##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063866_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5746460
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.262189069444492	32.0	32.0	32.0	32.0	32.0
2	30.82965060228384	32.0	32.0	32.0	32.0	32.0
3	30.855952186215514	32.0	32.0	32.0	32.0	32.0
4	30.892333540997413	32.0	32.0	32.0	32.0	32.0
5	30.78015595688476	32.0	32.0	32.0	32.0	32.0
6	34.45139094329378	36.0	36.0	36.0	32.0	36.0
7	34.39542761978679	36.0	36.0	36.0	32.0	36.0
8	34.352501888118944	36.0	36.0	36.0	32.0	36.0
9	34.504009946993456	36.0	36.0	36.0	32.0	36.0
10	34.169853788245284	36.0	36.0	36.0	32.0	36.0
11	34.4694215221197	36.0	36.0	36.0	32.0	36.0
12	34.271515854978546	36.0	36.0	36.0	32.0	36.0
13	34.367822450691385	36.0	36.0	36.0	32.0	36.0
14	34.259144934446596	36.0	36.0	36.0	32.0	36.0
15	34.18870435015644	36.0	36.0	36.0	32.0	36.0
16	34.19341855681585	36.0	36.0	36.0	32.0	36.0
17	34.12046947163993	36.0	36.0	36.0	32.0	36.0
18	34.11492031615986	36.0	36.0	36.0	32.0	36.0
19	34.12512468545852	36.0	36.0	36.0	32.0	36.0
20	34.0942072162688	36.0	36.0	36.0	32.0	36.0
21	34.07743828374338	36.0	36.0	36.0	32.0	36.0
22	34.05099034884085	36.0	36.0	36.0	32.0	36.0
23	33.99230848209158	36.0	36.0	36.0	32.0	36.0
24	33.97243763986872	36.0	36.0	36.0	32.0	36.0
25	33.57047625842693	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	12.0
4	59.0
5	68.0
6	209.0
7	96.0
8	344.0
9	372.0
10	490.0
11	173.0
12	368.0
13	307.0
14	419.0
15	791.0
16	1447.0
17	1932.0
18	2609.0
19	3620.0
20	5596.0
21	8533.0
22	13253.0
23	20620.0
24	30995.0
25	45549.0
26	64480.0
27	85683.0
28	116911.0
29	157213.0
30	207717.0
31	283040.0
32	398470.0
33	561146.0
34	1194368.0
35	2539570.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.647858758768606	17.43038866059157	11.949674585648344	25.972077994991484
2	16.218272592225453	19.442717680194132	38.93816644785296	25.400843279727454
3	18.951511998009178	23.700283831357883	28.40354867507661	28.944655495556326
4	12.327039493903333	15.994193782592973	36.290842695084486	35.38792402841921
5	14.425161500624876	36.790642039710384	33.80666357695581	14.977532882708925
6	33.83411223248765	35.91317426757605	17.126238052837977	13.126475447098326
7	30.311534949555853	30.262502423776787	20.650038658587064	18.775923968080296
8	27.6103062777006	34.04967568753199	19.080470575128036	19.259547459639375
9	27.410488679777124	14.46863401883712	18.531340387721485	39.58953691366427
10	16.000282074922232	26.73281497713365	31.47552172978093	25.79138121816319
11	37.086746857050926	20.782900936250943	23.213336787774068	18.917015418924066
12	25.514025575511702	23.114412427893978	29.186842073644392	22.184719922949927
13	29.599176977376978	19.99187146749266	25.18380618012257	25.22514537500779
14	23.339726546473795	20.378985077704183	24.99524601274556	31.28604236307646
15	24.80282192402898	28.897819131079196	21.841302642924504	24.458056301967325
16	25.270410920117182	25.772008701907428	24.86161003397561	24.09597034399978
17	23.56331476243088	26.332216293115536	25.598887661276493	24.505581283177097
18	24.23648291984013	25.154784567711708	27.258837623669606	23.349894888778554
19	25.79018879963888	24.48765031768467	25.15661321186478	24.56554767081167
20	26.0147616899662	24.683910698393735	25.00721887740796	24.294108734232108
21	26.717938920861968	24.21687529616484	24.09646758178155	24.968718201191642
22	25.99621630159939	25.1805250469305	24.73714302731748	24.086115624152626
23	24.03177366566488	25.061723492685832	25.55761881075689	25.3488840308924
24	24.970598392590222	24.97819152206196	25.525663123148195	24.525546962199627
25	24.628548609257084	25.003679029774105	25.643299036938856	24.72447332402996
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	198.0
1	198.0
2	594.0
3	990.0
4	990.0
5	990.0
6	2433.0
7	3876.0
8	3876.0
9	3876.0
10	4689.5
11	5503.0
12	5503.0
13	5503.0
14	8103.0
15	10703.0
16	10703.0
17	10703.0
18	20041.0
19	29379.0
20	29379.0
21	29379.0
22	54261.0
23	79143.0
24	79143.0
25	79143.0
26	127766.5
27	176390.0
28	176390.0
29	176390.0
30	237996.0
31	299602.0
32	299602.0
33	299602.0
34	355026.5
35	410451.0
36	410451.0
37	410451.0
38	458059.0
39	505667.0
40	505667.0
41	505667.0
42	569473.5
43	633280.0
44	633280.0
45	633280.0
46	687688.5
47	742097.0
48	742097.0
49	742097.0
50	750257.5
51	758418.0
52	758418.0
53	758418.0
54	693426.0
55	628434.0
56	628434.0
57	628434.0
58	572866.5
59	517299.0
60	517299.0
61	517299.0
62	455618.5
63	393938.0
64	393938.0
65	393938.0
66	327515.0
67	261092.0
68	261092.0
69	261092.0
70	198259.5
71	135427.0
72	135427.0
73	135427.0
74	104979.0
75	74531.0
76	74531.0
77	74531.0
78	60054.5
79	45578.0
80	45578.0
81	45578.0
82	31469.5
83	17361.0
84	17361.0
85	17361.0
86	13073.5
87	8786.0
88	8786.0
89	8786.0
90	6274.5
91	3763.0
92	3763.0
93	3763.0
94	2677.5
95	1592.0
96	1592.0
97	1592.0
98	2277.0
99	2962.0
100	2962.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.039067530270810204
2	0.005638253811912029
3	0.0015661816144200082
4	0.0035848156952280187
5	0.008822823094566046
6	0.012947101345872066
7	0.022518211211772117
8	0.034804035876000186
9	0.04874305224433825
10	0.0575136692850903
11	0.05443351211006428
12	0.06440486838853833
13	0.06483991883698834
14	0.06807669417345635
15	0.05965411749146431
16	0.06631909036171835
17	0.06273427466649033
18	0.0773867737702864
19	0.0746198529181444
20	0.07952722197666041
21	0.07488088318721439
22	0.0775955979855424
23	0.08220713273911243
24	0.0770387334115264
25	0.0775607939496664
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	5746460.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.28639770077436
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.35524893973105	19.508711170592143
2	15.8853731367836	9.939922031652184
3	7.4396361886440525	6.982782496409731
4	4.148473772443045	5.1916320118353925
5	2.484194034154868	3.8860741259230123
6	1.6789772276677963	3.1517489565214953
7	1.1419789243125753	2.5009884754362015
8	0.790158582826325	1.9776972535187731
9	0.6244754659563628	1.7583829004058433
>10	3.057107250369504	17.037633878177335
>50	0.21520351138684773	4.65784474575193
>100	0.1440339636024772	8.744062688222298
>500	0.01893819535505092	4.099909522595229
>1k	0.01536283400263777	8.941323761421293
>5k	8.379727637796174E-4	1.621285981537051
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	7862	0.13681466502855671	No Hit
GTATCAACGCAGAGTACTTTTTTTT	7689	0.13380411592528269	No Hit
CTGTAGGACGTGGAATATGGCAAGA	7503	0.13056734058881467	No Hit
GTCCTACAGTGGACATTTCTAAATT	7347	0.12785262579048667	No Hit
CTTTAGGACGTGAAATATGGCGAGG	6646	0.1156538112159486	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	6073	0.10568245493747454	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	1.740201793800009E-5	0.0
12	0.0	0.0	0.0	1.740201793800009E-5	0.0
13	0.0	0.0	0.0	1.740201793800009E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7665	0.0	13.642597	1
GGTATCA	2355	0.0	12.825022	1
CCGATTG	55	0.0030679551	12.092078	9
CGGTCCA	1010	0.0	11.946387	10
CGAACGA	425	0.0	11.625261	16
TCGCGTA	575	0.0	11.566337	9
GTCGCGT	575	0.0	11.566236	8
CGTCGTA	940	0.0	11.52209	10
AACCGCG	385	0.0	11.350759	7
GCGTAAC	590	0.0	11.2716875	11
AGGACCT	2915	0.0	11.079374	5
CCGTCGT	970	0.0	11.06808	9
ACGGTAT	895	0.0	11.0401745	9
TCCAACG	730	0.0	10.932849	18
CGCGTAA	610	0.0	10.902409	10
GTATTAG	795	0.0	10.871667	1
GCGTTAT	490	0.0	10.8546	1
TAGAAAT	1355	0.0	10.725266	4
GACCGTA	80	3.7736745E-4	10.687603	7
TGTAGGA	4805	0.0	10.651608	2
>>END_MODULE