FastQCFastQC Report
Thu 2 Feb 2017
SRR4063868_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063868_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2194091
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG10900.021.7966964
GTATCAA42050.020.6135771
TTAGGAC8900.019.2796023
GACGTGA7850.019.0560427
TACCGTA708.129886E-418.8558737
AGGACGT12500.018.4787565
GTAGGAC12500.018.126783
TAACGGC1601.1023076E-917.87664836
GGACGTG12550.017.7039876
GTCCTAC13100.017.2977261
ACCGTCG5100.017.253748
CGTGAAA9450.016.9943399
CGTCGTA5050.016.98972910
TGTAGGA15050.016.663332
CCGTCGT5300.016.603419
TCGTCGA800.001989335516.4996419
AACGCAG52400.016.3309616
GTCCTAA8600.016.1162971
GGCGTTA4950.015.99928742
ACGTGAA10050.015.9790238
Produced by FastQC (version 0.11.5)