##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063871_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8632
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29483317886932	32.0	32.0	32.0	32.0	32.0
2	31.453776645041707	32.0	32.0	32.0	32.0	32.0
3	31.537303058387394	32.0	32.0	32.0	32.0	32.0
4	31.541589434661724	32.0	32.0	32.0	32.0	32.0
5	31.56510658016682	32.0	32.0	32.0	32.0	32.0
6	35.15616311399444	36.0	36.0	36.0	36.0	36.0
7	35.165894346617236	36.0	36.0	36.0	36.0	36.0
8	35.10403151065802	36.0	36.0	36.0	36.0	36.0
9	35.143535681186286	36.0	36.0	36.0	36.0	36.0
10	34.9943234476367	36.0	36.0	36.0	36.0	36.0
11	35.179680259499534	36.0	36.0	36.0	36.0	36.0
12	35.127664504170525	36.0	36.0	36.0	36.0	36.0
13	35.15593141797961	36.0	36.0	36.0	36.0	36.0
14	35.06962465245598	36.0	36.0	36.0	36.0	36.0
15	35.04321130676552	36.0	36.0	36.0	36.0	36.0
16	35.15859592215014	36.0	36.0	36.0	36.0	36.0
17	35.01436515291937	36.0	36.0	36.0	36.0	36.0
18	35.04796107506951	36.0	36.0	36.0	36.0	36.0
19	35.05954587581093	36.0	36.0	36.0	36.0	36.0
20	35.002316960148285	36.0	36.0	36.0	36.0	36.0
21	35.12140871177016	36.0	36.0	36.0	36.0	36.0
22	35.03718721037998	36.0	36.0	36.0	36.0	36.0
23	35.074721964782206	36.0	36.0	36.0	36.0	36.0
24	34.90384615384615	36.0	36.0	36.0	36.0	36.0
25	34.980537534754404	36.0	36.0	36.0	36.0	36.0
26	34.933155699721965	36.0	36.0	36.0	36.0	36.0
27	34.812673772011124	36.0	36.0	36.0	32.0	36.0
28	34.97358665430954	36.0	36.0	36.0	36.0	36.0
29	34.86631139944393	36.0	36.0	36.0	32.0	36.0
30	34.86943929564411	36.0	36.0	36.0	36.0	36.0
31	34.7645968489342	36.0	36.0	36.0	32.0	36.0
32	34.67991195551436	36.0	36.0	36.0	32.0	36.0
33	34.73853104726599	36.0	36.0	36.0	32.0	36.0
34	34.791126042632065	36.0	36.0	36.0	32.0	36.0
35	34.799698795180724	36.0	36.0	36.0	32.0	36.0
36	34.666705282669135	36.0	36.0	36.0	32.0	36.0
37	34.58677015755329	36.0	36.0	36.0	32.0	36.0
38	34.61179332715477	36.0	36.0	36.0	32.0	36.0
39	34.618744207599626	36.0	36.0	36.0	32.0	36.0
40	34.4983781278962	36.0	36.0	36.0	32.0	36.0
41	34.50011584800741	36.0	36.0	36.0	32.0	36.0
42	34.48748841519926	36.0	36.0	36.0	32.0	36.0
43	34.49038461538461	36.0	36.0	36.0	32.0	36.0
44	34.34893419833179	36.0	36.0	36.0	32.0	36.0
45	34.390407784986095	36.0	36.0	36.0	32.0	36.0
46	34.36075069508804	36.0	36.0	36.0	32.0	36.0
47	34.38727988878591	36.0	36.0	36.0	32.0	36.0
48	34.37152455977757	36.0	36.0	36.0	32.0	36.0
49	34.31383225208526	36.0	36.0	36.0	32.0	36.0
50	33.773748841519925	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	2.0
23	9.0
24	13.0
25	26.0
26	39.0
27	60.0
28	92.0
29	146.0
30	205.0
31	267.0
32	420.0
33	596.0
34	1305.0
35	5451.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.61631139944393	21.17701575532901	13.067655236329937	25.13901760889713
2	17.2613531047266	22.022706209453197	38.50787766450417	22.208063021316033
3	17.42354031510658	27.61816496756256	29.19369786839666	25.764596848934197
4	12.140871177015756	19.624652455977756	36.53846153846153	31.69601482854495
5	14.330398517145504	38.54263206672846	32.81974050046339	14.307228915662652
6	28.081556997219646	38.76274328081557	18.616774791473585	14.538924930491195
7	27.27062094531974	29.6339202965709	24.768303985171457	18.327154772937906
8	24.930491195551436	33.94346617238184	22.439759036144576	18.686283595922152
9	26.149924690070677	15.560190012744757	20.692851349785656	37.59703394739891
10	15.708989805375348	27.652919369786837	34.16357738646895	22.47451343836886
11	33.79286376274328	24.061631139944392	24.50185356811863	17.6436515291937
12	23.158016682113068	27.351714550509733	29.738183503243743	19.752085264133456
13	27.838276181649675	22.09221501390176	27.838276181649675	22.23123262279889
14	21.941612604263206	20.563021316033364	27.10843373493976	30.386932344763668
15	23.540315106580167	28.39434661723818	23.378127896200183	24.687210379981465
16	23.215940685820204	27.38646895273401	26.506024096385545	22.89156626506024
17	22.23123262279889	28.96200185356812	26.135310472659874	22.671455050973126
18	22.034291010194625	27.07367933271548	28.683966635773867	22.208063021316033
19	24.965245597775716	26.424930491195553	25.97312326227989	22.636700648748842
20	24.77988878591288	24.675625579240037	27.838276181649675	22.706209453197403
21	23.88785912882298	26.992585727525487	25.567655236329934	23.551899907321594
22	22.13855421686747	27.826691380908247	26.737720111214085	23.297034291010192
23	23.586654309545878	26.923076923076923	27.47914735866543	22.01112140871177
24	23.042168674698797	27.8498609823911	26.413345690454126	22.69462465245598
25	25.602409638554217	26.18164967562558	24.45551436515292	23.760426320667285
26	23.169601482854496	27.421223354958297	26.64504170528267	22.764133456904542
27	22.752548656163114	29.62233549582947	26.575532900834105	21.04958294717331
28	21.5129749768304	26.679796107506952	28.70713623725672	23.100092678405932
29	23.43605189990732	27.050509731232626	25.938368860055604	23.57506950880445
30	24.015291936978684	24.988415199258572	27.027340129749767	23.968952734012973
31	23.95736793327155	25.46339202965709	27.166357738646894	23.412882298424467
32	23.181186283595924	28.151065801668214	25.393883225208526	23.27386468952734
33	22.486098239110287	27.594995366079704	27.23586654309546	22.68303985171455
34	22.717794253938834	26.031047265987024	28.4870250231696	22.764133456904542
35	24.304911955514367	25.556070435588506	27.652919369786837	22.486098239110287
36	21.999536607970345	27.94253938832252	27.085264133456903	22.97265987025023
37	23.702502316960146	28.035217794253942	25.590824837812793	22.671455050973126
38	22.09221501390176	27.0389249304912	27.606580166821132	23.262279888785915
39	22.822057460611678	28.533364226135312	25.185356811862835	23.459221501390175
40	24.467099165894346	26.64504170528267	27.47914735866543	21.408711770157556
41	24.687210379981465	25.695088044485637	27.803521779425395	21.814179796107506
42	23.227525486561632	26.923076923076923	27.259036144578314	22.590361445783135
43	22.289156626506024	25.81093605189991	26.795644114921224	25.104263206672844
44	21.698331788693235	27.363299351251158	26.35542168674699	24.582947173308618
45	22.21964782205746	27.06209453197405	26.1468952734013	24.571362372567194
46	22.23123262279889	25.729842446709917	28.811399443929563	23.227525486561632
47	22.77571825764597	26.934661723818348	27.398053753475438	22.89156626506024
48	21.582483781278963	26.193234476367007	27.177942539388322	25.04633920296571
49	22.289156626506024	27.293790546802594	27.652919369786837	22.764133456904542
50	23.264975089792607	25.72123740006952	27.96894913683235	23.044838373305527
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	0.5
6	0.0
7	0.5
8	1.0
9	1.5
10	2.0
11	2.0
12	2.0
13	3.5
14	5.0
15	6.0
16	7.0
17	8.0
18	9.0
19	20.5
20	32.0
21	34.5
22	37.0
23	38.0
24	39.0
25	65.5
26	92.0
27	147.0
28	202.0
29	204.5
30	207.0
31	231.0
32	255.0
33	324.0
34	393.0
35	519.5
36	646.0
37	646.0
38	646.0
39	598.0
40	550.0
41	557.5
42	565.0
43	567.5
44	570.0
45	605.0
46	640.0
47	550.0
48	460.0
49	457.0
50	454.0
51	464.5
52	475.0
53	453.5
54	432.0
55	376.0
56	320.0
57	304.0
58	288.0
59	308.5
60	329.0
61	261.5
62	194.0
63	181.5
64	169.0
65	157.0
66	145.0
67	141.0
68	137.0
69	119.0
70	101.0
71	82.0
72	63.0
73	66.5
74	70.0
75	58.0
76	46.0
77	36.5
78	27.0
79	18.0
80	9.0
81	7.5
82	6.0
83	4.0
84	2.0
85	2.0
86	2.0
87	1.5
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.011584800741427247
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.011584800741427247
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	8632.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.777571825764596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.72093023255813	44.06858202038925
2	12.422480620155039	14.851714550509731
3	5.3100775193798455	9.522706209453197
4	3.3333333333333335	7.970342910101946
5	1.6472868217054266	4.92354031510658
6	1.0465116279069768	3.7534754402224286
7	0.6976744186046512	2.9193697868396664
8	0.5426356589147286	2.5949953660797034
9	0.3682170542635659	1.9810009267840594
>10	0.9108527131782945	7.414272474513438
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTGCTATAGTGACCATCCCTTTCTCGAGACCGGCTACGACTGCGGTTCTG	36	0.41705282669138094	No Hit
ATTCATGGGAGGGCCGATGTTATTGGATTCATCTATTAGGTCAAACTGTG	24	0.27803521779425394	No Hit
TGCTAATCCAGAGGCTGAGTGGAGCCGAGCTGGTCAGGATGCAGGTTCCC	23	0.26645041705282674	No Hit
ATCTTATACTAGATGAGCAGGGAGAAGACTCCAAATCAAGTCACTCACAC	21	0.24328081556997222	No Hit
CCACATACCCAGTGCATGCGACTAATAATCCAGTACTGGGGAGACAGGAG	19	0.2201112140871177	No Hit
CCCATGAATAATCCTGTTTTGATGGAAGGACCCTCAAAAGATTTCATCTC	18	0.20852641334569047	No Hit
GTACATGGGGGGCTGCGAGAAAGTTTACGGGAAATCTTCGCACCTCAAGG	17	0.19694161260426318	No Hit
GTATACTTAGTGTACCACGTTTAAATGACCAGTCTCAGGATAAAGAGTTT	16	0.18535681186283595	No Hit
TCCAGAGGCTGAGTGGAGCCGAGCTGGTCAGGATGCAGGTTCCCGTCGGC	16	0.18535681186283595	No Hit
GTCTCAGGATAAAGAGTTTTACAGAAAATTTAAAATGCCTGGAAAAGACT	15	0.17377201112140872	No Hit
GGTGAGAGGCCTTTCGCCTGCAGCTGGCAGGAGTGCAACAAGAAGTTCGC	15	0.17377201112140872	No Hit
AGTTTGTACAGTATGTGGAGGCAAGAGGTCAGTGCCGAGTGTCTTCCTCG	15	0.17377201112140872	No Hit
CTATAGCAACAGCCACAAACCCAAGTACCAGACAGAACCTTATGAGAAGG	15	0.17377201112140872	No Hit
GTATAATAATGGGGGTGTGTGTTAATTTCTTAAATGTTGATTCAATACAC	15	0.17377201112140872	No Hit
AGGTTATCCTGCTCGGAGAGCCAGGTACCAGTGGGTCCGCTGCAAACCGA	14	0.16218721037998146	No Hit
CTTCTAGACCATGTTTTAGGTTCATCTGTGTGAGCAACACCTACCGTTCT	14	0.16218721037998146	No Hit
GTCAAATACATAGGAAAAATAAAACGGTTTCTTATCATTTGGAATCAAGG	14	0.16218721037998146	No Hit
CTTTTAGGTTGTTTGTTAGTTTTTGCTATCTGAGGAATGCCACTGTGAAC	13	0.15060240963855423	No Hit
GTATCAACGCAGAGTACATGGGTACATTTGCTAATCCAGAGGCTGAGTGG	13	0.15060240963855423	No Hit
GTACCAGTGGGTCCGCTGCAAACCGAATGGCTTTTTTGCGAACTGCATCG	13	0.15060240963855423	No Hit
CATAAATGCAGGCATTTTCCAGAACAACAAGATAGGTAACAGCATTAAAG	13	0.15060240963855423	No Hit
TAATAAAATCGTCTTCTGGTTGGTCTTGGTTTTGCTCAGTGAGAATCCTG	12	0.13901760889712697	No Hit
GTAGATATCCTGATTAGGATTTTTGATTGGCATGTACTCTTCATCTTCTT	12	0.13901760889712697	No Hit
GTATTCGGGTGTGTGTGTGCCTGCACGCACGAGTTTGTACAGTATGTGGA	12	0.13901760889712697	No Hit
ACGCAGAGTACATGGGTACATTTGCTAATCCAGAGGCTGAGTGGAGCCGA	12	0.13901760889712697	No Hit
GGGTATGTGGTGAGGATCTGAACTCAGTCCTGTGCTGATGAGGAAACACT	12	0.13901760889712697	No Hit
GTGAGAATCCTGTCAAAAGGCTTATAGTTGAAATAGACAATATTGCTTTC	12	0.13901760889712697	No Hit
GAATACATTGTTCCACATGGAGGTGGGGAGACAGGGACCGTCACATTCAT	12	0.13901760889712697	No Hit
GGCTACGACTGCGGTTCTGACGTCGGTCTCTCTTTTCATTCTTCTGTTTC	12	0.13901760889712697	No Hit
CGCCTGGACCGCGCCTCCTCACCCCTTTGGTAATAGGAAAGCTGAGCGAA	11	0.1274328081556997	No Hit
GTACAAACTCGTGCGTGCAGGCACACACACACCCGAATACACAGCTGTTC	11	0.1274328081556997	No Hit
GTCCTGGCTCTCGCCTTCGTCCTGGTTTGGGGATCTTCAGTGCAAGGTTA	11	0.1274328081556997	No Hit
GTCTAGAAGAATGAGCAGGGACAGGCTGGAAGCAACGAGAAGTCATCCAC	11	0.1274328081556997	No Hit
CTATGGGTCAGGTTCGGGCTCCGGCTCTGGCTCCGGCTCTGGCTCGGGTT	11	0.1274328081556997	No Hit
GGATAACCTTGCACTGAAGATCCCCAAACCAGGACGAAGGCGAGAGCCAG	10	0.11584800741427247	No Hit
GTATCAACGCAGAGTACATGGGGAAGATCAGCTGAAGAAGTAGAAGAAAT	10	0.11584800741427247	No Hit
CTATTAGGTCAAACTGTGGTCCCTTCTCCTCGATGCAGTTCGCAAAAAAG	10	0.11584800741427247	No Hit
GTGCAAGGTTATCCTGCTCGGAGAGCCAGGTACCAGTGGGTCCGCTGCAA	10	0.11584800741427247	No Hit
GGATAAACCAGCCAATTTGGTTCTGAAAGAAAAAGTAAAGCCCAAACAGG	10	0.11584800741427247	No Hit
CATTAGTAGAACGCAAAATCTTAGGGTACATACAACTACGAAAAGGCCCT	10	0.11584800741427247	No Hit
CCCCTGACCCCATACAATTCACATACCAGGTAAATTACAGCAACCATAAC	10	0.11584800741427247	No Hit
GTATGTGGAGGCAAGAGGTCAGTGCCGAGTGTCTTCCTCGGTCACTCGAA	10	0.11584800741427247	No Hit
TCCACATACTGTACAAACTCGTGCGTGCAGGCACACACACACCCGAATAC	10	0.11584800741427247	No Hit
GGTCAGGATGCAGGTTCCCGTCGGCAGCAGGCTTGTCCTGGCTCTCGCCT	10	0.11584800741427247	No Hit
AGGTACCAGTGGGTCCGCTGCAAACCGAATGGCTTTTTTGCGAACTGCAT	10	0.11584800741427247	No Hit
TTTCTAGGGAGATGGCTCAGTGGGTACAGTGTTTCCTCATCAGCACAGGA	10	0.11584800741427247	No Hit
ACCTAAAACATGGTCTAGAAGAATGAGCAGGGACAGGCTGGAAGCAACGA	10	0.11584800741427247	No Hit
GTCAAAAGGCTTATAGTTGAAATAGACAATATTGCTTTCATCTGTTGGCT	9	0.10426320667284524	No Hit
TTATTGGATTCATCTATTAGGTCAAACTGTGGTCCCTTCTCCTCGATGCA	9	0.10426320667284524	No Hit
GTCAAACTGTGGTCCCTTCTCCTCGATGCAGTTCGCAAAAAAGCCATTCG	9	0.10426320667284524	No Hit
GTATAAGATCATATTTTGCATCCTGTTTGGGCTTTACTTTTTCTTTCAGA	9	0.10426320667284524	No Hit
GTACATGGGTACATTTGCTAATCCAGAGGCTGAGTGGAGCCGAGCTGGTC	9	0.10426320667284524	No Hit
GTCTGGTACTTGGGTTTGTGGCTGTTGCTATAGTGACCATCCCTTTCTCG	9	0.10426320667284524	No Hit
GCTCCAGGCAGTTCGAGTGACCGAGGAAGACACTCGGCACTGACCTCTTG	9	0.10426320667284524	No Hit
CTATAAATGCATTGAAAACTCAAACCACATGGCCAGAAAGTGCCATGCTC	9	0.10426320667284524	No Hit
GGTCAGGTTCGGGCTCCGGCTCTGGCTCCGGCTCTGGCTCGGGTTCCGGC	9	0.10426320667284524	No Hit
GAAGAGTACATGCCAATCAAAAATCCTAATCAGGATATCTACAGGGAAAT	9	0.10426320667284524	No Hit
TATCAACGCAGAGTACATGGGTACATTTGCTAATCCAGAGGCTGAGTGGA	9	0.10426320667284524	No Hit
GTCCAGGCGCGGGTGCTCAGGGTGAGCTGGCCGGGCTGATGGTGGGGCTG	9	0.10426320667284524	No Hit
GGATATCTACAGGGAAATGGGTTTTGGTCATTATGAAGAAGAAGAAAGCT	9	0.10426320667284524	No Hit
GTACTACGCTTGATCTGTTGATGGACTTTTAGGTTGTTTGTTAGTTTTTG	9	0.10426320667284524	No Hit
TTCCCATTCCATGTCACCTAGGAAGCCACTTCCGGAGCCGGAACCCGAGC	9	0.10426320667284524	No Hit
TCCTAACACTCCTCGTCCCCATTCTAATCGCCATAGCCTTCCTAACATTA	9	0.10426320667284524	No Hit
GGCTTATAGTTGAAATAGACAATATTGCTTTCATCTGTTGGCTGGTATTC	9	0.10426320667284524	No Hit
TAATGGGGGTGTGTGTTAATTTCTTAAATGTTGATTCAATACACATAAAT	9	0.10426320667284524	No Hit
GGATGCAGGTTCCCGTCGGCAGCAGGCTTGTCCTGGCTCTCGCCTTCGTC	9	0.10426320667284524	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.011584800741427247	0.0
23	0.0	0.0	0.0	0.011584800741427247	0.0
24	0.0	0.0	0.0	0.011584800741427247	0.0
25	0.0	0.0	0.0	0.011584800741427247	0.0
26	0.0	0.0	0.0	0.023169601482854494	0.0
27	0.0	0.0	0.0	0.03475440222428174	0.0
28	0.0	0.0	0.0	0.08109360518999073	0.0
29	0.0	0.0	0.0	0.08109360518999073	0.0
30	0.0	0.0	0.0	0.11584800741427248	0.0
31	0.0	0.0	0.0	0.18535681186283595	0.0
32	0.0	0.0	0.0	0.30120481927710846	0.0
33	0.0	0.0	0.0	0.45180722891566266	0.0
34	0.0	0.0	0.0	0.6835032437442076	0.0
35	0.0	0.0	0.0	0.892029657089898	0.0
36	0.0	0.0	0.0	1.1121408711770158	0.0
37	0.0	0.0	0.0	1.2974976830398517	0.0
38	0.0	0.0	0.0	1.59870250231696	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE