##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063870_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5215525
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.226736522210132	32.0	32.0	32.0	32.0	32.0
2	30.75187119225773	32.0	32.0	32.0	32.0	32.0
3	30.784512009816844	32.0	32.0	32.0	32.0	32.0
4	30.819151475642432	32.0	32.0	32.0	32.0	32.0
5	30.683619578086578	32.0	32.0	32.0	32.0	32.0
6	34.36091706971015	36.0	36.0	36.0	32.0	36.0
7	34.29524295253115	36.0	36.0	36.0	32.0	36.0
8	34.24584945906692	36.0	36.0	36.0	32.0	36.0
9	34.420343877174396	36.0	36.0	36.0	32.0	36.0
10	34.05547035053998	36.0	36.0	36.0	32.0	36.0
11	34.395929844071304	36.0	36.0	36.0	32.0	36.0
12	34.17772707445559	36.0	36.0	36.0	32.0	36.0
13	34.28623407998236	36.0	36.0	36.0	32.0	36.0
14	34.16808988548612	36.0	36.0	36.0	32.0	36.0
15	34.08975625656094	36.0	36.0	36.0	32.0	36.0
16	34.09126751381692	36.0	36.0	36.0	32.0	36.0
17	34.00490803897978	36.0	36.0	36.0	32.0	36.0
18	34.005540573576006	36.0	36.0	36.0	32.0	36.0
19	34.025527823181754	36.0	36.0	36.0	32.0	36.0
20	34.003748424175896	36.0	36.0	36.0	32.0	36.0
21	33.99081185499063	36.0	36.0	36.0	32.0	36.0
22	33.967039751511116	36.0	36.0	36.0	32.0	36.0
23	33.901037575316	36.0	36.0	36.0	32.0	36.0
24	33.888461276669176	36.0	36.0	36.0	32.0	36.0
25	33.467158914970206	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	17.0
4	45.0
5	79.0
6	207.0
7	84.0
8	329.0
9	338.0
10	428.0
11	163.0
12	315.0
13	275.0
14	429.0
15	766.0
16	1332.0
17	1922.0
18	2651.0
19	3671.0
20	5640.0
21	8489.0
22	13440.0
23	20866.0
24	31129.0
25	44663.0
26	63300.0
27	83554.0
28	112531.0
29	151518.0
30	198516.0
31	267282.0
32	375148.0
33	522959.0
34	1088245.0
35	2215194.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.5138357009671	17.714316075415184	12.328248967618842	25.443599255998876
2	16.534473280381835	19.7219940968854	37.857111492857534	25.88642112987523
3	18.48988981549762	23.95967430514891	28.637981038586624	28.91245484076685
4	12.606774798530248	15.97313979505298	35.680408735913325	35.73967667050346
5	14.94159090643251	36.44590910075825	33.05871666627996	15.553783326529278
6	33.896275012181654	35.37203376000924	17.212902716091808	13.518788511717302
7	30.619414377440414	30.56117047799504	20.41365250811234	18.405762636452206
8	28.35584176004766	32.632554720977154	19.176792118115486	19.8348114008597
9	27.370443748911367	14.825622661112291	18.698229869164244	39.1057037208121
10	16.244221109769466	26.495413146766644	30.803954949883988	26.456410793579906
11	36.5943313481695	21.320348811779304	22.87722908882745	19.20809075122375
12	25.19466775539147	23.501759521002015	28.462492138657392	22.841080584949122
13	29.066918540757246	19.671178451488526	25.60088700814715	25.661015999607073
14	23.88401940850827	19.947597257912168	25.052739468846635	31.115643864732927
15	25.20721613250782	27.42596929801674	22.1820831427633	25.184731426712137
16	25.831398865969472	25.97140192427939	23.670781570862324	24.52641763888881
17	23.84351967160496	26.050432259793688	25.209549256857727	24.896498811743623
18	24.654804756660837	25.1749266186073	26.213616078838392	23.956652545893473
19	25.491192337175043	24.881117418555142	25.419660357410457	24.208029886859357
20	25.80354297098144	24.4310873742106	24.91909546571941	24.84627418908855
21	26.942131475154728	24.082850511987306	24.51368161709893	24.46133639575904
22	25.85361875558865	24.19462108507021	24.981233665805743	24.9705264935354
23	24.471696795294452	24.162075923988507	25.436520333465612	25.929706947251425
24	24.707830970435214	25.029405906663253	25.168673815675906	25.09408930722562
25	24.868469149565914	24.47015886934989	25.360303098043467	25.301068883040728
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	354.0
1	354.0
2	949.0
3	1544.0
4	1544.0
5	1544.0
6	3884.0
7	6224.0
8	6224.0
9	6224.0
10	6619.0
11	7014.0
12	7014.0
13	7014.0
14	9471.0
15	11928.0
16	11928.0
17	11928.0
18	21734.0
19	31540.0
20	31540.0
21	31540.0
22	52720.5
23	73901.0
24	73901.0
25	73901.0
26	113720.5
27	153540.0
28	153540.0
29	153540.0
30	197264.0
31	240988.0
32	240988.0
33	240988.0
34	291253.0
35	341518.0
36	341518.0
37	341518.0
38	387705.5
39	433893.0
40	433893.0
41	433893.0
42	491282.5
43	548672.0
44	548672.0
45	548672.0
46	610601.5
47	672531.0
48	672531.0
49	672531.0
50	687411.0
51	702291.0
52	702291.0
53	702291.0
54	652072.0
55	601853.0
56	601853.0
57	601853.0
58	550630.0
59	499407.0
60	499407.0
61	499407.0
62	438363.5
63	377320.0
64	377320.0
65	377320.0
66	309883.0
67	242446.0
68	242446.0
69	242446.0
70	184550.5
71	126655.0
72	126655.0
73	126655.0
74	97121.0
75	67587.0
76	67587.0
77	67587.0
78	54647.0
79	41707.0
80	41707.0
81	41707.0
82	28681.0
83	15655.0
84	15655.0
85	15655.0
86	11941.5
87	8228.0
88	8228.0
89	8228.0
90	6135.0
91	4042.0
92	4042.0
93	4042.0
94	2906.0
95	1770.0
96	1770.0
97	1770.0
98	2343.5
99	2917.0
100	2917.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.040111014710887204
2	0.005982139861279545
3	0.0017447907928732009
4	0.004333216694388389
5	0.009548415547811582
6	0.0137282440406287
7	0.023871038869528953
8	0.03543267456296346
9	0.049410174431145475
10	0.057884872567958164
11	0.05477876148613994
12	0.06348354192530953
13	0.06513246509220069
14	0.06871791430392914
15	0.05930371343249242
16	0.06703064408664516
17	0.06262073329147114
18	0.07772947114624128
19	0.07420154251010205
20	0.07930170021234678
21	0.07611889502974292
22	0.07794037992340176
23	0.08269541417211115
24	0.07644484495808188
25	0.07688583603759928
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	5215525.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.47820204717623
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.54171299386233	27.14318285331751
2	14.882891274939114	11.453337950465269
3	5.672466607219232	6.547989486553256
4	2.6480928844395923	4.075754121886253
5	1.4580006286738634	2.805062138751144
6	0.9578982300277822	2.2114920981384896
7	0.6506592071346207	1.7525337505186998
8	0.4624270441707352	1.4234688990144029
9	0.343154376291278	1.1883567081877682
>10	2.0666519181386147	14.926917350548036
>50	0.17730836646322382	4.704727539157565
>100	0.11125948322463813	8.405582128132966
>500	0.015465189536635008	4.239132055289702
>1k	0.01181159928037103	8.509865530808254
>5k	1.5014744847927094E-4	0.3694264607863134
>10k+	5.004914949309032E-5	0.2431709284443924
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	12627	0.24210410265505392	No Hit
TATCAACGCAGAGTACTTTTTTTTT	8095	0.15520968646492922	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	5766	0.11055454628249313	No Hit
CTATTGGAGCTGGAATTACCGCGGC	5322	0.10204150109528762	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.9173525196408797E-5	1.9173525196408797E-5
2	0.0	0.0	0.0	1.9173525196408797E-5	1.9173525196408797E-5
3	0.0	0.0	0.0	3.8347050392817595E-5	1.9173525196408797E-5
4	0.0	0.0	0.0	5.75205755892264E-5	1.9173525196408797E-5
5	0.0	0.0	0.0	7.669410078563519E-5	1.9173525196408797E-5
6	0.0	0.0	0.0	7.669410078563519E-5	1.9173525196408797E-5
7	0.0	0.0	0.0	9.586762598204399E-5	1.9173525196408797E-5
8	0.0	0.0	0.0	9.586762598204399E-5	1.9173525196408797E-5
9	0.0	0.0	0.0	1.150411511784528E-4	1.9173525196408797E-5
10	0.0	0.0	0.0	1.9173525196408797E-4	1.9173525196408797E-5
11	0.0	0.0	0.0	1.9173525196408797E-4	1.9173525196408797E-5
12	0.0	0.0	0.0	1.9173525196408797E-4	3.8347050392817595E-5
13	0.0	0.0	0.0	1.9173525196408797E-4	3.8347050392817595E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATT	260	0.0	12.419183	2
AACCGCG	455	0.0	12.110276	7
GTATCAA	8760	0.0	11.918529	1
CCGTCGT	710	0.0	11.9092865	9
CGTCGTA	725	0.0	11.793592	10
GCGTAAC	385	0.0	11.597882	11
TCGCGTA	415	0.0	11.217655	9
CGGTCCA	775	0.0	11.155301	10
GGTATCA	3410	0.0	11.032397	1
CGCGTAA	405	0.0	11.025147	10
ACCGTCG	765	0.0	10.804576	8
GGATACG	80	3.7734467E-4	10.687634	1
ACGCGCG	250	0.0	10.640747	13
AGGCCCG	1125	0.0	10.555993	10
GTCCTAA	615	0.0	10.504198	1
TCCAACG	375	0.0	10.387894	18
CGCATCG	745	0.0	10.329584	13
CGAGCCG	1095	0.0	10.325124	15
AAGACGG	920	0.0	10.324434	5
TTGCGCG	630	0.0	10.255179	18
>>END_MODULE