##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063866_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5746460
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.3514253992893	32.0	32.0	32.0	32.0	32.0
2	31.40482958203833	32.0	32.0	32.0	32.0	32.0
3	31.48659522558236	32.0	32.0	32.0	32.0	32.0
4	31.56889458901654	32.0	32.0	32.0	32.0	32.0
5	31.51519648618454	32.0	32.0	32.0	32.0	32.0
6	35.117063200648744	36.0	36.0	36.0	36.0	36.0
7	35.13263713660236	36.0	36.0	36.0	36.0	36.0
8	35.074951187339686	36.0	36.0	36.0	36.0	36.0
9	35.19284115786066	36.0	36.0	36.0	36.0	36.0
10	35.0406939228673	36.0	36.0	36.0	36.0	36.0
11	35.18765361631335	36.0	36.0	36.0	36.0	36.0
12	35.09037198553544	36.0	36.0	36.0	36.0	36.0
13	35.13810450259812	36.0	36.0	36.0	36.0	36.0
14	35.077768748064024	36.0	36.0	36.0	36.0	36.0
15	35.04721619919046	36.0	36.0	36.0	36.0	36.0
16	35.06451537120245	36.0	36.0	36.0	36.0	36.0
17	35.02927158633315	36.0	36.0	36.0	36.0	36.0
18	35.031206690727856	36.0	36.0	36.0	36.0	36.0
19	35.024550592886754	36.0	36.0	36.0	36.0	36.0
20	35.01392683495578	36.0	36.0	36.0	36.0	36.0
21	35.007476254946525	36.0	36.0	36.0	36.0	36.0
22	34.987032016232604	36.0	36.0	36.0	36.0	36.0
23	34.93545695958903	36.0	36.0	36.0	36.0	36.0
24	34.90816868124028	36.0	36.0	36.0	32.0	36.0
25	34.8790796420753	36.0	36.0	36.0	32.0	36.0
26	34.8160551017496	36.0	36.0	36.0	32.0	36.0
27	34.80705390797117	36.0	36.0	36.0	32.0	36.0
28	34.781061383878075	36.0	36.0	36.0	32.0	36.0
29	34.74695412480031	36.0	36.0	36.0	32.0	36.0
30	34.7248523438778	36.0	36.0	36.0	32.0	36.0
31	34.72269153531043	36.0	36.0	36.0	32.0	36.0
32	34.68368682632438	36.0	36.0	36.0	32.0	36.0
33	34.67480379224775	36.0	36.0	36.0	32.0	36.0
34	34.66629542361732	36.0	36.0	36.0	32.0	36.0
35	34.629876480476675	36.0	36.0	36.0	32.0	36.0
36	34.600514403650244	36.0	36.0	36.0	32.0	36.0
37	34.57781503743174	36.0	36.0	36.0	32.0	36.0
38	34.533368717436474	36.0	36.0	36.0	32.0	36.0
39	34.51128538265297	36.0	36.0	36.0	32.0	36.0
40	34.50834461564163	36.0	36.0	36.0	32.0	36.0
41	34.49853683833177	36.0	36.0	36.0	32.0	36.0
42	34.44347981191899	36.0	36.0	36.0	32.0	36.0
43	34.4418770860669	36.0	36.0	36.0	32.0	36.0
44	34.366485801693564	36.0	36.0	36.0	32.0	36.0
45	34.346029207546906	36.0	36.0	36.0	32.0	36.0
46	34.30161368912339	36.0	36.0	36.0	32.0	36.0
47	34.29476529898407	36.0	36.0	36.0	32.0	36.0
48	34.248893405679325	36.0	36.0	36.0	32.0	36.0
49	34.22768591445864	36.0	36.0	36.0	32.0	36.0
50	33.64853892657393	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	2.0
17	6.0
18	2.0
19	16.0
20	52.0
21	172.0
22	566.0
23	1598.0
24	4383.0
25	9845.0
26	20566.0
27	37714.0
28	62730.0
29	97680.0
30	142164.0
31	203928.0
32	297048.0
33	483537.0
34	1015350.0
35	3369100.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.7194685101413	18.278456815143716	12.508229693866701	26.493844980848287
2	15.487750846698425	20.188923990827014	38.380993582459254	25.942331580015303
3	18.252976887595413	24.279144750191335	28.707522816743168	28.76035554547008
4	12.001371279013515	16.629733783929584	36.3276347525259	35.041260184531
5	14.058794457805327	37.277193263330815	33.51009143020225	15.153920848661611
6	33.97925642130893	36.158235349701805	16.906463865622847	12.956044363366411
7	30.296645342226462	30.34392664141981	20.64268110895442	18.716746907399305
8	27.658406681401537	34.164413249968376	18.934895385140656	19.242284683489434
9	27.431956011362256	14.271526503669346	18.455994917159337	39.84052256780906
10	15.969202342122763	26.95708677990488	31.429600145203672	25.644110732768677
11	37.214023938215874	20.72961440608653	23.145414742293514	18.91094691340408
12	25.46243743749405	23.19956327842584	29.11590854393136	22.22209074014875
13	29.704581801222673	19.901190826304333	25.224617719175207	25.169609653297787
14	23.40566673731903	20.316905440336512	24.965604827749186	31.311822994595268
15	24.83682287977641	28.955798691659375	21.75465219683931	24.45272623172491
16	25.37026167124547	25.827260120189845	24.724588869326915	24.077889339237775
17	23.642190148369604	26.460864601859228	25.429220772440775	24.467724477330393
18	24.353657138054174	25.146773301833292	27.141797357567203	23.35777220254533
19	25.899771337484296	24.518120721278837	25.044949412333857	24.53715852890301
20	26.173273356312265	24.66758886354172	24.93993219782115	24.219205582324864
21	26.84565802250429	24.191432638528767	24.078580552200833	24.884328786766112
22	26.073258085729783	25.109933069993986	24.78030352995266	24.036505314323573
23	24.10105770201273	25.03301185781756	25.520846022393073	25.345084417776633
24	25.08047163154199	24.99255625444297	25.4203519225398	24.506620191475236
25	24.819607417291934	24.947305736017746	25.63137403947824	24.601712807212085
26	23.960534987495112	26.016258181778312	25.96518053034787	24.058026300378703
27	24.78725294514947	25.35312817658732	25.338788122745985	24.520830755517224
28	24.126999154055966	25.485069113854042	25.482702350039844	24.905229382050152
29	24.39117090129208	24.808742712941175	25.365969193833198	25.43411719193355
30	24.027883233044722	25.21465742260734	25.944559659701483	24.812899684646453
31	25.359457127412927	24.65027133535421	24.919187626369947	25.071083910862917
32	24.698995834860412	25.45367722273908	24.764882099088346	25.082444843312164
33	24.077547205306296	25.18616992117787	25.720015683329017	25.016267190186813
34	24.811112843630532	25.330195771600728	25.86464514135038	23.99404624341836
35	25.083932269460345	25.187337939195658	25.718497119885843	24.010232671458155
36	23.971616591829527	25.49158697835533	25.640959597691136	24.895836832124008
37	25.035971248061344	25.320591355484336	24.756380502365367	24.887056894088953
38	24.611333903817247	25.393895822119006	25.08914463786019	24.905625636203556
39	25.576762971336926	24.623525636384898	24.91122841359032	24.888482978687858
40	26.082598340431794	24.8238087929062	25.264839338748285	23.828753527913722
41	24.74337194283441	24.966839266067947	26.225676762635274	24.064112028462368
42	25.64175921667186	25.725429676875056	25.35451616007579	23.278294946377294
43	24.525492570975118	24.945356902954554	25.831010131595892	24.698140394474436
44	24.060003699721165	25.495881841417006	25.205925331413052	25.23818912744878
45	24.36976778747781	25.724482952206035	25.182680417250946	24.72306884306521
46	24.39630836312303	24.825829903066165	25.287823981439487	25.490037752371315
47	25.122652154452613	24.83593012029239	25.47010858404979	24.57130914120521
48	25.076465957160966	25.401367964488053	24.62283871425817	24.89932736409281
49	24.828036980815423	25.275688126159523	25.048121105957964	24.848153787067087
50	24.66836895842611	26.104858179188223	24.47845779536337	24.7483150670223
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	22.0
2	36.0
3	119.5
4	203.0
5	279.5
6	356.0
7	343.5
8	331.0
9	430.5
10	530.0
11	874.0
12	1218.0
13	2351.5
14	3485.0
15	4842.0
16	6199.0
17	6719.5
18	7240.0
19	7719.0
20	8198.0
21	9700.5
22	11203.0
23	13487.0
24	15771.0
25	21684.0
26	27597.0
27	40729.0
28	53861.0
29	68254.5
30	82648.0
31	98913.0
32	115178.0
33	140384.0
34	165590.0
35	198522.5
36	231455.0
37	251458.5
38	271462.0
39	279967.5
40	288473.0
41	299158.5
42	309844.0
43	310684.0
44	311524.0
45	338703.0
46	365882.0
47	386194.0
48	406506.0
49	419734.5
50	432963.0
51	416617.0
52	400271.0
53	394545.5
54	388820.0
55	386645.5
56	384471.0
57	372804.0
58	361137.0
59	330384.0
60	299631.0
61	263395.0
62	227159.0
63	197621.5
64	168084.0
65	144977.5
66	121871.0
67	106908.5
68	91946.0
69	82963.0
70	73980.0
71	56506.5
72	39033.0
73	33565.0
74	28097.0
75	21819.0
76	15541.0
77	13378.5
78	11216.0
79	9403.0
80	7590.0
81	6057.0
82	4524.0
83	3597.5
84	2671.0
85	1931.5
86	1192.0
87	882.5
88	573.0
89	432.5
90	292.0
91	251.0
92	210.0
93	171.0
94	132.0
95	124.0
96	116.0
97	93.0
98	70.0
99	71.0
100	72.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008683606951062045
2	3.82844394636002E-4
3	3.82844394636002E-4
4	0.0
5	0.0
6	1.2181412556600063E-4
7	3.480403587600018E-5
8	1.740201793800009E-5
9	0.0011659352018460062
10	8.526988789620045E-4
11	0.0
12	1.2181412556600063E-4
13	5.220605381400027E-4
14	2.4362825113200125E-4
15	0.004350504484500023
16	8.875029148380047E-4
17	0.0
18	2.262262331940012E-4
19	0.0
20	6.438746637060034E-4
21	0.0
22	1.5661816144200082E-4
23	6.960807175200036E-4
24	4.3505044845000226E-4
25	0.0018098098655520095
26	0.0053424195069660275
27	0.005516439686346029
28	0.0037762378925460197
29	0.002488488565134013
30	0.0036718257849180187
31	0.0075524757850920395
32	0.0033759914799720175
33	0.004019866143678021
34	0.0029931470853360154
35	0.002784322870080014
36	0.007430661659526038
37	0.0035500116593520188
38	0.008022330269418041
39	0.004385308520376023
40	0.0025406946189480135
41	0.0035848156952280187
42	0.0018620159193660096
43	0.001392161435040007
44	0.0014095634529780073
45	0.0014965735426680077
46	0.001148533183908006
47	0.0022274582960640117
48	0.00194902600905601
49	3.654423766980019E-4
50	0.001548779596482008
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	5746460.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.53118583765564
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.68648126129555	21.991697197801706
2	15.625073622391303	10.791046419636906
3	7.083370591253396	7.3379155873066715
4	4.0076073993793	5.535497434893219
5	2.432522417336126	4.199894182364855
6	1.5536018100001279	3.218862769129955
7	1.0798287779111582	2.610143774202915
8	0.7919722794321132	2.187819356747363
9	0.5914833969392349	1.8382160789636903
>10	2.8222942545568412	17.098234498099426
>50	0.1849902308994307	4.383223823192063
>100	0.1144894932298815	7.487070576985433
>500	0.014816734362891725	3.532273299899598
>1k	0.011061793631549018	7.022241413344682
>5k	4.0593738097222706E-4	0.7658635874317082
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	1.740201793800009E-5	0.0
14	0.0	0.0	0.0	1.740201793800009E-5	0.0
15	0.0	0.0	0.0	5.2206053814000275E-5	0.0
16	0.0	0.0	0.0	6.960807175200037E-5	0.0
17	0.0	0.0	0.0	1.0441210762800055E-4	0.0
18	0.0	0.0	0.0	1.2181412556600063E-4	0.0
19	0.0	0.0	0.0	1.5661816144200082E-4	0.0
20	0.0	0.0	0.0	2.088242152560011E-4	0.0
21	0.0	0.0	0.0	3.306383408220017E-4	0.0
22	0.0	0.0	0.0	4.3505044845000226E-4	0.0
23	0.0	0.0	0.0	5.916686098920031E-4	0.0
24	0.0	0.0	0.0	0.0010093170404040053	0.0
25	0.0	0.0	0.0	0.0013747594171020072	0.0
26	0.0	0.0	0.0	0.0015835836323580081	0.0
27	0.0	0.0	0.0	0.0020186340808080105	0.0
28	0.0	0.0	0.0	0.002853930941832015	0.0
29	0.0	0.0	0.0	0.004159082287182022	0.0
30	0.0	0.0	0.0	0.007187033408394037	0.0
31	0.0	0.0	0.0	0.018219912781086096	0.0
32	0.0	0.0	0.0	0.031532456503656166	0.0
33	0.0	0.0	0.0	0.043244014575930224	0.0
34	0.0	0.0	0.0	0.058035729823230305	0.0
35	0.0	0.0	0.0	0.0758205921558664	0.0
36	0.0	0.0	0.0	0.10058366368164053	0.0
37	0.0	1.740201793800009E-5	0.0	0.14168723005119674	0.0
38	0.0	1.740201793800009E-5	0.0	0.19580750583837703	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	8155	0.0	27.677542	1
GGTATCA	3295	0.0	21.965677	1
TGTAGGA	5930	0.0	19.95746	2
GATATAC	2335	0.0	19.502378	1
GTAGGAC	5840	0.0	19.32334	3
TTAGGAC	3470	0.0	19.081598	3
TAGGACG	5535	0.0	18.957714	4
TCAACGC	12005	0.0	18.763897	4
AACGCAG	12195	0.0	18.435476	6
ATCAACG	12220	0.0	18.39744	3
CTGTAGG	6130	0.0	18.37442	1
GACGTGA	3060	0.0	18.187975	7
CAACGCA	12360	0.0	18.171572	5
AGGACGT	5710	0.0	17.991444	5
TATCAAC	12550	0.0	17.983793	2
GTCCTAC	4775	0.0	17.96782	1
GGACGTG	5640	0.0	17.902712	6
ATACCGT	1755	0.0	16.921618	6
CGTCGTA	1840	0.0	16.857975	10
TCCTACA	5260	0.0	16.853767	2
>>END_MODULE