##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063868_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2194091
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.20382427164598	32.0	32.0	32.0	32.0	32.0
2	30.730134711823712	32.0	32.0	32.0	32.0	32.0
3	30.760591971800622	32.0	32.0	32.0	32.0	32.0
4	30.7909015624238	32.0	32.0	32.0	32.0	32.0
5	30.674586879030997	32.0	32.0	32.0	32.0	32.0
6	34.342992154837695	36.0	36.0	36.0	32.0	36.0
7	34.275763402702985	36.0	36.0	36.0	32.0	36.0
8	34.229145463884585	36.0	36.0	36.0	32.0	36.0
9	34.38730708981533	36.0	36.0	36.0	32.0	36.0
10	34.036039070394075	36.0	36.0	36.0	32.0	36.0
11	34.37908409450657	36.0	36.0	36.0	32.0	36.0
12	34.163608984312866	36.0	36.0	36.0	32.0	36.0
13	34.257508918271846	36.0	36.0	36.0	32.0	36.0
14	34.1496783861745	36.0	36.0	36.0	32.0	36.0
15	34.068851291947325	36.0	36.0	36.0	32.0	36.0
16	34.08022684565043	36.0	36.0	36.0	32.0	36.0
17	33.997333747779834	36.0	36.0	36.0	32.0	36.0
18	33.99785560398361	36.0	36.0	36.0	32.0	36.0
19	34.01313072247231	36.0	36.0	36.0	32.0	36.0
20	33.98304628203662	36.0	36.0	36.0	32.0	36.0
21	33.97404027453738	36.0	36.0	36.0	32.0	36.0
22	33.950002529521335	36.0	36.0	36.0	32.0	36.0
23	33.89071556284584	36.0	36.0	36.0	32.0	36.0
24	33.8727267920975	36.0	36.0	36.0	32.0	36.0
25	33.462525483218336	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	6.0
4	21.0
5	32.0
6	87.0
7	37.0
8	128.0
9	133.0
10	161.0
11	74.0
12	123.0
13	98.0
14	199.0
15	348.0
16	608.0
17	889.0
18	1194.0
19	1658.0
20	2577.0
21	3925.0
22	6061.0
23	9332.0
24	13839.0
25	19704.0
26	27664.0
27	36080.0
28	47863.0
29	64191.0
30	83389.0
31	111949.0
32	155534.0
33	214983.0
34	447263.0
35	943940.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.580705021964974	17.799992248998638	11.929930947696699	25.68937178133969
2	16.216109043589633	20.08833241795181	38.62337253901006	25.072185999448493
3	18.41966490143074	24.80628210685215	28.655344179985697	28.118708811731413
4	12.40032434043713	16.374041643744206	36.48515422351322	34.74047979230544
5	14.367350585288055	37.091915362620604	33.725318237986905	14.815415814104437
6	33.00677548281697	35.88020195168913	17.87284300425929	13.24017956123461
7	29.862353995581685	30.57948630514798	21.398268962681403	18.15989073658893
8	27.661625171941672	33.418394214097184	19.67856196807504	19.241418645886096
9	27.534323343704013	14.695165297867208	18.890526898493917	38.87998445993486
10	15.998186864251037	27.15745369344325	31.652124187030246	25.19223525527547
11	36.21990870578364	21.231605492245464	23.433246234455588	19.11523956751531
12	25.08017483404083	23.70425063437539	29.325838187300818	21.88973634428296
13	29.449115439762707	20.2518506627361	25.39384729888978	24.905186598611408
14	23.5041883319598	20.296318728253944	25.74938236526852	30.45011057451774
15	24.713461135922007	28.155575793422955	22.520292465052815	24.61067060560222
16	25.039278721809964	26.02164582519065	24.80736893904809	24.1317065139513
17	23.662167739522836	26.296491384087066	25.768158176167315	24.27318270022278
18	24.076546364362407	25.397215625084097	27.113378692047114	23.41285931850638
19	25.468166331431384	25.001436722630434	25.654392950480958	23.87600399545722
20	25.64282909113798	24.769120667548812	25.385346238036398	24.202704003276807
21	26.533997666562982	24.463095115381073	24.69393023588877	24.308976982167177
22	25.58499657453957	24.986704104987854	25.120803354853074	24.30749596561951
23	24.13770768180939	24.932627710856565	25.74611275119783	25.183551856136216
24	24.61658736298221	25.30687606731569	25.720620539167747	24.355916030534353
25	24.544390951448076	25.031939956075433	25.726385984277467	24.697283108199024
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	187.0
1	187.0
2	525.5
3	864.0
4	864.0
5	864.0
6	2128.0
7	3392.0
8	3392.0
9	3392.0
10	3498.0
11	3604.0
12	3604.0
13	3604.0
14	4613.0
15	5622.0
16	5622.0
17	5622.0
18	10272.0
19	14922.0
20	14922.0
21	14922.0
22	24797.5
23	34673.0
24	34673.0
25	34673.0
26	52761.5
27	70850.0
28	70850.0
29	70850.0
30	91216.0
31	111582.0
32	111582.0
33	111582.0
34	133126.5
35	154671.0
36	154671.0
37	154671.0
38	175180.0
39	195689.0
40	195689.0
41	195689.0
42	219062.5
43	242436.0
44	242436.0
45	242436.0
46	265427.5
47	288419.0
48	288419.0
49	288419.0
50	289671.0
51	290923.0
52	290923.0
53	290923.0
54	267335.5
55	243748.0
56	243748.0
57	243748.0
58	221486.0
59	199224.0
60	199224.0
61	199224.0
62	172488.5
63	145753.0
64	145753.0
65	145753.0
66	118714.5
67	91676.0
68	91676.0
69	91676.0
70	68828.5
71	45981.0
72	45981.0
73	45981.0
74	34977.5
75	23974.0
76	23974.0
77	23974.0
78	19150.0
79	14326.0
80	14326.0
81	14326.0
82	9909.5
83	5493.0
84	5493.0
85	5493.0
86	4219.5
87	2946.0
88	2946.0
89	2946.0
90	2194.0
91	1442.0
92	1442.0
93	1442.0
94	1012.5
95	583.0
96	583.0
97	583.0
98	847.0
99	1111.0
100	1111.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03764656980954755
2	0.0048311578690218415
3	0.0022788480514253965
4	0.004557696102850793
5	0.009753469660100697
6	0.013627511347523873
7	0.02246944178705441
8	0.034638490381666026
9	0.04785580907993333
10	0.05492023803935205
11	0.05382639097466787
12	0.06303293710242647
13	0.06212139788185631
14	0.06617774741339352
15	0.05842966403854717
16	0.06508390034870933
17	0.06134658954437168
18	0.07483737000881002
19	0.0729687146066412
20	0.07802775728080558
21	0.07369794598309733
22	0.07679717933303587
23	0.08181064504617173
24	0.07634140972275079
25	0.07729852590434946
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2194091.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.33866053333366
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.59026433091364	37.04435334712154
2	14.877419453126326	14.978187349258814
3	5.104827592281821	7.709105497472088
4	2.3256665335285005	4.682837525801041
5	1.1584920173510636	2.9158468196006035
6	0.6987277417437022	2.1103811158115438
7	0.44286601468486914	1.5605297382480392
8	0.3219905320381696	1.2966857669773533
9	0.22886738409430923	1.0368789799567968
>10	1.0820792183293537	9.730945603814884
>50	0.08319243403006973	2.9338998794641507
>100	0.07249598354299683	7.830192774819539
>500	0.009104697455143992	3.143100894372598
>1k	0.004006066880263338	3.027054707281038
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4743	0.2161715261582131	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3401	0.15500724445795547	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2217	0.10104412260020208	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.557696102850793E-5
2	0.0	0.0	0.0	0.0	4.557696102850793E-5
3	0.0	0.0	0.0	0.0	4.557696102850793E-5
4	0.0	0.0	0.0	0.0	4.557696102850793E-5
5	0.0	0.0	0.0	0.0	9.115392205701586E-5
6	0.0	0.0	0.0	0.0	1.367308830855238E-4
7	0.0	0.0	0.0	4.557696102850793E-5	1.367308830855238E-4
8	0.0	0.0	0.0	4.557696102850793E-5	1.367308830855238E-4
9	0.0	0.0	0.0	4.557696102850793E-5	1.8230784411403173E-4
10	0.0	0.0	0.0	9.115392205701586E-5	1.8230784411403173E-4
11	0.0	0.0	0.0	9.115392205701586E-5	1.8230784411403173E-4
12	0.0	0.0	0.0	9.115392205701586E-5	2.2788480514253966E-4
13	0.0	0.0	0.0	9.115392205701586E-5	2.734617661710476E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTTA	115	5.30963E-9	12.394147	15
ACCGAGT	65	8.019506E-4	11.693388	8
CGCCAGT	300	0.0	11.402614	18
AGGCCCG	330	0.0	11.228311	10
GTATCAA	3715	0.0	11.170393	1
CGAGCCG	315	0.0	11.161289	15
CGCATCG	310	0.0	11.033781	13
GTCCTAA	555	0.0	10.950473	1
CGGTCCA	295	0.0	10.950166	10
GCTCGTA	200	0.0	10.92601	9
ACCGTCG	360	0.0	10.820444	8
CGTCGTA	325	0.0	10.816384	10
ACCGGCG	185	5.456968E-12	10.7847805	8
CGTGCGC	115	8.8104025E-7	10.740124	10
TCGCGTA	115	8.8104025E-7	10.740124	9
TCGCCAG	310	0.0	10.728267	17
CCGTCGT	340	0.0	10.6186285	9
CGGTTCT	415	0.0	10.531814	12
ACTCTAA	290	0.0	10.483728	10
ATAAGAC	145	1.4528268E-8	10.478708	3
>>END_MODULE