FastQCFastQC Report
Thu 2 Feb 2017
SRR4063866_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063866_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5746460
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA81550.027.6775421
GGTATCA32950.021.9656771
TGTAGGA59300.019.957462
GATATAC23350.019.5023781
GTAGGAC58400.019.323343
TTAGGAC34700.019.0815983
TAGGACG55350.018.9577144
TCAACGC120050.018.7638974
AACGCAG121950.018.4354766
ATCAACG122200.018.397443
CTGTAGG61300.018.374421
GACGTGA30600.018.1879757
CAACGCA123600.018.1715725
AGGACGT57100.017.9914445
TATCAAC125500.017.9837932
GTCCTAC47750.017.967821
GGACGTG56400.017.9027126
ATACCGT17550.016.9216186
CGTCGTA18400.016.85797510
TCCTACA52600.016.8537672
Produced by FastQC (version 0.11.5)