FastQCFastQC Report
Thu 2 Feb 2017
SRR4063868_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063868_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2194091
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47430.2161715261582131No Hit
TATCAACGCAGAGTACTTTTTTTTT34010.15500724445795547No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22170.10104412260020208No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA1155.30963E-912.39414715
ACCGAGT658.019506E-411.6933888
CGCCAGT3000.011.40261418
AGGCCCG3300.011.22831110
GTATCAA37150.011.1703931
CGAGCCG3150.011.16128915
CGCATCG3100.011.03378113
GTCCTAA5550.010.9504731
CGGTCCA2950.010.95016610
GCTCGTA2000.010.926019
ACCGTCG3600.010.8204448
CGTCGTA3250.010.81638410
ACCGGCG1855.456968E-1210.78478058
CGTGCGC1158.8104025E-710.74012410
TCGCGTA1158.8104025E-710.7401249
TCGCCAG3100.010.72826717
CCGTCGT3400.010.61862859
CGGTTCT4150.010.53181412
ACTCTAA2900.010.48372810
ATAAGAC1451.4528268E-810.4787083
Produced by FastQC (version 0.11.5)