FastQCFastQC Report
Thu 2 Feb 2017
SRR4063870_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063870_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5215525
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA53520.10261670685117988No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA53150.10190728641891275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA93550.023.0014531
GGTATCA46900.018.1081771
GATATAC13250.017.7675741
TAGGACG8350.017.3880584
AACGCAG130900.016.3015886
GTATTAG15000.015.5480121
ACCGTCG14500.015.4749768
CGTCGTA14300.015.23062710
ATACCGT16150.015.1198776
TATCAAC143300.015.0597042
TCAACGC141150.015.0241454
CGACCCG14750.014.9142565
CAACGCA143550.014.7882835
AATAGCG9700.014.7412815
TACCGTC16000.014.7116577
ATAGCGT9900.014.6658266
CGCAATA14600.014.61784236
ATCAACG145150.014.5949583
TTAGGAC10000.014.5190293
CCGTCGT14850.014.5185219
Produced by FastQC (version 0.11.5)