTGCTTTC120.13901760889712697No Hit
GAATACATTGTTCCACATGGAGGTGGGGAGACAGGGACCGTCACATTCAT120.13901760889712697No Hit
GGCTACGACTGCGGTTCTGACGTCGGTCTCTCTTTTCATTCTTCTGTTTC120.13901760889712697No Hit
CGCCTGGACCGCGCCTCCTCACCCCTTTGGTAATAGGAAAGCTGAGCGAA110.1274328081556997No Hit
GTACAAACTCGTGCGTGCAGGCACACACACACCCGAATACACAGCTGTTC110.1274328081556997No Hit
GTCCTGGCTCTCGCCTTCGTCCTGGTTTGGGGATCTTCAGTGCAAGGTTA110.1274328081556997No Hit
GTCTAGAAGAATGAGCAGGGACAGGCTGGAAGCAACGAGAAGTCATCCAC110.1274328081556997No Hit
CTATGGGTCAGGTTCGGGCTCCGGCTCTGGCTCCGGCTCTGGCTCGGGTT110.1274328081556997No Hit
GGATAACCTTGCACTGAAGATCCCCAAACCAGGACGAAGGCGAGAGCCAG100.11584800741427247No Hit
GTATCAACGCAGAGTACATGGGGAAGATCAGCTGAAGAAGTAGAAGAAAT100.11584800741427247No Hit
CTATTAGGTCAAACTGTGGTCCCTTCTCCTCGATGCAGTTCGCAAAAAAG100.11584800741427247No Hit
GTGCAAGGTTATCCTGCTCGGAGAGCCAGGTACCAGTGGGTCCGCTGCAA100.11584800741427247No Hit
GGATAAACCAGCCAATTTGGTTCTGAAAGAAAAAGTAAAGCCCAAACAGG100.11584800741427247No Hit
CATTAGTAGAACGCAAAATCTTAGGGTACATACAACTACGAAAAGGCCCT100.11584800741427247No Hit
CCCCTGACCCCATACAATTCACATACCAGGTAAATTACAGCAACCATAAC100.11584800741427247No Hit
GTATGTGGAGGCAAGAGGTCAGTGCCGAGTGTCTTCCTCGGTCACTCGAA100.11584800741427247No Hit
TCCACATACTGTACAAACTCGTGCGTGCAGGCACACACACACCCGAATAC100.11584800741427247No Hit
GGTCAGGATGCAGGTTCCCGTCGGCAGCAGGCTTGTCCTGGCTCTCGCCT100.11584800741427247No Hit
AGGTACCAGTGGGTCCGCTGCAAACCGAATGGCTTTTTTGCGAACTGCAT100.11584800741427247No Hit
TTTCTAGGGAGATGGCTCAGTGGGTACAGTGTTTCCTCATCAGCACAGGA100.11584800741427247No Hit
ACCTAAAACATGGTCTAGAAGAATGAGCAGGGACAGGCTGGAAGCAACGA100.11584800741427247No Hit
GTCAAAAGGCTTATAGTTGAAATAGACAATATTGCTTTCATCTGTTGGCT90.10426320667284524No Hit
TTATTGGATTCATCTATTAGGTCAAACTGTGGTCCCTTCTCCTCGATGCA90.10426320667284524No Hit
GTCAAACTGTGGTCCCTTCTCCTCGATGCAGTTCGCAAAAAAGCCATTCG90.10426320667284524No Hit
GTATAAGATCATATTTTGCATCCTGTTTGGGCTTTACTTTTTCTTTCAGA90.10426320667284524No Hit
GTACATGGGTACATTTGCTAATCCAGAGGCTGAGTGGAGCCGAGCTGGTC90.10426320667284524No Hit
GTCTGGTACTTGGGTTTGTGGCTGTTGCTATAGTGACCATCCCTTTCTCG90.10426320667284524No Hit
GCTCCAGGCAGTTCGAGTGACCGAGGAAGACACTCGGCACTGACCTCTTG90.10426320667284524No Hit
CTATAAATGCATTGAAAACTCAAACCACATGGCCAGAAAGTGCCATGCTC90.10426320667284524No Hit
GGTCAGGTTCGGGCTCCGGCTCTGGCTCCGGCTCTGGCTCGGGTTCCGGC90.10426320667284524No Hit
GAAGAGTACATGCCAATCAAAAATCCTAATCAGGATATCTACAGGGAAAT90.10426320667284524No Hit
TATCAACGCAGAGTACATGGGTACATTTGCTAATCCAGAGGCTGAGTGGA90.10426320667284524No Hit
GTCCAGGCGCGGGTGCTCAGGGTGAGCTGGCCGGGCTGATGGTGGGGCTG90.10426320667284524No Hit
GGATATCTACAGGGAAATGGGTTTTGGTCATTATGAAGAAGAAGAAAGCT90.10426320667284524No Hit
GTACTACGCTTGATCTGTTGATGGACTTTTAGGTTGTTTGTTAGTTTTTG90.10426320667284524No Hit
TTCCCATTCCATGTCACCTAGGAAGCCACTTCCGGAGCCGGAACCCGAGC90.10426320667284524No Hit
TCCTAACACTCCTCGTCCCCATTCTAATCGCCATAGCCTTCCTAACATTA90.10426320667284524No Hit
GGCTTATAGTTGAAATAGACAATATTGCTTTCATCTGTTGGCTGGTATTC90.10426320667284524No Hit
TAATGGGGGTGTGTGTTAATTTCTTAAATGTTGATTCAATACACATAAAT90.10426320667284524No Hit
GGATGCAGGTTCCCGTCGGCAGCAGGCTTGTCCTGGCTCTCGCCTTCGTC90.10426320667284524No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers