##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063865_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3609716
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22254880993408	32.0	32.0	32.0	32.0	32.0
2	30.89929844896385	32.0	32.0	32.0	32.0	32.0
3	30.91565569147268	32.0	32.0	32.0	32.0	32.0
4	30.91340980841706	32.0	32.0	32.0	32.0	32.0
5	30.87266615988626	32.0	32.0	32.0	32.0	32.0
6	34.33721434040795	36.0	36.0	36.0	32.0	36.0
7	34.31662380087519	36.0	36.0	36.0	32.0	36.0
8	34.28738161118493	36.0	36.0	36.0	32.0	36.0
9	34.43800869652903	36.0	36.0	36.0	32.0	36.0
10	34.16346743067876	36.0	36.0	36.0	32.0	36.0
11	34.37630938278801	36.0	36.0	36.0	32.0	36.0
12	34.23125808235329	36.0	36.0	36.0	32.0	36.0
13	34.328629177475456	36.0	36.0	36.0	32.0	36.0
14	34.21535212188438	36.0	36.0	36.0	32.0	36.0
15	34.1490186485585	36.0	36.0	36.0	32.0	36.0
16	34.146491856977114	36.0	36.0	36.0	32.0	36.0
17	34.063970406536136	36.0	36.0	36.0	32.0	36.0
18	34.071872413231404	36.0	36.0	36.0	32.0	36.0
19	34.07348417437826	36.0	36.0	36.0	32.0	36.0
20	34.0376051744791	36.0	36.0	36.0	32.0	36.0
21	34.00964978962334	36.0	36.0	36.0	32.0	36.0
22	34.002201835269034	36.0	36.0	36.0	32.0	36.0
23	33.95867818964151	36.0	36.0	36.0	32.0	36.0
24	33.93471453155872	36.0	36.0	36.0	32.0	36.0
25	33.352521915851554	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	12.0
4	22.0
5	29.0
6	140.0
7	49.0
8	189.0
9	242.0
10	283.0
11	109.0
12	210.0
13	205.0
14	358.0
15	849.0
16	1518.0
17	2016.0
18	2614.0
19	3338.0
20	4411.0
21	6034.0
22	8180.0
23	11712.0
24	16907.0
25	23670.0
26	33205.0
27	46355.0
28	64849.0
29	90839.0
30	127605.0
31	186788.0
32	288406.0
33	439609.0
34	938738.0
35	1310225.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.71687090568165	17.556841706772673	11.877908874794436	25.848378512751243
2	16.870995678671342	19.153947125643224	37.79771815672809	26.177339038957342
3	18.691990872293278	23.484618672792436	28.125902956753094	29.69748749816119
4	12.427128574242488	15.416619685611552	35.546268005056504	36.60998373508946
5	14.891132394865672	36.29442876021976	33.270544102531424	15.543894742383147
6	34.86756817336148	35.108806688072534	16.670531691681276	13.35309344688472
7	31.16029071067505	29.898759999102232	20.37407566579633	18.566873624426393
8	28.412003846462948	32.798107806269066	18.755164874144732	20.034723473123258
9	27.031998627517662	14.537420514786199	18.465957199725835	39.96462365797031
10	15.995521820453718	26.21930329327475	30.947474801231582	26.837700085039952
11	37.34336186935223	20.858115026229004	22.563817998479976	19.234705105938794
12	25.499341510924044	23.06402687067862	28.3450510478029	23.091580570594434
13	29.13775199905529	19.38732967904788	25.348015525631094	26.126902796265732
14	23.66216134926111	20.02629618777352	24.67614190627125	31.635400556694123
15	25.07091242173855	27.74583457953847	21.782926633882347	25.40032636484063
16	26.052009325198274	25.556060685862235	23.63447107774787	24.757458911191623
17	24.040296912950218	26.152633303414362	24.926389915293655	24.88067986834176
18	24.918200766176454	24.810299522983353	26.121083381711458	24.15041632912873
19	25.688832604535357	24.69714431142522	25.077534992976314	24.536488091063106
20	26.035964102436143	24.264097168506183	24.42470466574067	25.275234063317004
21	27.01959505801559	24.059100659969936	23.971274701743823	24.95002958027065
22	26.122671900904347	24.281650780977095	24.60296865556609	24.992708662552467
23	24.334823932503554	24.196031581220833	25.447907537566955	26.02123694870866
24	24.966932747960033	24.887199847631425	24.962607875758618	25.18325952864992
25	24.747774809779333	24.657394867472785	25.116142384450214	25.478687938297668
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	127.0
1	127.0
2	398.0
3	669.0
4	669.0
5	669.0
6	1715.5
7	2762.0
8	2762.0
9	2762.0
10	3208.0
11	3654.0
12	3654.0
13	3654.0
14	5266.5
15	6879.0
16	6879.0
17	6879.0
18	12275.5
19	17672.0
20	17672.0
21	17672.0
22	31831.0
23	45990.0
24	45990.0
25	45990.0
26	72044.0
27	98098.0
28	98098.0
29	98098.0
30	125088.5
31	152079.0
32	152079.0
33	152079.0
34	191688.5
35	231298.0
36	231298.0
37	231298.0
38	263830.0
39	296362.0
40	296362.0
41	296362.0
42	338659.0
43	380956.0
44	380956.0
45	380956.0
46	424663.0
47	468370.0
48	468370.0
49	468370.0
50	477114.5
51	485859.0
52	485859.0
53	485859.0
54	454272.0
55	422685.0
56	422685.0
57	422685.0
58	389386.0
59	356087.0
60	356087.0
61	356087.0
62	314250.0
63	272413.0
64	272413.0
65	272413.0
66	223368.0
67	174323.0
68	174323.0
69	174323.0
70	131846.5
71	89370.0
72	89370.0
73	89370.0
74	68774.0
75	48178.0
76	48178.0
77	48178.0
78	40553.5
79	32929.0
80	32929.0
81	32929.0
82	22375.0
83	11821.0
84	11821.0
85	11821.0
86	9142.0
87	6463.0
88	6463.0
89	6463.0
90	4453.5
91	2444.0
92	2444.0
93	2444.0
94	1599.5
95	755.0
96	755.0
97	755.0
98	1114.0
99	1473.0
100	1473.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03773150020666446
2	0.004931135856671273
3	0.0013020414902446618
4	0.0029088160952274364
5	0.007923060983190922
6	0.011773779433063432
7	0.021248209000375655
8	0.03340983057946941
9	0.04584848226287054
10	0.05540602086147497
11	0.052026253588925
12	0.061362168104083535
13	0.0626642095943282
14	0.06507437150180236
15	0.058093212873256514
16	0.0633290818446659
17	0.060780404885038046
18	0.07518597030902154
19	0.07299743248499327
20	0.07718058706003464
21	0.07152917293216418
22	0.07468731612126826
23	0.08003399713440061
24	0.0740501468813613
25	0.0753798913820367
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3609716.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.32449865459321
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.49606852752518	26.935735546555883
2	15.635543068972543	12.297197847592898
3	6.276129182986207	7.404169008371826
4	3.053910645055623	4.803740202109508
5	1.7358981280635004	3.4131661800771993
6	1.0962616031891876	2.586596276381724
7	0.7084899076299672	1.9502707293571255
8	0.5091353904204736	1.6017195180476558
9	0.3673842081010223	1.3002479817468695
>10	1.882338484149235	13.125217459762286
>50	0.11512763463848326	3.130653461291441
>100	0.0935522275397088	7.639784734908313
>500	0.017870564187927474	4.828065874507803
>1k	0.012290427541051594	8.983435179289424
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4666	0.1292622466698211	No Hit
GAATAGGACCGCGGTTCTATTTTGT	4574	0.12671356971019326	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	4475	0.12397097167755025	No Hit
CTATTGGAGCTGGAATTACCGCGGC	4446	0.12316758437505887	No Hit
GTATCTGATCGTCTTCGAACCTCCG	4349	0.12048039236327733	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	4195	0.11621412875694377	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	4069	0.11272354944267084	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	3949	0.10939918819098235	No Hit
GTCCTATTCCATTATTCCTAGCTGC	3880	0.10748768047126145	No Hit
GTACATGGGGAATAATTGCAATCCC	3764	0.1042741312612959	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	3731	0.10335993191708157	No Hit
GAACTACGACGGTATCTGATCGTCT	3709	0.10275046568760533	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.7703010430737487E-5	1.3851505215368743E-4
2	0.0	0.0	0.0	2.7703010430737487E-5	1.3851505215368743E-4
3	0.0	0.0	0.0	2.7703010430737487E-5	1.3851505215368743E-4
4	0.0	0.0	0.0	2.7703010430737487E-5	1.3851505215368743E-4
5	0.0	0.0	0.0	2.7703010430737487E-5	1.3851505215368743E-4
6	0.0	0.0	0.0	2.7703010430737487E-5	3.601391355995873E-4
7	0.0	0.0	0.0	2.7703010430737487E-5	3.601391355995873E-4
8	0.0	0.0	0.0	2.7703010430737487E-5	3.601391355995873E-4
9	0.0	0.0	0.0	2.7703010430737487E-5	3.601391355995873E-4
10	0.0	0.0	0.0	2.7703010430737487E-5	3.601391355995873E-4
11	0.0	0.0	0.0	2.7703010430737487E-5	3.878421460303248E-4
12	0.0	0.0	0.0	2.7703010430737487E-5	9.69605365075812E-4
13	0.0	0.0	0.0	2.7703010430737487E-5	0.0011081204172294994
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAC	25	0.0060426467	18.995068	1
GTATCAA	4610	0.0	12.999877	1
CCGTCGT	710	0.0	12.846007	9
CGTCGTA	750	0.0	12.793557	10
GGTATCA	1455	0.0	12.663377	1
GCGTTAT	350	0.0	12.211114	1
ATTTGCG	590	0.0	11.755746	16
CGAACGA	245	0.0	11.634148	16
CGCCAGT	515	0.0	11.623657	18
ACCGTCG	785	0.0	11.618843	8
CGTTATT	360	0.0	11.605844	2
TTTGCGC	595	0.0	11.497594	17
CGGCTAA	75	2.0738585E-4	11.400831	9
CGCATCG	510	0.0	11.363732	13
GTCCTAG	370	0.0	11.294364	1
CGGACAT	405	0.0	11.25774	5
TCGCGTA	305	0.0	11.213934	9
GTCGTAG	800	0.0	11.163159	11
TTGCGCG	615	0.0	11.124151	18
CGCGCTA	130	3.2647222E-8	10.962946	16
>>END_MODULE