FastQCFastQC Report
Thu 2 Feb 2017
SRR4063865_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063865_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3609716
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT45920.12721222389794654No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA43970.12181013686395274No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC43100.11939997495647857No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA41930.11615872273608228No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT38480.10660118413747786No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC37740.10455116136560327No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC37290.10330452589622009No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG37230.10313830783363566No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA37010.10252884160415945No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA36150.10014638270711602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48350.026.1622241
GGTATCA19950.022.2746931
CGCAATA12450.019.43930636
TAGGACG13300.019.0211774
TTAGGAC10850.018.8557783
CTAGCGG13050.018.37618629
CGTCGTA12000.018.14994410
ATACCGT12250.018.1377126
ATACGAA13000.018.10738440
TCTAGCG13400.017.89596228
TAGCGGC13450.017.8306730
TACCGTC12100.017.8171377
TACGAAT13550.017.53475641
AACGCAG73250.017.3887426
AATACGA13800.017.37699739
TCAACGC72650.017.350434
CAATACG13950.017.34857738
GTATTAC3300.017.332561
ACCGTCG11950.017.3049058
TAGGACA7850.017.0943034
Produced by FastQC (version 0.11.5)