##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063867_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	316952
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32910346046089	32.0	32.0	32.0	32.0	32.0
2	31.034841237789948	32.0	32.0	32.0	32.0	32.0
3	31.032818849541886	32.0	32.0	32.0	32.0	32.0
4	31.057898356848987	32.0	32.0	32.0	32.0	32.0
5	31.025562230243064	32.0	32.0	32.0	32.0	32.0
6	34.670019435119514	36.0	36.0	36.0	32.0	36.0
7	34.62762815820692	36.0	36.0	36.0	32.0	36.0
8	34.60654610161791	36.0	36.0	36.0	32.0	36.0
9	34.69484338322522	36.0	36.0	36.0	32.0	36.0
10	34.49103965269189	36.0	36.0	36.0	32.0	36.0
11	34.655752290567655	36.0	36.0	36.0	32.0	36.0
12	34.54500365986017	36.0	36.0	36.0	32.0	36.0
13	34.58901032332972	36.0	36.0	36.0	32.0	36.0
14	34.49038340190313	36.0	36.0	36.0	32.0	36.0
15	34.453750094651554	36.0	36.0	36.0	32.0	36.0
16	34.44956018576946	36.0	36.0	36.0	32.0	36.0
17	34.413652540447764	36.0	36.0	36.0	32.0	36.0
18	34.39317940886948	36.0	36.0	36.0	32.0	36.0
19	34.40475844922891	36.0	36.0	36.0	32.0	36.0
20	34.3920562104041	36.0	36.0	36.0	32.0	36.0
21	34.37656490572705	36.0	36.0	36.0	32.0	36.0
22	34.343487341931905	36.0	36.0	36.0	32.0	36.0
23	34.30728627678639	36.0	36.0	36.0	32.0	36.0
24	34.26170208738232	36.0	36.0	36.0	32.0	36.0
25	33.898388399505286	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	0.0
4	3.0
5	4.0
6	14.0
7	8.0
8	15.0
9	22.0
10	29.0
11	8.0
12	14.0
13	17.0
14	26.0
15	39.0
16	89.0
17	121.0
18	125.0
19	164.0
20	235.0
21	340.0
22	545.0
23	780.0
24	1296.0
25	1906.0
26	2873.0
27	3868.0
28	5426.0
29	7418.0
30	10098.0
31	13428.0
32	19058.0
33	27370.0
34	62131.0
35	159481.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.35624376633378	17.092554950951307	12.426616334414888	26.124584948300022
2	16.38269945541399	20.070802491339	37.46347866144167	26.08301939180534
3	19.09082877948433	23.256811842952157	28.569039716294153	29.083319661269357
4	13.255779466840831	16.750436201059504	34.78786272523908	35.205921606860585
5	14.930614717631247	36.166802345026916	33.628985946246125	15.273596991095713
6	33.74196927815012	34.468047509056255	17.815264998043595	13.974718214750023
7	30.574691605419034	30.376826778337744	20.194835317767126	18.853646298476093
8	28.49743549277993	32.59654383334649	19.27404718693285	19.63197348694074
9	28.14370312381626	15.000063132907396	17.975201393974597	38.88103234930175
10	15.894002102531562	27.53306120387295	32.494530576680845	24.078406116914643
11	36.11978369232032	21.59667393795518	22.744190598255525	19.53935177146898
12	25.303627747803493	23.623454237213224	28.588205952271963	22.484712062711324
13	29.957030596362284	20.63194385247066	24.539917976112672	24.87110757505438
14	24.382535778669503	19.73201910721446	25.35178806525246	30.533657048863578
15	24.85335808408943	26.912658875229674	22.80636953131413	25.42761350936677
16	25.556600914303036	26.050690778672998	23.742517616750437	24.650190690273533
17	23.8422074539456	25.89780422737533	25.280588486006096	24.97939983267297
18	23.89941872764185	26.20303802424231	25.774266779069144	24.123276469046694
19	25.7687171051593	25.378478131146814	25.367743402088223	23.485061361605663
20	25.872238044418623	24.823342195166617	24.932273281256904	24.372146479157852
21	26.3256768490015	24.646301997000553	24.759649538242957	24.26837161575499
22	26.105873363686307	24.861548759464256	24.35447306436641	24.678104812483028
23	25.06615051373215	25.02352369104079	24.99636882621518	24.91395696901188
24	25.23388387740475	25.47826608612736	24.681024384545193	24.606825651922694
25	25.105852264654345	24.961558498965946	24.91577601313484	25.01681322324487
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13.0
1	13.0
2	28.5
3	44.0
4	44.0
5	44.0
6	104.0
7	164.0
8	164.0
9	164.0
10	212.0
11	260.0
12	260.0
13	260.0
14	417.0
15	574.0
16	574.0
17	574.0
18	1160.0
19	1746.0
20	1746.0
21	1746.0
22	2584.5
23	3423.0
24	3423.0
25	3423.0
26	5525.0
27	7627.0
28	7627.0
29	7627.0
30	10284.0
31	12941.0
32	12941.0
33	12941.0
34	16169.0
35	19397.0
36	19397.0
37	19397.0
38	23547.0
39	27697.0
40	27697.0
41	27697.0
42	31845.0
43	35993.0
44	35993.0
45	35993.0
46	39713.5
47	43434.0
48	43434.0
49	43434.0
50	43766.5
51	44099.0
52	44099.0
53	44099.0
54	42652.0
55	41205.0
56	41205.0
57	41205.0
58	37043.5
59	32882.0
60	32882.0
61	32882.0
62	27449.5
63	22017.0
64	22017.0
65	22017.0
66	17530.5
67	13044.0
68	13044.0
69	13044.0
70	9577.0
71	6110.0
72	6110.0
73	6110.0
74	4257.0
75	2404.0
76	2404.0
77	2404.0
78	1791.0
79	1178.0
80	1178.0
81	1178.0
82	770.0
83	362.0
84	362.0
85	362.0
86	227.0
87	92.0
88	92.0
89	92.0
90	66.0
91	40.0
92	40.0
93	40.0
94	31.0
95	22.0
96	22.0
97	22.0
98	103.0
99	184.0
100	184.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.039122643176253816
2	0.004417072616673818
3	0.0012620207476210909
4	0.0034705570559579995
5	0.008203134859537092
6	0.013882228223831998
7	0.021769857896463816
8	0.04006915873696963
9	0.050480829904843635
10	0.060261490698907086
11	0.05710643882985436
12	0.06278553219414927
13	0.06783361518463364
14	0.06720260481082309
15	0.06120800625962291
16	0.06562507887629673
17	0.06530957368939146
18	0.07351270854892855
19	0.07035765667987581
20	0.0750902344834549
21	0.0716196774274969
22	0.07382821373583381
23	0.07887629672631818
24	0.07414371892273909
25	0.07477472929654963
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	316952.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.17090460802274
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.58482041372922	22.16478602688285
2	16.799229577668186	13.160820385502289
3	8.807695128573576	10.350161570937063
4	5.16895698687903	8.098908842240442
5	3.3290932584668274	6.520179722930786
6	2.220282623609321	5.218228731135071
7	1.6378014267709695	4.490791441845042
8	1.1581183185002921	3.629163374302291
9	0.8191115324134224	2.8876805729547743
>10	3.379666313353244	20.30180237170669
>50	0.07585674138454808	2.0063118048492457
>100	0.01936767865137398	1.1711651547134796
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	384	0.12115399177162471	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACTGTC	45	3.5222827E-5	16.890152	7
GGTATCA	145	0.0	15.060577	1
GTTCAAA	45	6.769844E-4	14.769552	1
GTATTAA	40	0.0052863234	14.242068	1
GTCTTGA	50	0.0015012408	13.292598	1
GGACGTG	60	4.0789146E-4	12.665614	6
GTATCAA	440	0.0	12.299968	1
AGTCCAG	55	0.0030574622	12.091813	10
ACTGTCC	55	0.0030574622	12.091813	8
CTATGGT	80	2.851932E-5	11.874013	4
AGGACGT	65	7.9980807E-4	11.691336	5
TAAAGTG	75	2.0654839E-4	11.399052	5
AGAACTG	60	0.005861789	11.082413	5
CTTAGGG	60	0.0058825025	11.077164	3
CTCTAGG	60	0.005889421	11.075417	2
TAGGACG	70	0.0014867587	10.85624	4
TAGGATA	85	6.567123E-4	10.057987	4
CAGAACT	85	0.007412605	8.9404335	4
ACACCGT	85	0.007412605	8.9404335	6
ATGGTGT	110	7.108798E-4	8.635645	6
>>END_MODULE