FastQCFastQC Report
Thu 2 Feb 2017
SRR4063866_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063866_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5746460
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT78620.13681466502855671No Hit
GTATCAACGCAGAGTACTTTTTTTT76890.13380411592528269No Hit
CTGTAGGACGTGGAATATGGCAAGA75030.13056734058881467No Hit
GTCCTACAGTGGACATTTCTAAATT73470.12785262579048667No Hit
CTTTAGGACGTGAAATATGGCGAGG66460.1156538112159486No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG60730.10568245493747454No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA76650.013.6425971
GGTATCA23550.012.8250221
CCGATTG550.003067955112.0920789
CGGTCCA10100.011.94638710
CGAACGA4250.011.62526116
TCGCGTA5750.011.5663379
GTCGCGT5750.011.5662368
CGTCGTA9400.011.5220910
AACCGCG3850.011.3507597
GCGTAAC5900.011.271687511
AGGACCT29150.011.0793745
CCGTCGT9700.011.068089
ACGGTAT8950.011.04017459
TCCAACG7300.010.93284918
CGCGTAA6100.010.90240910
GTATTAG7950.010.8716671
GCGTTAT4900.010.85461
TAGAAAT13550.010.7252664
GACCGTA803.7736745E-410.6876037
TGTAGGA48050.010.6516082
Produced by FastQC (version 0.11.5)