##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063868_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2194091
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.311683517228776	32.0	32.0	32.0	32.0	32.0
2	31.385461678663283	32.0	32.0	32.0	32.0	32.0
3	31.474450695071443	32.0	32.0	32.0	32.0	32.0
4	31.565088685929616	32.0	32.0	32.0	32.0	32.0
5	31.512568074888417	32.0	32.0	32.0	32.0	32.0
6	35.093532127883485	36.0	36.0	36.0	36.0	36.0
7	35.11654028934989	36.0	36.0	36.0	36.0	36.0
8	35.062627302149274	36.0	36.0	36.0	36.0	36.0
9	35.172039810563916	36.0	36.0	36.0	36.0	36.0
10	35.02873217200198	36.0	36.0	36.0	36.0	36.0
11	35.174666866597605	36.0	36.0	36.0	36.0	36.0
12	35.08020360140031	36.0	36.0	36.0	36.0	36.0
13	35.12102415077588	36.0	36.0	36.0	36.0	36.0
14	35.066711453627036	36.0	36.0	36.0	36.0	36.0
15	35.037030825066054	36.0	36.0	36.0	36.0	36.0
16	35.04835806719047	36.0	36.0	36.0	36.0	36.0
17	35.021377417800814	36.0	36.0	36.0	36.0	36.0
18	35.01783107446318	36.0	36.0	36.0	36.0	36.0
19	35.016408161739875	36.0	36.0	36.0	36.0	36.0
20	35.007395773466094	36.0	36.0	36.0	36.0	36.0
21	34.99796954638618	36.0	36.0	36.0	36.0	36.0
22	34.98009335073158	36.0	36.0	36.0	36.0	36.0
23	34.936200914182685	36.0	36.0	36.0	36.0	36.0
24	34.9066000453035	36.0	36.0	36.0	32.0	36.0
25	34.88392596296143	36.0	36.0	36.0	32.0	36.0
26	34.8226213042212	36.0	36.0	36.0	32.0	36.0
27	34.81759781157664	36.0	36.0	36.0	32.0	36.0
28	34.78891349538374	36.0	36.0	36.0	32.0	36.0
29	34.76328876058468	36.0	36.0	36.0	32.0	36.0
30	34.73493989082495	36.0	36.0	36.0	32.0	36.0
31	34.730234981137976	36.0	36.0	36.0	32.0	36.0
32	34.690252592075716	36.0	36.0	36.0	32.0	36.0
33	34.67158654768649	36.0	36.0	36.0	32.0	36.0
34	34.66008975926705	36.0	36.0	36.0	32.0	36.0
35	34.623499207644535	36.0	36.0	36.0	32.0	36.0
36	34.598281475107456	36.0	36.0	36.0	32.0	36.0
37	34.57657544741763	36.0	36.0	36.0	32.0	36.0
38	34.540850402285045	36.0	36.0	36.0	32.0	36.0
39	34.53012751066387	36.0	36.0	36.0	32.0	36.0
40	34.511858897374815	36.0	36.0	36.0	32.0	36.0
41	34.493928009366975	36.0	36.0	36.0	32.0	36.0
42	34.43546233952922	36.0	36.0	36.0	32.0	36.0
43	34.41943292233549	36.0	36.0	36.0	32.0	36.0
44	34.35904117012467	36.0	36.0	36.0	32.0	36.0
45	34.32361647716526	36.0	36.0	36.0	32.0	36.0
46	34.28649723279481	36.0	36.0	36.0	32.0	36.0
47	34.26490605904677	36.0	36.0	36.0	32.0	36.0
48	34.2141588475592	36.0	36.0	36.0	32.0	36.0
49	34.19422075018766	36.0	36.0	36.0	32.0	36.0
50	33.60572920630913	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	5.0
19	9.0
20	31.0
21	72.0
22	254.0
23	717.0
24	1830.0
25	4088.0
26	8192.0
27	15013.0
28	24750.0
29	38468.0
30	55004.0
31	78413.0
32	113234.0
33	182397.0
34	380631.0
35	1290980.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.776646863254356	18.612168842854135	12.528978101990157	26.082206191901356
2	15.491279740174269	20.896947521655942	38.0344708457189	25.577301892450887
3	17.679086124323405	25.436172908603318	28.991232787805753	27.893508179267524
4	12.097629496679946	17.012785704877327	36.56279525325066	34.326789545192064
5	14.043127655142836	37.4867769841816	33.49879289418716	14.971302466488401
6	32.98397009781289	36.34158536101805	17.606913490668326	13.067531050500733
7	29.700253727173326	30.72933686828565	21.404829065730695	18.165580338810322
8	27.62990579237862	33.640415844381955	19.51879822614387	19.21088013709556
9	27.642635114809988	14.419751510897605	18.801673609655158	39.13593976463724
10	15.867116121392884	27.309388140557306	31.740613090034664	25.082882648015147
11	36.41982032650423	21.173187438442618	23.386131204220792	19.020861030832357
12	24.977519143480507	23.703322882526642	29.393292614839233	21.92586535915362
13	29.460325794420722	20.24577010076647	25.395575281336214	24.898328823476596
14	23.432203795389693	20.242397393352938	25.751623799099672	30.5737750121577
15	24.73674638910805	28.270404519261056	22.447717329227565	24.545131762403333
16	25.075909293521708	25.984952139087884	24.704863025998307	24.2342755413921
17	23.62171851577715	26.348405786268664	25.661606560530075	24.368269137424107
18	24.092071098365743	25.402684579052696	27.074131518030054	23.431112804551507
19	25.471641786963257	24.88661591520133	25.789085320526816	23.852656977308598
20	25.692101332723205	24.727505253912916	25.424152913363645	24.15624050000023
21	26.539236522095027	24.350995469194302	24.78611871613347	24.323649292577198
22	25.60262997295919	24.922986330102077	25.223885426812288	24.250498270126446
23	24.18314058687173	24.824185510607208	25.782129499734285	25.210544402786777
24	24.730912763595196	25.17114203467142	25.74678088897235	24.351164312761043
25	24.6404250771063	24.90231530836367	25.832148689477762	24.625110925052265
26	24.17348158263452	25.714848297281357	26.15988791054722	23.9517822095369
27	24.745117486617758	25.39440242995289	25.34016241011654	24.520317673312817
28	24.051331019441704	25.338799769370485	25.932511240909843	24.677357970277964
29	24.403802863314148	25.07945421008181	25.599045774649493	24.917697151954556
30	24.143277900384778	25.287716237268697	25.980422989587066	24.58858287275946
31	25.061750284992552	24.92354997932921	25.184954581235296	24.829745154442946
32	24.54129862079653	25.37387990993701	25.147765289286333	24.93705617998013
33	24.116716446346196	25.123370499831132	25.82929542876234	24.930617625060336
34	24.72142409171127	25.184684159094555	25.90304719746802	24.190844551726148
35	25.111165602564057	25.13596022650618	25.73212508158525	24.02074908934451
36	24.150154587790958	25.618818368575763	25.556645953163752	24.674381090469524
37	24.927666758431585	25.39844813975493	25.218686132983777	24.455198968829706
38	24.4838071397088	25.461700834906253	25.50641574657721	24.548076278807727
39	25.05569766106287	25.05004585257531	25.147402811683566	24.746853674678256
40	25.394958136397406	25.13643842609262	25.554709826210214	23.913893611299756
41	24.496926637660472	25.355854611684336	25.911047058040648	24.23617169261454
42	25.314156039074714	25.80051621360104	25.36976089456362	23.51556685276062
43	24.438870804691582	25.21715469691321	25.684234418597867	24.659740079797345
44	24.279257886528068	25.54308044728079	25.23971563194774	24.937946034243396
45	24.378502645329018	25.665616556733283	25.30450453406841	24.65137626386929
46	24.467631270219346	25.280598269328763	25.21820258497709	25.033567875474805
47	24.85026460355956	25.245517737394586	25.54783716044898	24.356380498596877
48	25.06407092262584	25.623765697546958	24.78878131020536	24.52338206962184
49	24.575630014639422	25.65726984844645	25.002233286358354	24.76486685055577
50	24.546489989075013	26.18264007296086	24.709476024508067	24.561393913456055
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	23.0
1	30.0
2	37.0
3	112.5
4	188.0
5	224.0
6	260.0
7	251.0
8	242.0
9	350.0
10	458.0
11	751.0
12	1044.0
13	1945.5
14	2847.0
15	3864.5
16	4882.0
17	5301.5
18	5721.0
19	5883.0
20	6045.0
21	6709.5
22	7374.0
23	8577.5
24	9781.0
25	11583.5
26	13386.0
27	17549.0
28	21712.0
29	26770.0
30	31828.0
31	37300.0
32	42772.0
33	50683.5
34	58595.0
35	67540.5
36	76486.0
37	86814.5
38	97143.0
39	102221.5
40	107300.0
41	113750.5
42	120201.0
43	122209.5
44	124218.0
45	134411.0
46	144604.0
47	152885.0
48	161166.0
49	164570.5
50	167975.0
51	162828.0
52	157681.0
53	154778.5
54	151876.0
55	151434.0
56	150992.0
57	145652.5
58	140313.0
59	126582.0
60	112851.0
61	98109.0
62	83367.0
63	71685.5
64	60004.0
65	50747.0
66	41490.0
67	35465.0
68	29440.0
69	26155.0
70	22870.0
71	17767.0
72	12664.0
73	10980.5
74	9297.0
75	7216.0
76	5135.0
77	4483.0
78	3831.0
79	3157.5
80	2484.0
81	1993.5
82	1503.0
83	1279.0
84	1055.0
85	755.0
86	455.0
87	321.5
88	188.0
89	138.0
90	88.0
91	80.0
92	72.0
93	59.0
94	46.0
95	45.5
96	45.0
97	39.5
98	34.0
99	28.0
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008796353478502031
2	2.2788480514253964E-4
3	3.190387271995555E-4
4	0.0
5	0.0
6	1.8230784411403173E-4
7	9.115392205701586E-5
8	4.557696102850793E-5
9	0.001412885791883746
10	0.0011394240257126982
11	0.0
12	9.115392205701586E-5
13	3.6461568822806345E-4
14	3.6461568822806345E-4
15	0.004740003946964826
16	0.0011394240257126982
17	0.0
18	1.8230784411403173E-4
19	0.0
20	5.469235323420953E-4
21	0.0
22	0.0
23	7.748083374846348E-4
24	3.190387271995555E-4
25	0.0019142323631973332
26	0.005879427972677523
27	0.006335197582962603
28	0.004375388258736762
29	0.002734617661710476
30	0.0040563495315372065
31	0.007930391218960381
32	0.003235964233024063
33	0.004101926492565714
34	0.0034182720771380945
35	0.003053656388910032
36	0.008750776517473524
37	0.0036005799212521267
38	0.008750776517473524
39	0.004694426985936317
40	0.002780194622738984
41	0.0035094259991951112
42	0.0017319245190833014
43	0.0015040397139407617
44	0.0015040397139407617
45	0.0015951936359977774
46	0.0013217318698267302
47	0.0024611558955394282
48	0.0017319245190833014
49	6.83654415427619E-4
50	0.0017319245190833014
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2194091.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.17817264005923
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.99996353145174	39.35182836033628
2	14.774112413193588	15.713206010249014
3	5.055524235208302	8.065306216977314
4	2.227746247909913	4.738698982783901
5	1.1460881288965856	3.047343618959255
6	0.7021597073688056	2.240374208361109
7	0.4503599674116198	1.6764524068030597
8	0.30673054184817516	1.3049095766704937
9	0.21423744065377326	1.0253480044545717
>10	0.9839443946581005	9.096715529114734
>50	0.06976861676271234	2.586283322392995
>100	0.059200983777318875	6.6069898180864355
>500	0.007924311517410182	2.925343296184614
>1k	0.0022394793418767565	1.62120064862614
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	9.115392205701586E-5
7	0.0	0.0	0.0	0.0	9.115392205701586E-5
8	0.0	0.0	0.0	0.0	1.367308830855238E-4
9	0.0	0.0	0.0	0.0	1.367308830855238E-4
10	0.0	0.0	0.0	4.557696102850793E-5	1.367308830855238E-4
11	0.0	0.0	0.0	4.557696102850793E-5	1.367308830855238E-4
12	0.0	0.0	0.0	4.557696102850793E-5	1.8230784411403173E-4
13	0.0	0.0	0.0	4.557696102850793E-5	2.734617661710476E-4
14	0.0	0.0	0.0	4.557696102850793E-5	2.734617661710476E-4
15	0.0	0.0	0.0	1.367308830855238E-4	3.6461568822806345E-4
16	0.0	0.0	0.0	1.8230784411403173E-4	3.6461568822806345E-4
17	0.0	0.0	0.0	2.2788480514253966E-4	3.6461568822806345E-4
18	4.557696102850793E-5	0.0	0.0	3.190387271995555E-4	3.6461568822806345E-4
19	4.557696102850793E-5	0.0	0.0	3.190387271995555E-4	3.6461568822806345E-4
20	4.557696102850793E-5	0.0	0.0	3.190387271995555E-4	3.6461568822806345E-4
21	4.557696102850793E-5	0.0	0.0	4.101926492565714E-4	3.6461568822806345E-4
22	4.557696102850793E-5	0.0	0.0	4.101926492565714E-4	3.6461568822806345E-4
23	4.557696102850793E-5	0.0	0.0	9.115392205701587E-4	3.6461568822806345E-4
24	4.557696102850793E-5	0.0	0.0	0.0018686554021688253	3.6461568822806345E-4
25	4.557696102850793E-5	0.0	0.0	0.0022332710903968885	3.6461568822806345E-4
26	4.557696102850793E-5	0.0	0.0	0.0024611558955394282	4.5576961028507933E-4
27	4.557696102850793E-5	0.0	0.0	0.0028713485447959997	4.5576961028507933E-4
28	4.557696102850793E-5	0.0	0.0	0.0036917338433091426	4.5576961028507933E-4
29	4.557696102850793E-5	0.0	0.0	0.004831157869021841	5.013465713135873E-4
30	4.557696102850793E-5	0.0	0.0	0.009069815244673079	5.013465713135873E-4
31	4.557696102850793E-5	0.0	0.0	0.019871555008429458	5.013465713135873E-4
32	4.557696102850793E-5	0.0	0.0	0.03167598791481301	5.469235323420952E-4
33	4.557696102850793E-5	0.0	0.0	0.041702919341084756	5.469235323420952E-4
34	4.557696102850793E-5	0.0	0.0	0.05255023606586965	6.38077454399111E-4
35	4.557696102850793E-5	0.0	0.0	0.06672467094573561	6.38077454399111E-4
36	4.557696102850793E-5	0.0	0.0	0.08741661125267822	6.38077454399111E-4
37	4.557696102850793E-5	0.0	0.0	0.11813548298589256	6.38077454399111E-4
38	4.557696102850793E-5	0.0	0.0	0.15938263271669223	6.83654415427619E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACG	1090	0.0	21.796696	4
GTATCAA	4205	0.0	20.613577	1
TTAGGAC	890	0.0	19.279602	3
GACGTGA	785	0.0	19.056042	7
TACCGTA	70	8.129886E-4	18.855873	7
AGGACGT	1250	0.0	18.478756	5
GTAGGAC	1250	0.0	18.12678	3
TAACGGC	160	1.1023076E-9	17.876648	36
GGACGTG	1255	0.0	17.703987	6
GTCCTAC	1310	0.0	17.297726	1
ACCGTCG	510	0.0	17.25374	8
CGTGAAA	945	0.0	16.994339	9
CGTCGTA	505	0.0	16.989729	10
TGTAGGA	1505	0.0	16.66333	2
CCGTCGT	530	0.0	16.60341	9
TCGTCGA	80	0.0019893355	16.499641	9
AACGCAG	5240	0.0	16.330961	6
GTCCTAA	860	0.0	16.116297	1
GGCGTTA	495	0.0	15.999287	42
ACGTGAA	1005	0.0	15.979023	8
>>END_MODULE