##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063867_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	316952
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.375179837956537	32.0	32.0	32.0	32.0	32.0
2	31.484562331204724	32.0	32.0	32.0	32.0	32.0
3	31.512411974052853	32.0	32.0	32.0	32.0	32.0
4	31.58026136449683	32.0	32.0	32.0	32.0	32.0
5	31.55699285696257	32.0	32.0	32.0	32.0	32.0
6	35.14202150483354	36.0	36.0	36.0	36.0	36.0
7	35.14215401701204	36.0	36.0	36.0	36.0	36.0
8	35.11549382871854	36.0	36.0	36.0	36.0	36.0
9	35.19452156793458	36.0	36.0	36.0	36.0	36.0
10	35.07380612837275	36.0	36.0	36.0	36.0	36.0
11	35.18039009061309	36.0	36.0	36.0	36.0	36.0
12	35.12743885509478	36.0	36.0	36.0	36.0	36.0
13	35.12921199424518	36.0	36.0	36.0	36.0	36.0
14	35.087035260859686	36.0	36.0	36.0	36.0	36.0
15	35.08067467629168	36.0	36.0	36.0	36.0	36.0
16	35.093275953456676	36.0	36.0	36.0	36.0	36.0
17	35.061864257048384	36.0	36.0	36.0	36.0	36.0
18	35.0514746712436	36.0	36.0	36.0	36.0	36.0
19	35.03259168580731	36.0	36.0	36.0	36.0	36.0
20	35.045221358439136	36.0	36.0	36.0	36.0	36.0
21	35.043836290668615	36.0	36.0	36.0	36.0	36.0
22	35.0154250485878	36.0	36.0	36.0	36.0	36.0
23	34.96783109114314	36.0	36.0	36.0	36.0	36.0
24	34.94330056286125	36.0	36.0	36.0	36.0	36.0
25	34.92084921376107	36.0	36.0	36.0	36.0	36.0
26	34.8724917337641	36.0	36.0	36.0	32.0	36.0
27	34.84799906610465	36.0	36.0	36.0	32.0	36.0
28	34.83919647138999	36.0	36.0	36.0	32.0	36.0
29	34.81029935132133	36.0	36.0	36.0	32.0	36.0
30	34.793315707110224	36.0	36.0	36.0	32.0	36.0
31	34.792937100885936	36.0	36.0	36.0	32.0	36.0
32	34.760783967288425	36.0	36.0	36.0	32.0	36.0
33	34.74647580706227	36.0	36.0	36.0	32.0	36.0
34	34.71620939448245	36.0	36.0	36.0	32.0	36.0
35	34.7132373356218	36.0	36.0	36.0	32.0	36.0
36	34.68484817890406	36.0	36.0	36.0	32.0	36.0
37	34.6672272142154	36.0	36.0	36.0	32.0	36.0
38	34.63835217950983	36.0	36.0	36.0	32.0	36.0
39	34.64036825765415	36.0	36.0	36.0	32.0	36.0
40	34.612483909235465	36.0	36.0	36.0	32.0	36.0
41	34.6134083394331	36.0	36.0	36.0	32.0	36.0
42	34.564571291552035	36.0	36.0	36.0	32.0	36.0
43	34.56493412251697	36.0	36.0	36.0	32.0	36.0
44	34.50606085464045	36.0	36.0	36.0	32.0	36.0
45	34.491427724071784	36.0	36.0	36.0	32.0	36.0
46	34.46032837779853	36.0	36.0	36.0	32.0	36.0
47	34.44911216840405	36.0	36.0	36.0	32.0	36.0
48	34.423120851106795	36.0	36.0	36.0	32.0	36.0
49	34.38570824604356	36.0	36.0	36.0	32.0	36.0
50	33.81456498144829	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	0.0
20	1.0
21	16.0
22	39.0
23	111.0
24	260.0
25	585.0
26	1187.0
27	2189.0
28	3539.0
29	5391.0
30	7577.0
31	10691.0
32	14910.0
33	23449.0
34	48653.0
35	198353.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.574151205351505	17.888110564180234	12.899154360721948	26.638583869746306
2	15.738710400030289	20.80605519465154	36.870052468678125	26.58518193664005
3	18.395646000946524	23.795551348793182	28.738917810380187	29.069884839880107
4	12.846109190035085	17.537355814129583	34.640260985890606	34.976274009944724
5	14.65300739544158	36.79642343320124	33.2757010525253	15.274868118831876
6	33.800386178348774	34.92579318003988	17.552500063101036	13.72132057851031
7	30.437731896312375	30.657954516772257	20.243443802216106	18.660869784699262
8	28.484123778994924	32.82200459375552	19.22877912112875	19.4650925061208
9	28.12007016923912	14.953241541199185	17.903883286848316	39.022805002713376
10	15.77114082525099	27.642563717478687	32.65982217791043	23.926473279359893
11	36.44873671723163	21.526603397359853	22.62613897372473	19.39852091168379
12	25.29831203659883	23.693327023189777	28.510175106483672	22.498185833727717
13	29.998643323415923	20.68900240100205	24.57666957984042	24.735684695741615
14	24.252329224391154	19.80937116462797	25.377424270628584	30.560875340352293
15	25.01482949238963	26.97642426231163	22.671454173713304	25.33729207158543
16	25.736317199297048	26.21052598367561	23.576663753474534	24.476493063552812
17	23.918448219288724	26.096065019308917	25.02807996163457	24.95740679976779
18	24.275437766209183	26.18141662722827	25.639375295787982	23.90377031077457
19	25.863537696559728	25.44517781872334	25.24041495242182	23.45086953229511
20	25.96277015302098	24.79034548035968	24.916863858652786	24.33002050796656
21	26.645675054897904	24.60214795931245	24.642532623236328	24.109644362553322
22	26.26202074762109	24.963716903505894	24.313460713294127	24.46080163557889
23	25.402194043836708	24.94281414360039	24.9790975835229	24.675894229040004
24	25.28671805243115	25.38452558613531	24.63866426459777	24.69009209683577
25	24.958983808069462	24.929325946211996	24.90850234107803	25.20318790464051
26	24.954959787214577	25.297927992452806	25.120606804463954	24.626505415868667
27	25.107039524955905	25.369550607530154	24.74040746011062	24.783002407403316
28	25.59158715735272	24.63779090312484	24.762734110757737	25.0078878287647
29	24.99313765392952	25.494798814943824	24.615787497594237	24.89627603353242
30	25.082191694379414	25.309678111452566	24.40635826113295	25.201771933035065
31	24.854460834582316	25.049144119271123	25.184507375562042	24.911887670584523
32	25.183864708387894	25.09962296297465	24.87371625991891	24.84279606871855
33	24.816207790896527	25.126998050067208	24.947465402891453	25.109328756144812
34	25.144818708431664	24.906293776897158	25.135668942539468	24.813218572131706
35	25.19861427498691	25.04937749648205	25.124153641314294	24.62785458721675
36	24.894927490502454	24.80342290265174	25.279877825598568	25.02177178124724
37	25.083531843064254	25.05229613970878	24.907160548360125	24.95701146886684
38	25.17851226630906	24.945018537508876	24.908416817859113	24.968052378322948
39	25.07485573115671	24.92940370981533	24.794994683586637	25.200745875441328
40	25.388236481924366	24.794759990660868	25.106169505215398	24.710834022199364
41	25.542998132162147	24.208693018325004	25.367257307284568	24.88105154222828
42	24.86085870059064	24.65135796860013	25.449921752738653	25.037861578070576
43	24.88294462113181	24.220051491746176	25.682454944722092	25.214548942399915
44	24.491160982751058	24.446043029276186	25.602387780922363	25.46040820705039
45	24.54945290710148	24.757373449272436	25.36630614485657	25.326867498769513
46	24.841220511817358	24.300755011058563	25.118867704267878	25.7391567728562
47	24.46481250690183	24.640552777295746	26.045843916136867	24.848790799665558
48	24.57556262994993	24.561049253980922	25.644188812711192	25.219199303357954
49	24.35518423983518	24.685834718931318	25.705235194083624	25.253745847149872
50	24.01654550961684	25.113269221061135	25.5653995658539	25.30478570346812
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	2.5
2	3.0
3	12.0
4	21.0
5	21.5
6	22.0
7	17.0
8	12.0
9	22.0
10	32.0
11	56.0
12	80.0
13	146.0
14	212.0
15	273.0
16	334.0
17	331.0
18	328.0
19	314.5
20	301.0
21	386.0
22	471.0
23	659.5
24	848.0
25	1029.5
26	1211.0
27	1628.5
28	2046.0
29	2580.0
30	3114.0
31	3881.0
32	4648.0
33	5768.0
34	6888.0
35	8150.0
36	9412.0
37	10756.5
38	12101.0
39	13038.0
40	13975.0
41	15112.0
42	16249.0
43	17363.5
44	18478.0
45	20252.0
46	22026.0
47	23176.0
48	24326.0
49	24858.0
50	25390.0
51	26431.5
52	27473.0
53	27521.5
54	27570.0
55	26422.0
56	25274.0
57	23367.0
58	21460.0
59	19812.5
60	18165.0
61	14950.5
62	11736.0
63	9893.0
64	8050.0
65	6841.5
66	5633.0
67	4798.0
68	3963.0
69	3068.0
70	2173.0
71	1658.0
72	1143.0
73	959.0
74	775.0
75	599.5
76	424.0
77	346.0
78	268.0
79	220.0
80	172.0
81	126.0
82	80.0
83	55.0
84	30.0
85	22.5
86	15.0
87	9.0
88	3.0
89	3.0
90	3.0
91	2.5
92	2.0
93	2.0
94	2.0
95	3.0
96	4.0
97	4.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010096165980968727
2	3.155051869052727E-4
3	6.310103738105454E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0012620207476210909
10	0.0018930311214316361
11	0.0
12	6.310103738105454E-4
13	3.155051869052727E-4
14	3.155051869052727E-4
15	0.0050480829904843635
16	9.465155607158181E-4
17	0.0
18	6.310103738105454E-4
19	0.0
20	6.310103738105454E-4
21	0.0
22	0.0
23	9.465155607158181E-4
24	9.465155607158181E-4
25	0.0012620207476210909
26	0.004101567429768546
27	0.004101567429768546
28	0.0025240414952421818
29	0.0015775259345263636
30	0.003155051869052727
31	0.008518640046442363
32	0.002208536308336909
33	0.005679093364294909
34	0.0012620207476210909
35	0.0018930311214316361
36	0.008834145233347636
37	0.002208536308336909
38	0.008518640046442363
39	0.0028395466821474545
40	0.0018930311214316361
41	0.0025240414952421818
42	0.0025240414952421818
43	0.0025240414952421818
44	0.0015775259345263636
45	0.0012620207476210909
46	9.465155607158181E-4
47	0.002208536308336909
48	9.465155607158181E-4
49	3.155051869052727E-4
50	0.0025240414952421818
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	316952.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.11332544845644
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.91819927672765	26.263861511514836
2	15.541958571304837	13.401310359821853
3	7.808262448808881	10.099204804273763
4	4.678830158783724	8.068797094147834
5	2.983165613873715	6.430709498879092
6	1.9953321410851155	5.16152423858208
7	1.420431306151413	4.286766202549737
8	0.9848229756452248	3.39671947664879
9	0.7541531365982007	2.926264465451921
>10	2.8671693205150146	18.460583831367046
>50	0.04180735198243143	1.1810816817553356
>100	0.005867698523850025	0.3231768350077518
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	3.155051869052727E-4	0.0
22	0.0	0.0	0.0	3.155051869052727E-4	0.0
23	0.0	0.0	0.0	3.155051869052727E-4	0.0
24	0.0	0.0	0.0	6.310103738105454E-4	0.0
25	0.0	0.0	0.0	0.0015775259345263636	0.0
26	0.0	0.0	0.0	0.0015775259345263636	0.0
27	0.0	0.0	0.0	0.0018930311214316363	0.0
28	0.0	0.0	0.0	0.005679093364294909	0.0
29	0.0	0.0	0.0	0.011358186728589818	0.0
30	0.0	0.0	0.0	0.022716373457179636	0.0
31	0.0	0.0	0.0	0.05931497513819127	0.0
32	0.0	0.0	0.0	0.10443221686564527	0.0
33	0.0	0.0	0.0	0.15522855195739418	0.0
34	0.0	0.0	0.0	0.2035008455539009	0.0
35	0.0	0.0	0.0	0.2587142532623236	0.0
36	0.0	0.0	0.0	0.33727504480173653	0.0
37	0.0	0.0	0.0	0.4694717181150458	0.0
38	0.0	0.0	0.0	0.6707640273606098	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	395	0.0	33.415207	1
AAGAGCG	35	3.210995E-4	31.431162	9
GGTATCA	110	0.0	29.997744	1
TAAGGGT	45	4.0622755E-5	29.331123	4
CTTTACG	30	0.005740815	29.331123	2
TACACCG	30	0.005740815	29.331123	5
ATTAGAG	50	0.002577405	21.998344	3
TTTGGAC	80	3.6039783E-6	21.998344	3
TATCAAC	590	0.0	21.998343	2
TTAGGGT	60	2.8677675E-4	21.998343	4
ATCAACG	630	0.0	20.601624	3
TCAACGC	635	0.0	20.439405	4
CAACGCA	655	0.0	20.151155	5
CCTTTAC	55	0.0044783927	19.998495	1
TATTAGA	55	0.0044783927	19.998495	2
AACGCAG	685	0.0	19.268621	6
CCTTAGG	80	8.967123E-5	19.24855	2
GCGGTGC	60	0.0073968256	18.334845	13
GAGCGGT	60	0.0073968256	18.334845	11
GTAATAT	85	1.4274423E-4	18.116282	3
>>END_MODULE