FastQCFastQC Report
Thu 2 Feb 2017
SRR4063869_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063869_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2772456
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA31810.1147358154646999No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT30560.11022717763600215No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA29230.10542998698626777No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA28580.10308549531534494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61050.021.7287861
TTAGGAC18000.019.0654963
TGTAGGA30550.018.865932
GATATAC11350.018.8009451
TAGGACG28800.018.7137894
GTAGGAC30800.018.0703163
AGGACGT30750.018.0278365
GACGTGA16250.018.0047237
ACCGTCG8200.017.974188
GTCCTAC24300.017.8346161
ATACACT12150.017.7434464
GGACGTG30600.017.3973086
CTGTAGG29850.017.392881
GTCCTAA16000.017.1867541
AACGCAG77500.017.1444426
CGTCGTA8600.017.1387910
TTTAGGA21100.016.993912
CCGTCGT8550.016.981729
GCGAGAA17050.016.90310120
ATACCGT8850.016.9026096
Produced by FastQC (version 0.11.5)