FastQCFastQC Report
Thu 2 Feb 2017
SRR4063870_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063870_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5215525
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT126270.24210410265505392No Hit
TATCAACGCAGAGTACTTTTTTTTT80950.15520968646492922No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG57660.11055454628249313No Hit
CTATTGGAGCTGGAATTACCGCGGC53220.10204150109528762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATT2600.012.4191832
AACCGCG4550.012.1102767
GTATCAA87600.011.9185291
CCGTCGT7100.011.90928659
CGTCGTA7250.011.79359210
GCGTAAC3850.011.59788211
TCGCGTA4150.011.2176559
CGGTCCA7750.011.15530110
GGTATCA34100.011.0323971
CGCGTAA4050.011.02514710
ACCGTCG7650.010.8045768
GGATACG803.7734467E-410.6876341
ACGCGCG2500.010.64074713
AGGCCCG11250.010.55599310
GTCCTAA6150.010.5041981
TCCAACG3750.010.38789418
CGCATCG7450.010.32958413
CGAGCCG10950.010.32512415
AAGACGG9200.010.3244345
TTGCGCG6300.010.25517918
Produced by FastQC (version 0.11.5)