##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063870_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5215525
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.309313635731783	32.0	32.0	32.0	32.0	32.0
2	31.385406071296753	32.0	32.0	32.0	32.0	32.0
3	31.471478134991205	32.0	32.0	32.0	32.0	32.0
4	31.56281908340963	32.0	32.0	32.0	32.0	32.0
5	31.501950235115352	32.0	32.0	32.0	32.0	32.0
6	35.09380743069969	36.0	36.0	36.0	36.0	36.0
7	35.10637816902421	36.0	36.0	36.0	36.0	36.0
8	35.05602734144693	36.0	36.0	36.0	36.0	36.0
9	35.17601334477354	36.0	36.0	36.0	36.0	36.0
10	35.02521318563328	36.0	36.0	36.0	36.0	36.0
11	35.176598137292025	36.0	36.0	36.0	36.0	36.0
12	35.07678498329506	36.0	36.0	36.0	36.0	36.0
13	35.122118099328446	36.0	36.0	36.0	36.0	36.0
14	35.06431375556632	36.0	36.0	36.0	36.0	36.0
15	35.03493032820282	36.0	36.0	36.0	36.0	36.0
16	35.050625775928594	36.0	36.0	36.0	36.0	36.0
17	35.00997962812948	36.0	36.0	36.0	36.0	36.0
18	35.01156853816251	36.0	36.0	36.0	36.0	36.0
19	35.00781397845854	36.0	36.0	36.0	36.0	36.0
20	35.00046476625076	36.0	36.0	36.0	36.0	36.0
21	34.99699685074849	36.0	36.0	36.0	36.0	36.0
22	34.981370811183915	36.0	36.0	36.0	36.0	36.0
23	34.931249874173744	36.0	36.0	36.0	32.0	36.0
24	34.89846237914687	36.0	36.0	36.0	32.0	36.0
25	34.875260112836195	36.0	36.0	36.0	32.0	36.0
26	34.816327215381	36.0	36.0	36.0	32.0	36.0
27	34.813418399873456	36.0	36.0	36.0	32.0	36.0
28	34.788611309503835	36.0	36.0	36.0	32.0	36.0
29	34.7605855594595	36.0	36.0	36.0	32.0	36.0
30	34.74092751928138	36.0	36.0	36.0	32.0	36.0
31	34.73950676106432	36.0	36.0	36.0	32.0	36.0
32	34.69795178817089	36.0	36.0	36.0	32.0	36.0
33	34.67670828919428	36.0	36.0	36.0	32.0	36.0
34	34.66925918292022	36.0	36.0	36.0	32.0	36.0
35	34.63671001481155	36.0	36.0	36.0	32.0	36.0
36	34.61024920789374	36.0	36.0	36.0	32.0	36.0
37	34.60119489409024	36.0	36.0	36.0	32.0	36.0
38	34.55773426452754	36.0	36.0	36.0	32.0	36.0
39	34.56222949750984	36.0	36.0	36.0	32.0	36.0
40	34.54128453032053	36.0	36.0	36.0	32.0	36.0
41	34.5300106892403	36.0	36.0	36.0	32.0	36.0
42	34.45858547317864	36.0	36.0	36.0	32.0	36.0
43	34.45232301637898	36.0	36.0	36.0	32.0	36.0
44	34.391041170351976	36.0	36.0	36.0	32.0	36.0
45	34.36163799425753	36.0	36.0	36.0	32.0	36.0
46	34.34296758236228	36.0	36.0	36.0	32.0	36.0
47	34.30946031319954	36.0	36.0	36.0	32.0	36.0
48	34.265297932614644	36.0	36.0	36.0	32.0	36.0
49	34.24889919998466	36.0	36.0	36.0	32.0	36.0
50	33.637422311272594	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	4.0
18	8.0
19	15.0
20	67.0
21	191.0
22	559.0
23	1512.0
24	3998.0
25	9063.0
26	18623.0
27	34077.0
28	57127.0
29	88464.0
30	128330.0
31	184753.0
32	269841.0
33	439959.0
34	919472.0
35	3059461.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.53336703081939	18.670120275480784	12.92167558831899	25.874837105380834
2	15.77236174535802	20.426965529499263	37.389778128782595	26.41089459636012
3	17.68142696166677	24.543797422337757	28.979574541352786	28.795201074642684
4	12.25109648597217	16.615240076502367	35.76861389792974	35.36504953959573
5	14.47025946572972	36.88317091759698	32.9012132048068	15.745356411866496
6	33.930954773541764	35.69454103137393	16.971363677063923	13.40314051802039
7	30.49076765647472	30.695291728173764	20.462952612806042	18.350988002545478
8	28.27283093181643	32.891965005235335	19.020777015075957	19.814427047872286
9	27.36174413862486	14.668992573421658	18.607573602850145	39.36168968510334
10	16.155860570509113	26.67906820500962	30.847791249207646	26.317279975273618
11	36.87062136265965	21.20146769613237	22.76967152466647	19.158239416541512
12	25.139247913630054	23.50307294500757	28.426764129660754	22.930915011701618
13	29.118747793835258	19.64772690014896	25.650738309028533	25.58278699698725
14	23.912599985351395	19.87065896093846	25.024225800179998	31.19251525353014
15	25.223588487122345	27.504964744894465	22.124134249539765	25.147312518443428
16	25.899732604428266	26.006625642748322	23.599528635254877	24.494113117568535
17	23.9017402661746	26.174244428747716	25.081679232997487	24.842336072080197
18	24.702009064883576	25.129445609452265	26.183781416624374	23.984763909039785
19	25.58979201518543	24.844459570225432	25.341782466769885	24.223965947819252
20	25.91018790376128	24.37763745643026	24.902075337518685	24.810099302289775
21	27.02916772520504	24.037503415284174	24.46488129191213	24.46844756759866
22	25.86811690431986	24.112450683183035	25.0590881472254	24.9603442652717
23	24.494423041267368	24.05269930247991	25.486949707572286	25.965927948680438
24	24.814935963855376	24.975572774342286	25.142882013815765	25.066609247986577
25	24.956695804564532	24.37181593847487	25.427318552832652	25.244169704127945
26	24.583254477796103	25.241460928016064	25.847800088701696	24.32748450548614
27	25.067116120811896	24.85193803131798	25.065582147619992	25.01536370025013
28	24.48612108446614	24.79722584671787	25.69027387765002	25.026379191165965
29	24.55274189518749	24.540892347627377	25.529792696191983	25.376573060993152
30	24.464424453782737	24.896113836839284	25.7249789274837	24.91448278189428
31	25.3033822225793	24.612410718754177	24.76933900479011	25.31486805387641
32	24.828468216959134	24.874735396981222	24.705811295864525	25.59098509019512
33	24.48905085696032	24.62049119378505	25.23117584550363	25.659282103751007
34	25.149389451805664	24.648775404355757	25.548918154808987	24.652916989029595
35	25.615563494461767	24.585322959920113	25.297236023272685	24.50187752234543
36	24.60961438898583	25.100439109508926	24.955686372265156	25.334260129240093
37	25.493800470345512	24.790678990626645	24.963861243280608	24.751659295747235
38	24.808192038828697	24.68957521909405	25.318746749830108	25.183485992247146
39	25.307499796751397	24.57536381499929	24.797767640061295	25.31936874818802
40	25.584678567477354	24.9102511472303	25.139610924366686	24.365459360925662
41	24.58252002745746	25.008455824548353	25.800043717188142	24.608980430806046
42	25.825723219647557	25.236893309619074	24.91461870435178	24.02276476638159
43	24.887157106873307	24.378858377543438	25.409970439677444	25.324014075905815
44	24.608897077391127	24.933855091504213	24.946298905001935	25.510948926102728
45	24.684360284079577	25.10135290598684	25.085151013145584	25.129135796788
46	24.72928645439074	24.753234412352775	25.049985849805463	25.46749328345102
47	24.97055844804769	24.925480565507886	25.3836030017153	24.720357984729123
48	25.59941082326558	25.24026381036363	24.43037588777725	24.729949478593547
49	24.82118208778025	25.340192629624653	24.805766502585275	25.03285878000982
50	24.844400140275233	25.420111472408667	24.586858189525337	25.148630197790762
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	45.0
1	73.0
2	101.0
3	288.5
4	476.0
5	542.0
6	608.0
7	602.0
8	596.0
9	807.0
10	1018.0
11	1556.5
12	2095.0
13	4115.5
14	6136.0
15	8333.5
16	10531.0
17	11265.0
18	11999.0
19	12022.0
20	12045.0
21	13684.5
22	15324.0
23	17557.0
24	19790.0
25	24326.5
26	28863.0
27	38633.0
28	48403.0
29	58093.5
30	67784.0
31	76561.5
32	85339.0
33	99343.5
34	113348.0
35	132628.0
36	151908.0
37	177521.5
38	203135.0
39	221123.5
40	239112.0
41	258193.5
42	277275.0
43	281770.0
44	286265.0
45	313067.0
46	339869.0
47	362483.0
48	385097.0
49	394281.5
50	403466.0
51	391240.0
52	379014.0
53	374812.5
54	370611.0
55	370158.5
56	369706.0
57	360795.0
58	351884.0
59	319728.5
60	287573.0
61	253863.0
62	220153.0
63	191549.5
64	162946.0
65	138896.5
66	114847.0
67	98452.5
68	82058.0
69	74338.5
70	66619.0
71	51099.0
72	35579.0
73	30533.5
74	25488.0
75	19226.5
76	12965.0
77	11279.0
78	9593.0
79	8139.5
80	6686.0
81	5317.0
82	3948.0
83	3304.0
84	2660.0
85	1903.5
86	1147.0
87	802.5
88	458.0
89	363.0
90	268.0
91	232.0
92	196.0
93	177.0
94	158.0
95	151.0
96	144.0
97	131.0
98	118.0
99	98.0
100	78.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009529242022615174
2	4.2181755432099357E-4
3	4.601646047138112E-4
4	0.0
5	0.0
6	3.0677640314254076E-4
7	3.8347050392817595E-5
8	3.8347050392817595E-5
9	0.0013037997133557983
10	8.244615834455784E-4
11	7.669410078563519E-5
12	1.342146763748616E-4
13	4.985116551066288E-4
14	2.1090877716049678E-4
15	0.004831728349495017
16	5.943792810886728E-4
17	1.9173525196408797E-5
18	2.684293527497232E-4
19	0.0
20	6.902469070707168E-4
21	0.0
22	2.300823023569056E-4
23	9.203292094276224E-4
24	6.327263314814904E-4
25	0.0018214848936588361
26	0.0055986693573513695
27	0.005943792810886727
28	0.004601646047138112
29	0.0026075994267115966
30	0.0038538785644781685
31	0.007746104179349154
32	0.003163631657407452
33	0.004429084320370432
34	0.002492558275533144
35	0.0027034670526936404
36	0.008148748208473739
37	0.004026440291245848
38	0.007918665906116833
39	0.004544125471548885
40	0.0026459464771044143
41	0.003547102161335628
42	0.0018598319440516536
43	0.0016872702172839743
44	0.0015722290661055215
45	0.001629749641694748
46	9.39502734624031E-4
47	0.0020515671960157416
48	0.0021474348219977855
49	4.601646047138112E-4
50	0.001227105612570163
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	5215525.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.87331414133622
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.04952563543576	30.16952420666859
2	14.400724124390814	12.060120902466712
3	5.34755922661782	6.7176008215670695
4	2.480013980915063	4.15385617991049
5	1.4293966811540428	2.9926788131273323
6	0.8727110453731028	2.192598225451312
7	0.5721834038256798	1.6771450790396214
8	0.41587838033778923	1.3931364851579484
9	0.318376512794554	1.199833176192256
>10	1.8489725454555035	14.473537519091312
>50	0.14948135154092068	4.333438467452614
>100	0.09271024382335288	7.538525307537883
>500	0.013351214964617199	3.895392096796662
>1k	0.008977537411203907	6.898098853799805
>5k	1.381159601723046E-4	0.30451386574012246
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	5352	0.10261670685117988	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	5315	0.10190728641891275	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.342146763748616E-4
2	0.0	0.0	0.0	0.0	1.342146763748616E-4
3	0.0	0.0	0.0	0.0	1.342146763748616E-4
4	0.0	0.0	0.0	1.9173525196408797E-5	1.342146763748616E-4
5	0.0	0.0	0.0	3.8347050392817595E-5	1.342146763748616E-4
6	0.0	0.0	0.0	3.8347050392817595E-5	1.342146763748616E-4
7	0.0	0.0	0.0	3.8347050392817595E-5	1.342146763748616E-4
8	0.0	0.0	0.0	3.8347050392817595E-5	1.342146763748616E-4
9	0.0	0.0	0.0	5.75205755892264E-5	1.342146763748616E-4
10	0.0	0.0	0.0	1.342146763748616E-4	1.342146763748616E-4
11	0.0	0.0	0.0	1.5338820157127038E-4	1.342146763748616E-4
12	0.0	0.0	0.0	1.5338820157127038E-4	2.492558275533144E-4
13	0.0	0.0	0.0	1.5338820157127038E-4	2.492558275533144E-4
14	0.0	0.0	0.0	1.725617267676792E-4	2.492558275533144E-4
15	0.0	0.0	0.0	1.725617267676792E-4	2.684293527497232E-4
16	0.0	0.0	0.0	1.725617267676792E-4	2.684293527497232E-4
17	0.0	0.0	0.0	1.725617267676792E-4	2.684293527497232E-4
18	0.0	0.0	0.0	1.9173525196408797E-4	2.684293527497232E-4
19	0.0	0.0	0.0	2.492558275533144E-4	2.87602877946132E-4
20	0.0	0.0	0.0	2.492558275533144E-4	3.2594992833894957E-4
21	0.0	0.0	0.0	3.451234535353584E-4	3.451234535353584E-4
22	0.0	0.0	0.0	4.7933812991021995E-4	3.451234535353584E-4
23	0.0	0.0	0.0	8.436351086419871E-4	3.451234535353584E-4
24	0.0	0.0	0.0	0.0015338820157127038	3.451234535353584E-4
25	0.0	0.0	0.0	0.001802311368462427	3.451234535353584E-4
26	0.0	0.0	0.0	0.002281649498372647	3.8347050392817595E-4
27	0.0	0.0	0.0	0.003144458132211043	3.8347050392817595E-4
28	0.0	0.0	0.0	0.004275696118799162	4.0264402912458476E-4
29	0.0	0.0	0.0	0.006902469070707167	4.0264402912458476E-4
30	0.0	0.0	0.0	0.013766591091021518	4.0264402912458476E-4
31	0.0	0.0	0.0	0.032959289812626726	4.0264402912458476E-4
32	0.0	0.0	0.0	0.054567852708979445	4.0264402912458476E-4
33	0.0	0.0	0.0	0.0771159183399562	4.0264402912458476E-4
34	0.0	0.0	0.0	0.10282761562834039	4.0264402912458476E-4
35	0.0	0.0	0.0	0.1325274061575776	4.0264402912458476E-4
36	0.0	0.0	0.0	0.17622387008019327	4.0264402912458476E-4
37	0.0	0.0	0.0	0.23765584480948707	4.0264402912458476E-4
38	0.0	0.0	0.0	0.3243010051720584	4.0264402912458476E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	9355	0.0	23.001453	1
GGTATCA	4690	0.0	18.108177	1
GATATAC	1325	0.0	17.767574	1
TAGGACG	835	0.0	17.388058	4
AACGCAG	13090	0.0	16.301588	6
GTATTAG	1500	0.0	15.548012	1
ACCGTCG	1450	0.0	15.474976	8
CGTCGTA	1430	0.0	15.230627	10
ATACCGT	1615	0.0	15.119877	6
TATCAAC	14330	0.0	15.059704	2
TCAACGC	14115	0.0	15.024145	4
CGACCCG	1475	0.0	14.914256	5
CAACGCA	14355	0.0	14.788283	5
AATAGCG	970	0.0	14.741281	5
TACCGTC	1600	0.0	14.711657	7
ATAGCGT	990	0.0	14.665826	6
CGCAATA	1460	0.0	14.617842	36
ATCAACG	14515	0.0	14.594958	3
TTAGGAC	1000	0.0	14.519029	3
CCGTCGT	1485	0.0	14.518521	9
>>END_MODULE