FastQCFastQC Report
Thu 2 Feb 2017
SRR4063865_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063865_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3609716
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46660.1292622466698211No Hit
GAATAGGACCGCGGTTCTATTTTGT45740.12671356971019326No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG44750.12397097167755025No Hit
CTATTGGAGCTGGAATTACCGCGGC44460.12316758437505887No Hit
GTATCTGATCGTCTTCGAACCTCCG43490.12048039236327733No Hit
GGGTAGGCACACGCTGAGCCAGTCA41950.11621412875694377No Hit
GCGCAAGACGGACCAGAGCGAAAGC40690.11272354944267084No Hit
GTCTTGCGCCGGTCCAAGAATTTCA39490.10939918819098235No Hit
GTCCTATTCCATTATTCCTAGCTGC38800.10748768047126145No Hit
GTACATGGGGAATAATTGCAATCCC37640.1042741312612959No Hit
GATTAAGAGGGACGGCCGGGGGCAT37310.10335993191708157No Hit
GAACTACGACGGTATCTGATCGTCT37090.10275046568760533No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAC250.006042646718.9950681
GTATCAA46100.012.9998771
CCGTCGT7100.012.8460079
CGTCGTA7500.012.79355710
GGTATCA14550.012.6633771
GCGTTAT3500.012.2111141
ATTTGCG5900.011.75574616
CGAACGA2450.011.63414816
CGCCAGT5150.011.62365718
ACCGTCG7850.011.6188438
CGTTATT3600.011.6058442
TTTGCGC5950.011.49759417
CGGCTAA752.0738585E-411.4008319
CGCATCG5100.011.36373213
GTCCTAG3700.011.2943641
CGGACAT4050.011.257745
TCGCGTA3050.011.2139349
GTCGTAG8000.011.16315911
TTGCGCG6150.011.12415118
CGCGCTA1303.2647222E-810.96294616
Produced by FastQC (version 0.11.5)