FastQCFastQC Report
Thu 2 Feb 2017
SRR4063867_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063867_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences316952
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT3840.12115399177162471No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTGTC453.5222827E-516.8901527
GGTATCA1450.015.0605771
GTTCAAA456.769844E-414.7695521
GTATTAA400.005286323414.2420681
GTCTTGA500.001501240813.2925981
GGACGTG604.0789146E-412.6656146
GTATCAA4400.012.2999681
AGTCCAG550.003057462212.09181310
ACTGTCC550.003057462212.0918138
CTATGGT802.851932E-511.8740134
AGGACGT657.9980807E-411.6913365
TAAAGTG752.0654839E-411.3990525
AGAACTG600.00586178911.0824135
CTTAGGG600.005882502511.0771643
CTCTAGG600.00588942111.0754172
TAGGACG700.001486758710.856244
TAGGATA856.567123E-410.0579874
CAGAACT850.0074126058.94043354
ACACCGT850.0074126058.94043356
ATGGTGT1107.108798E-48.6356456
Produced by FastQC (version 0.11.5)