FastQCFastQC Report
Thu 2 Feb 2017
SRR4063867_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063867_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences316952
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA3950.033.4152071
AAGAGCG353.210995E-431.4311629
GGTATCA1100.029.9977441
TAAGGGT454.0622755E-529.3311234
CTTTACG300.00574081529.3311232
TACACCG300.00574081529.3311235
ATTAGAG500.00257740521.9983443
TTTGGAC803.6039783E-621.9983443
TATCAAC5900.021.9983432
TTAGGGT602.8677675E-421.9983434
ATCAACG6300.020.6016243
TCAACGC6350.020.4394054
CAACGCA6550.020.1511555
CCTTTAC550.004478392719.9984951
TATTAGA550.004478392719.9984952
AACGCAG6850.019.2686216
CCTTAGG808.967123E-519.248552
GCGGTGC600.007396825618.33484513
GAGCGGT600.007396825618.33484511
GTAATAT851.4274423E-418.1162823
Produced by FastQC (version 0.11.5)