##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063869_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2772456
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.086630049313676	32.0	32.0	32.0	32.0	32.0
2	30.91441884019079	32.0	32.0	32.0	32.0	32.0
3	31.212690480930988	32.0	32.0	32.0	32.0	32.0
4	31.386578181944095	32.0	32.0	32.0	32.0	32.0
5	31.242914224788418	32.0	32.0	32.0	32.0	32.0
6	34.835030384612054	36.0	36.0	36.0	32.0	36.0
7	34.87096062119652	36.0	36.0	36.0	32.0	36.0
8	34.783281682378366	36.0	36.0	36.0	32.0	36.0
9	34.98935384366785	36.0	36.0	36.0	36.0	36.0
10	34.73611844516198	36.0	36.0	36.0	32.0	36.0
11	34.99753143061603	36.0	36.0	36.0	36.0	36.0
12	34.85900154952865	36.0	36.0	36.0	32.0	36.0
13	34.929940457125376	36.0	36.0	36.0	32.0	36.0
14	34.85462239977839	36.0	36.0	36.0	32.0	36.0
15	34.794755263924834	36.0	36.0	36.0	32.0	36.0
16	34.83018774689301	36.0	36.0	36.0	32.0	36.0
17	34.77638238442738	36.0	36.0	36.0	32.0	36.0
18	34.78463896271032	36.0	36.0	36.0	32.0	36.0
19	34.78511399279195	36.0	36.0	36.0	32.0	36.0
20	34.77573350127108	36.0	36.0	36.0	32.0	36.0
21	34.770848302010926	36.0	36.0	36.0	32.0	36.0
22	34.754963829903886	36.0	36.0	36.0	32.0	36.0
23	34.69451309596978	36.0	36.0	36.0	32.0	36.0
24	34.676321644058554	36.0	36.0	36.0	32.0	36.0
25	34.62882476764284	36.0	36.0	36.0	32.0	36.0
26	34.563087385336324	36.0	36.0	36.0	32.0	36.0
27	34.557212449900014	36.0	36.0	36.0	32.0	36.0
28	34.51930706925556	36.0	36.0	36.0	32.0	36.0
29	34.489346629847326	36.0	36.0	36.0	32.0	36.0
30	34.45777426224257	36.0	36.0	36.0	32.0	36.0
31	34.46350275712221	36.0	36.0	36.0	32.0	36.0
32	34.412538197179686	36.0	36.0	36.0	32.0	36.0
33	34.38121218154589	36.0	36.0	36.0	32.0	36.0
34	34.38174888979302	36.0	36.0	36.0	32.0	36.0
35	34.338603029227514	36.0	36.0	36.0	32.0	36.0
36	34.30018907423599	36.0	36.0	36.0	32.0	36.0
37	34.28043907640013	36.0	36.0	36.0	32.0	36.0
38	34.22178133755774	36.0	36.0	36.0	32.0	36.0
39	34.21363837694809	36.0	36.0	36.0	32.0	36.0
40	34.20514013567754	36.0	36.0	36.0	32.0	36.0
41	34.17862970593582	36.0	36.0	36.0	32.0	36.0
42	34.094872560646586	36.0	36.0	36.0	32.0	36.0
43	34.1004430728567	36.0	36.0	36.0	32.0	36.0
44	34.00772996938455	36.0	36.0	36.0	32.0	36.0
45	33.97046589738485	36.0	36.0	36.0	32.0	36.0
46	33.92006076922411	36.0	36.0	36.0	32.0	36.0
47	33.90656082549191	36.0	36.0	36.0	32.0	36.0
48	33.85010041638172	36.0	36.0	36.0	32.0	36.0
49	33.822476172750804	36.0	36.0	36.0	32.0	36.0
50	33.11590878268221	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	5.0
18	5.0
19	8.0
20	25.0
21	85.0
22	267.0
23	803.0
24	2061.0
25	4722.0
26	10253.0
27	19580.0
28	33553.0
29	54717.0
30	83624.0
31	127806.0
32	194622.0
33	328527.0
34	641644.0
35	1270149.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.82894809459173	19.370030512647833	13.118418579854293	25.68260281290614
2	15.042168539982384	20.933248113759475	38.918918528981266	25.105664817276875
3	17.85719824919881	25.60429512578248	29.18957815213647	27.34892847288224
4	11.837735206618248	17.031000672328073	37.07427638166305	34.056987739390635
5	13.825900212663427	37.69513384522604	33.34844628733513	15.130519654775403
6	33.203953322202395	36.96744363204714	17.052335963855835	12.776267081894627
7	29.72527177235722	30.925762746221842	20.99991740154643	18.349048079874507
8	27.318810469850558	34.59957525024743	19.005603695784533	19.076010584117476
9	27.192722060370798	14.374991208083296	18.765106316365447	39.66718041518046
10	15.6027135781344	27.373479130927837	31.560603196908993	25.463204094028775
11	36.72701630865063	21.07413826374098	23.59086080747929	18.60798462012909
12	25.476482034875225	23.445285856758723	29.414003112764682	21.66422899560137
13	29.46258627312715	20.233476227661864	25.639534778868473	24.664402720342512
14	23.093353993782387	20.768800990605047	25.603338855530872	30.534506160081687
15	24.21362266512526	29.307684954903124	22.379520734102474	24.09917164586914
16	24.673734813907473	26.36618023559048	25.25394616579343	23.70613878470862
17	22.945143223192723	27.00728163043886	25.96614698303598	24.08142816333244
18	23.819490933644204	25.457465748681308	27.982779117949192	22.740264199725296
19	25.414154603050363	24.8591591207071	25.94318032213327	23.783505954109263
20	25.82093869795371	24.874695033537293	25.651953733106915	23.652412535402078
21	26.44117706466757	24.44897231912788	24.890313858903443	24.219536757301107
22	25.50247939369194	25.317120007848647	25.50247939369194	23.677921204767475
23	23.464915523779258	25.421930236827745	26.42877006763364	24.68438417175936
24	24.767110065584664	25.393055304573902	26.09085042924175	23.748984200599686
25	24.258461050133278	25.3311378908603	26.34538181582089	24.06501924318553
26	23.373417748914626	26.514365606229596	26.83770536980633	23.274511275049452
27	24.215081162056105	25.726717919065557	25.914721135175828	24.143479783702514
28	23.325210254989635	26.058128344637833	26.1524527223855	24.464208677987035
29	23.768082799437018	25.209693022544478	26.165440165979774	24.856784012038727
30	23.339409989618947	25.645461372593292	26.717545136667052	24.297583501120705
31	24.806567018170618	25.0599326618759	25.647391783940858	24.486108536012622
32	23.96481697903591	25.949263443420044	25.38703487281594	24.6988847047281
33	23.20372019606634	25.688816651138197	26.603824152057097	24.50363900073837
34	24.31198931458456	25.653365325134352	26.55576784831212	23.47887751196897
35	24.6023461379476	25.724592462954295	26.23729163255045	23.435769766547658
36	23.357127372114405	26.010158487849093	26.29595388316332	24.336760256873173
37	24.502326366557746	25.833075128708543	25.39644178997666	24.26815671475705
38	23.996219670339382	26.071577367801186	25.77351568854845	24.15868727331098
39	24.958690146702732	25.086560419515493	25.420502023380244	24.534247410401537
40	25.504039821093638	25.456932621555335	25.930493435290725	23.10853412206031
41	24.206642492343175	25.42069707321877	26.860046826479795	23.512613607958265
42	25.235147992553753	26.374737006127898	25.80501463892515	22.585100362393202
43	23.86625100048081	25.697324753094502	26.361005577443798	24.075418668980895
44	23.515541898760247	26.232529183103935	25.54352550435379	24.708403413782023
45	23.750926088239346	26.443886086277157	25.60407644745698	24.201111378026514
46	23.80476435061881	25.556949442076682	25.650369496152102	24.98791671115241
47	24.78841345439383	25.481931100553023	25.864020476210403	23.865634968842745
48	24.690250548080687	26.029919181994533	25.028116413132008	24.251713856792772
49	24.409680003549227	26.040916349045983	25.181672297693332	24.367731349711462
50	24.192790344612163	26.76678614106588	24.83161828596739	24.208805228354567
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	23.0
1	25.0
2	27.0
3	81.0
4	135.0
5	172.0
6	209.0
7	231.0
8	253.0
9	362.0
10	471.0
11	782.5
12	1094.0
13	2124.5
14	3155.0
15	4594.0
16	6033.0
17	6879.0
18	7725.0
19	8166.5
20	8608.0
21	9317.0
22	10026.0
23	11862.0
24	13698.0
25	16336.5
26	18975.0
27	25914.0
28	32853.0
29	40702.0
30	48551.0
31	57236.5
32	65922.0
33	78978.5
34	92035.0
35	108530.5
36	125026.0
37	135163.5
38	145301.0
39	146786.5
40	148272.0
41	152306.0
42	156340.0
43	153953.0
44	151566.0
45	162553.5
46	173541.0
47	183314.0
48	193087.0
49	195040.0
50	196993.0
51	187781.0
52	178569.0
53	175051.0
54	171533.0
55	172921.5
56	174310.0
57	169205.0
58	164100.0
59	147841.5
60	131583.0
61	116474.0
62	101365.0
63	88191.5
64	75018.0
65	63807.5
66	52597.0
67	45842.5
68	39088.0
69	35963.5
70	32839.0
71	25290.5
72	17742.0
73	15087.0
74	12432.0
75	9738.5
76	7045.0
77	6222.0
78	5399.0
79	4450.0
80	3501.0
81	2864.5
82	2228.0
83	1886.0
84	1544.0
85	1118.0
86	692.0
87	498.5
88	305.0
89	252.5
90	200.0
91	171.5
92	143.0
93	118.5
94	94.0
95	94.0
96	94.0
97	73.0
98	52.0
99	58.0
100	64.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005879263728621843
2	3.606910262958186E-4
3	3.967601289254005E-4
4	0.0
5	0.0
6	1.0820730788874559E-4
7	2.52483718407073E-4
8	0.0
9	8.295893604803827E-4
10	0.001009934873628292
11	3.606910262958186E-5
12	3.606910262958186E-5
13	3.967601289254005E-4
14	3.2462192366623674E-4
15	0.003282288339291949
16	6.492438473324735E-4
17	0.0
18	2.8855282103665487E-4
19	3.606910262958186E-5
20	7.213820525916372E-4
21	0.0
22	1.4427641051832743E-4
23	6.853129499620553E-4
24	3.2462192366623674E-4
25	0.0016591787209607653
26	0.00504967436814146
27	0.005879263728621843
28	0.0038954630839948406
29	0.00252483718407073
30	0.003534772057699022
31	0.006420300268065572
32	0.003462633852439859
33	0.0046529142392160595
34	0.002849459107736967
35	0.00252483718407073
36	0.006961336807509298
37	0.003859393981365259
38	0.007610580654841772
39	0.004147946802401914
40	0.002019869747256584
41	0.0033544265445511127
42	0.0019116624393678385
43	0.0014066950025536924
44	0.0016952478235903474
45	0.001514902310442438
46	0.0011542112841466195
47	0.002452698978811566
48	0.0019477315419974203
49	6.853129499620553E-4
50	0.001262418592035365
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2772456.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.78630150275444
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.40760898490124	31.085101025830753
2	15.384473352673297	13.780273240678357
3	6.2737585800930615	8.429353299706213
4	3.0971923067848652	5.548471538547141
5	1.780707262937141	3.9875646183023714
6	1.0356517294935201	2.7829806365367524
7	0.7000189871339916	2.194588299080501
8	0.44522386308049766	1.5951944134515383
9	0.3334487171332224	1.344054130311166
>10	1.3572275069305493	10.335475939567454
>50	0.08634270465146412	2.685841311709547
>100	0.07892355726684615	7.548815776078987
>500	0.012948297947005763	3.9771066576941183
>1k	0.006474148973488062	4.705179112505002
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	3181	0.1147358154646999	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	3056	0.11022717763600215	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2923	0.10542998698626777	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2858	0.10308549531534494	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.606910262958186E-5	0.0
5	0.0	0.0	0.0	3.606910262958186E-5	0.0
6	0.0	0.0	0.0	3.606910262958186E-5	0.0
7	0.0	0.0	0.0	1.0820730788874558E-4	0.0
8	0.0	0.0	0.0	1.0820730788874558E-4	0.0
9	0.0	0.0	0.0	1.8034551314790928E-4	0.0
10	0.0	0.0	0.0	1.8034551314790928E-4	0.0
11	0.0	0.0	0.0	1.8034551314790928E-4	0.0
12	0.0	0.0	0.0	2.52483718407073E-4	1.4427641051832743E-4
13	0.0	0.0	0.0	2.52483718407073E-4	1.4427641051832743E-4
14	0.0	0.0	0.0	2.52483718407073E-4	1.4427641051832743E-4
15	0.0	0.0	0.0	2.52483718407073E-4	2.1641461577749115E-4
16	0.0	0.0	0.0	2.52483718407073E-4	2.1641461577749115E-4
17	3.606910262958186E-5	0.0	0.0	2.52483718407073E-4	2.1641461577749115E-4
18	3.606910262958186E-5	0.0	0.0	2.8855282103665487E-4	2.1641461577749115E-4
19	3.606910262958186E-5	0.0	0.0	2.8855282103665487E-4	2.1641461577749115E-4
20	3.606910262958186E-5	0.0	0.0	2.8855282103665487E-4	2.52483718407073E-4
21	3.606910262958186E-5	0.0	0.0	2.8855282103665487E-4	2.8855282103665487E-4
22	3.606910262958186E-5	0.0	0.0	3.2462192366623674E-4	2.8855282103665487E-4
23	3.606910262958186E-5	0.0	0.0	4.6889833418456417E-4	2.8855282103665487E-4
24	3.606910262958186E-5	0.0	0.0	7.57451155221219E-4	2.8855282103665487E-4
25	3.606910262958186E-5	0.0	0.0	8.656584631099646E-4	2.8855282103665487E-4
26	3.606910262958186E-5	0.0	0.0	0.0010820730788874558	3.9676012892540043E-4
27	3.606910262958186E-5	0.0	0.0	0.0011902803867762013	3.9676012892540043E-4
28	3.606910262958186E-5	0.0	0.0	0.0014427641051832742	3.9676012892540043E-4
29	3.606910262958186E-5	0.0	0.0	0.002019869747256584	3.9676012892540043E-4
30	7.213820525916372E-5	0.0	0.0	0.0037151175708469313	3.9676012892540043E-4
31	7.213820525916372E-5	0.0	0.0	0.009450104888950447	3.9676012892540043E-4
32	7.213820525916372E-5	0.0	0.0	0.015653990541238526	3.9676012892540043E-4
33	7.213820525916372E-5	0.0	0.0	0.021280770551453297	3.9676012892540043E-4
34	7.213820525916372E-5	0.0	0.0	0.028999558514183815	3.9676012892540043E-4
35	7.213820525916372E-5	0.0	0.0	0.03689869199006224	3.9676012892540043E-4
36	7.213820525916372E-5	0.0	0.0	0.04948680880778631	3.9676012892540043E-4
37	7.213820525916372E-5	0.0	0.0	0.07022654281979587	3.9676012892540043E-4
38	7.213820525916372E-5	0.0	0.0	0.10128204018386586	4.6889833418456417E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6105	0.0	21.728786	1
TTAGGAC	1800	0.0	19.065496	3
TGTAGGA	3055	0.0	18.86593	2
GATATAC	1135	0.0	18.800945	1
TAGGACG	2880	0.0	18.713789	4
GTAGGAC	3080	0.0	18.070316	3
AGGACGT	3075	0.0	18.027836	5
GACGTGA	1625	0.0	18.004723	7
ACCGTCG	820	0.0	17.97418	8
GTCCTAC	2430	0.0	17.834616	1
ATACACT	1215	0.0	17.743446	4
GGACGTG	3060	0.0	17.397308	6
CTGTAGG	2985	0.0	17.39288	1
GTCCTAA	1600	0.0	17.186754	1
AACGCAG	7750	0.0	17.144442	6
CGTCGTA	860	0.0	17.13879	10
TTTAGGA	2110	0.0	16.99391	2
CCGTCGT	855	0.0	16.98172	9
GCGAGAA	1705	0.0	16.903101	20
ATACCGT	885	0.0	16.902609	6
>>END_MODULE