FastQCFastQC Report
Thu 2 Feb 2017
SRR4063869_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063869_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2772456
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66160.23863318299731356No Hit
TATCAACGCAGAGTACTTTTTTTTT48230.1739612819824733No Hit
GTCCTAAAGTGTGTATTTCTCATTT40830.14727014603658273No Hit
GTCCTACAGTGGACATTTCTAAATT36900.13309498870315706No Hit
CTTTAGGACGTGAAATATGGCGAGG35640.12855028177182973No Hit
CTGTAGGACGTGGAATATGGCAAGA34010.1226710180432079No Hit
CTATTGGAGCTGGAATTACCGCGGC33880.12220211970902335No Hit
ACGCAGAGTACTTTTTTTTTTTTTT33470.12072328650121049No Hit
GAATAGGACCGCGGTTCTATTTTGT31100.11217490917799958No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG30760.1109485596885938No Hit
GTATCTGATCGTCTTCGAACCTCCG30630.11047966135440924No Hit
GTACTTTTTTTTTTTTTTTTTTTTT29240.10546605608889735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTA4650.013.48581810
ACCGAGT1351.8189894E-1213.372068
GGACCGA1450.013.1015566
GGACCGT905.4195516E-712.6648396
CCGTCGT4850.012.5378929
CGAACGA2300.012.39376516
GTATCAA50550.012.3433931
ACCGTCG5100.012.1093728
ATCGGCG550.003066170312.09265617
AACCGCG2200.012.0909097
CGCATCG5150.011.992022513
CGCCAGT5450.011.85492218
CGAACGT1552.5465852E-1111.6417994
TACCGTC5550.011.639647
TTACACA3300.011.5118274
CCGACCA4050.011.4954879
ACTCTAA5400.011.43683710
CGACCAT4000.011.40164810
GGCGAGG10750.011.40123519
GCGTTAT1604.5474735E-1111.2777891
Produced by FastQC (version 0.11.5)