##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063869_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2772456
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.157968602567543	32.0	32.0	32.0	32.0	32.0
2	30.740698499813885	32.0	32.0	32.0	32.0	32.0
3	30.752569562871333	32.0	32.0	32.0	32.0	32.0
4	30.73385763380916	32.0	32.0	32.0	32.0	32.0
5	30.689949993796116	32.0	32.0	32.0	32.0	32.0
6	34.12767993432538	36.0	36.0	36.0	32.0	36.0
7	34.088855152254894	36.0	36.0	36.0	32.0	36.0
8	34.02442130731741	36.0	36.0	36.0	32.0	36.0
9	34.24204171319581	36.0	36.0	36.0	32.0	36.0
10	33.84848884887623	36.0	36.0	36.0	32.0	36.0
11	34.21873710529581	36.0	36.0	36.0	32.0	36.0
12	33.988289083758225	36.0	36.0	36.0	32.0	36.0
13	34.11645775442424	36.0	36.0	36.0	32.0	36.0
14	33.96541946923594	36.0	36.0	36.0	32.0	36.0
15	33.87459963296081	36.0	36.0	36.0	32.0	36.0
16	33.92256973600303	36.0	36.0	36.0	32.0	36.0
17	33.81164389984909	36.0	36.0	36.0	32.0	36.0
18	33.827657499343545	36.0	36.0	36.0	32.0	36.0
19	33.82536170096117	36.0	36.0	36.0	32.0	36.0
20	33.760749674656694	36.0	36.0	36.0	32.0	36.0
21	33.74126225988798	36.0	36.0	36.0	32.0	36.0
22	33.70735008959565	36.0	36.0	36.0	32.0	36.0
23	33.66065827555063	36.0	36.0	36.0	27.0	36.0
24	33.637591002345935	36.0	36.0	36.0	27.0	36.0
25	32.91669011158338	36.0	32.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	6.0
4	19.0
5	40.0
6	103.0
7	43.0
8	154.0
9	168.0
10	235.0
11	69.0
12	164.0
13	155.0
14	408.0
15	999.0
16	1585.0
17	2122.0
18	2566.0
19	3222.0
20	4134.0
21	5701.0
22	7608.0
23	10558.0
24	14930.0
25	20589.0
26	28980.0
27	39740.0
28	56202.0
29	79407.0
30	112595.0
31	166091.0
32	258188.0
33	387288.0
34	746163.0
35	822223.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.7613003579672	18.428725703892106	12.254955199075729	25.555018739064973
2	15.526718074910598	19.965169940822083	39.5647974005868	24.943314583680515
3	18.833160860232585	24.790714803313936	28.64075446190737	27.735369874546105
4	11.922787666081724	16.09353780450195	36.5678637836785	35.41581074573783
5	13.71007682981685	37.63730367346733	33.842490037807096	14.810129458908728
6	33.117937918070375	36.35615601251176	17.52859102683839	12.997315042579464
7	29.64749683428996	30.947476270703383	21.070713484301326	18.33431341070533
8	27.3237756555599	34.33609090161225	19.323628444133988	19.016504998693858
9	27.128555953057127	14.611298073973783	19.227893495881567	39.032252477087525
10	15.598447907013	27.262169754396297	31.72851508931175	25.41086724927895
11	36.243811374260154	21.141994744878314	23.702576424272586	18.91161745658895
12	25.35541628395046	23.57089107159936	29.499614727340646	21.574077917109534
13	29.260588491755286	20.28462391328827	25.858975062293737	24.595812532662706
14	23.109955837492365	20.893240494377547	25.700911979603536	30.295891688526556
15	24.253551838417202	29.296951267264763	22.5079748773769	23.941522016941132
16	24.60965992655352	26.247714948253613	25.539452670329965	23.603172454862897
17	22.70675676846187	27.00170204042743	26.287141005171083	24.004400185939616
18	23.53804293682203	25.537035138658876	28.220261652124822	22.70466027239427
19	25.193595590055967	24.91595276751276	25.975630092554823	23.914821549876446
20	25.489196703097576	25.03643933383339	25.804282334629676	23.67008162843936
21	26.152695572577716	24.519311634769966	24.9331710525081	24.394821740144216
22	25.315208783180392	25.52694823031881	25.46063978088252	23.69720320561828
23	23.26937411898905	25.534039285192158	26.473519585742107	24.72306701007669
24	24.446112863753513	25.470686755076848	26.289479573494273	23.79372080767537
25	23.900543176935336	25.56465422644212	26.38884076050546	24.14596183611708
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	207.0
1	207.0
2	580.5
3	954.0
4	954.0
5	954.0
6	2588.5
7	4223.0
8	4223.0
9	4223.0
10	4456.5
11	4690.0
12	4690.0
13	4690.0
14	5822.0
15	6954.0
16	6954.0
17	6954.0
18	13047.0
19	19140.0
20	19140.0
21	19140.0
22	34136.0
23	49132.0
24	49132.0
25	49132.0
26	75964.0
27	102796.0
28	102796.0
29	102796.0
30	133662.0
31	164528.0
32	164528.0
33	164528.0
34	190114.0
35	215700.0
36	215700.0
37	215700.0
38	235774.5
39	255849.0
40	255849.0
41	255849.0
42	280694.0
43	305539.0
44	305539.0
45	305539.0
46	331236.5
47	356934.0
48	356934.0
49	356934.0
50	355192.5
51	353451.0
52	353451.0
53	353451.0
54	320499.5
55	287548.0
56	287548.0
57	287548.0
58	259801.0
59	232054.0
60	232054.0
61	232054.0
62	203522.5
63	174991.0
64	174991.0
65	174991.0
66	143315.5
67	111640.0
68	111640.0
69	111640.0
70	84786.0
71	57932.0
72	57932.0
73	57932.0
74	44706.5
75	31481.0
76	31481.0
77	31481.0
78	26511.0
79	21541.0
80	21541.0
81	21541.0
82	14604.5
83	7668.0
84	7668.0
85	7668.0
86	5938.0
87	4208.0
88	4208.0
89	4208.0
90	2918.5
91	1629.0
92	1629.0
93	1629.0
94	1041.5
95	454.0
96	454.0
97	454.0
98	833.5
99	1213.0
100	1213.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03509523685858315
2	0.0049775361628822965
3	0.00155097141307202
4	0.0036429793655877677
5	0.00811554809165592
6	0.01244384040720574
7	0.020415112088343332
8	0.03361640365077029
9	0.04537493110801398
10	0.053670824712817804
11	0.052119853299745784
12	0.06099285254662293
13	0.061750303701844145
14	0.06420300268065571
15	0.056520283820554774
16	0.0624356166518062
17	0.06001898677562421
18	0.07430235141693863
19	0.07177751423286789
20	0.07567297731686273
21	0.07033475012768461
22	0.07466304244323445
23	0.07938809488770968
24	0.07343669295382865
25	0.07531228629056692
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2772456.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.83932308330086
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.98032262848767	26.68450713420048
2	16.125614852729317	12.848671600695166
3	6.898227666167892	8.244621620838814
4	3.4476089084150017	5.494016206688459
5	1.900871820498683	3.786472329839466
6	1.1672670501805595	2.7901877481980923
7	0.7808445598604232	2.1775823088682085
8	0.5012760114618526	1.597639757962974
9	0.3748997123981011	1.3442175689458074
>10	1.5804102249582312	10.759633399842182
>50	0.11021361436944728	3.0585876044942237
>100	0.10426858310672737	8.932299580923065
>500	0.01802797140140855	4.9795895482447365
>1k	0.010055803143496038	7.063191785015389
>5k	9.059282111257691E-5	0.23878180524298073
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6616	0.23863318299731356	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4823	0.1739612819824733	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4083	0.14727014603658273	No Hit
GTCCTACAGTGGACATTTCTAAATT	3690	0.13309498870315706	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3564	0.12855028177182973	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3401	0.1226710180432079	No Hit
CTATTGGAGCTGGAATTACCGCGGC	3388	0.12220211970902335	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3347	0.12072328650121049	No Hit
GAATAGGACCGCGGTTCTATTTTGT	3110	0.11217490917799958	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	3076	0.1109485596885938	No Hit
GTATCTGATCGTCTTCGAACCTCCG	3063	0.11047966135440924	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2924	0.10546605608889735	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.213820525916372E-5
2	0.0	0.0	0.0	0.0	7.213820525916372E-5
3	0.0	0.0	0.0	0.0	7.213820525916372E-5
4	0.0	0.0	0.0	3.606910262958186E-5	7.213820525916372E-5
5	0.0	0.0	0.0	3.606910262958186E-5	7.213820525916372E-5
6	0.0	0.0	0.0	3.606910262958186E-5	7.213820525916372E-5
7	0.0	0.0	0.0	3.606910262958186E-5	7.213820525916372E-5
8	0.0	0.0	0.0	7.213820525916372E-5	7.213820525916372E-5
9	0.0	0.0	0.0	1.8034551314790928E-4	7.213820525916372E-5
10	0.0	0.0	0.0	1.8034551314790928E-4	7.213820525916372E-5
11	0.0	3.606910262958186E-5	0.0	2.1641461577749115E-4	7.213820525916372E-5
12	0.0	3.606910262958186E-5	0.0	2.52483718407073E-4	2.1641461577749115E-4
13	0.0	3.606910262958186E-5	0.0	2.52483718407073E-4	2.1641461577749115E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCGTA	465	0.0	13.485818	10
ACCGAGT	135	1.8189894E-12	13.37206	8
GGACCGA	145	0.0	13.101556	6
GGACCGT	90	5.4195516E-7	12.664839	6
CCGTCGT	485	0.0	12.537892	9
CGAACGA	230	0.0	12.393765	16
GTATCAA	5055	0.0	12.343393	1
ACCGTCG	510	0.0	12.109372	8
ATCGGCG	55	0.0030661703	12.092656	17
AACCGCG	220	0.0	12.090909	7
CGCATCG	515	0.0	11.9920225	13
CGCCAGT	545	0.0	11.854922	18
CGAACGT	155	2.5465852E-11	11.641799	4
TACCGTC	555	0.0	11.63964	7
TTACACA	330	0.0	11.511827	4
CCGACCA	405	0.0	11.495487	9
ACTCTAA	540	0.0	11.436837	10
CGACCAT	400	0.0	11.401648	10
GGCGAGG	1075	0.0	11.401235	19
GCGTTAT	160	4.5474735E-11	11.277789	1
>>END_MODULE