##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063865_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3609716
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15080216837003	32.0	32.0	32.0	32.0	32.0
2	30.983552168647062	32.0	32.0	32.0	32.0	32.0
3	31.24036433891198	32.0	32.0	32.0	32.0	32.0
4	31.39716891855204	32.0	32.0	32.0	32.0	32.0
5	31.264618601574195	32.0	32.0	32.0	32.0	32.0
6	34.85188696285248	36.0	36.0	36.0	32.0	36.0
7	34.890513270296054	36.0	36.0	36.0	36.0	36.0
8	34.79559056723576	36.0	36.0	36.0	32.0	36.0
9	34.99376848483371	36.0	36.0	36.0	36.0	36.0
10	34.749325154665904	36.0	36.0	36.0	32.0	36.0
11	34.997534986131875	36.0	36.0	36.0	36.0	36.0
12	34.859278680095606	36.0	36.0	36.0	32.0	36.0
13	34.936561491264136	36.0	36.0	36.0	32.0	36.0
14	34.85840021763485	36.0	36.0	36.0	32.0	36.0
15	34.801595194746625	36.0	36.0	36.0	32.0	36.0
16	34.82798729872378	36.0	36.0	36.0	32.0	36.0
17	34.77584303031042	36.0	36.0	36.0	32.0	36.0
18	34.788489731602155	36.0	36.0	36.0	32.0	36.0
19	34.79106472642169	36.0	36.0	36.0	32.0	36.0
20	34.78511301166075	36.0	36.0	36.0	32.0	36.0
21	34.78477364978298	36.0	36.0	36.0	32.0	36.0
22	34.76255084887565	36.0	36.0	36.0	32.0	36.0
23	34.6978532383157	36.0	36.0	36.0	32.0	36.0
24	34.66881605090262	36.0	36.0	36.0	32.0	36.0
25	34.63471863160426	36.0	36.0	36.0	32.0	36.0
26	34.558012320082796	36.0	36.0	36.0	32.0	36.0
27	34.5524492785582	36.0	36.0	36.0	32.0	36.0
28	34.5263951513083	36.0	36.0	36.0	32.0	36.0
29	34.490451326364735	36.0	36.0	36.0	32.0	36.0
30	34.47174680778211	36.0	36.0	36.0	32.0	36.0
31	34.47480466607345	36.0	36.0	36.0	32.0	36.0
32	34.42286927835874	36.0	36.0	36.0	32.0	36.0
33	34.40216100103166	36.0	36.0	36.0	32.0	36.0
34	34.39875048341753	36.0	36.0	36.0	32.0	36.0
35	34.356887910295434	36.0	36.0	36.0	32.0	36.0
36	34.322792430207805	36.0	36.0	36.0	32.0	36.0
37	34.3201484548923	36.0	36.0	36.0	32.0	36.0
38	34.26877100580766	36.0	36.0	36.0	32.0	36.0
39	34.26967523206812	36.0	36.0	36.0	32.0	36.0
40	34.25335871298462	36.0	36.0	36.0	32.0	36.0
41	34.239208292286705	36.0	36.0	36.0	32.0	36.0
42	34.15690209423678	36.0	36.0	36.0	32.0	36.0
43	34.167523705466024	36.0	36.0	36.0	32.0	36.0
44	34.08013400500206	36.0	36.0	36.0	32.0	36.0
45	34.05890186374773	36.0	36.0	36.0	32.0	36.0
46	34.042068960549805	36.0	36.0	36.0	32.0	36.0
47	34.02686582545552	36.0	36.0	36.0	32.0	36.0
48	33.979382865577236	36.0	36.0	36.0	32.0	36.0
49	33.96078611170519	36.0	36.0	36.0	32.0	36.0
50	33.25953482213005	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	4.0
18	4.0
19	7.0
20	30.0
21	125.0
22	346.0
23	1109.0
24	2820.0
25	6432.0
26	13755.0
27	25523.0
28	43616.0
29	70536.0
30	106527.0
31	161289.0
32	245664.0
33	409616.0
34	808895.0
35	1713418.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.54938411839998	18.595939558825975	12.610556703772863	26.244119619001182
2	16.07880650535695	20.087946318006306	36.96091810348888	26.872329073147867
3	18.034740177260726	24.158803848839977	28.390610771887275	29.41584520201202
4	12.22187562678061	16.22684443873147	35.71591227675529	35.83536765773263
5	14.874743608638463	36.44760418825193	32.6967273879718	15.980924815137811
6	35.0870417870944	35.554254740625474	16.226099487963413	13.132603984316715
7	31.149735879498554	30.01923142984102	20.2199840652284	18.611048625432026
8	28.47985824923623	32.955141069269715	18.43230880213291	20.132691879361147
9	27.116621727305944	14.272016985443859	18.183528230626603	40.4278330566236
10	15.90674305749286	26.44088137379377	30.778575293176715	26.873800275536663
11	37.74516824735471	20.726954898101376	22.310182382819697	19.217694471724222
12	25.556263522554445	23.03999490263761	28.18968836831768	23.214053206490274
13	29.348679765265747	19.355076420499326	25.229333526147197	26.06691028808773
14	23.78380744370459	19.83240749667286	24.55508866645169	31.82869639317086
15	25.254360173174085	27.677517428087235	21.599053842490484	25.469068556248192
16	26.25574046252815	25.5878701813982	23.36706575064902	24.78932360542463
17	24.309419355982577	26.09202496816924	24.6663449423722	24.932210733475983
18	25.195992470298815	24.749307768917404	25.875438574620365	24.179261186163412
19	25.941410887009088	24.669648434848735	24.864760791364414	24.524179886777763
20	26.288315217767703	24.071258097983346	24.367654330906074	25.27277235334287
21	27.293449124529463	23.92636983075677	23.88736399207029	24.892817052643476
22	26.24936629258306	24.207540217912243	24.571641489205504	24.971452000299195
23	24.505172326384724	24.065300880324656	25.4119812471483	26.01754554614232
24	25.242824417605085	24.78190058085047	24.819742987450226	25.155532014094216
25	25.07427325243154	24.436564935489482	25.092557604018566	25.39660420806041
26	24.808057525255954	25.351948791329654	25.291414845915483	24.54857883749891
27	25.528783685320168	24.732858476635332	24.469692562148325	25.268665275896176
28	24.706517589551822	24.545806638212802	25.347643746401943	25.400032025833436
29	24.82859060741078	24.492157512966	25.265421720158255	25.41383015946497
30	24.568358657511066	24.67028147763987	25.632938219644647	25.12842164520442
31	25.63627387204484	24.45019354738614	24.401737438259328	25.51179514230969
32	25.16329285722399	24.679613000707278	24.249733836174954	25.90736030589378
33	24.428732469128516	24.58747753535679	25.134967936544083	25.848822058970605
34	25.464792821624666	24.440391077285444	25.19379469135108	24.90102140973881
35	25.84436477433693	24.42293579328719	25.154511280590935	24.578188151784943
36	24.65387982623343	24.95979985814974	24.831746752958907	25.554573562657918
37	25.86878191840966	24.638089491505138	24.47358345564626	25.019545134438943
38	25.040726358764314	24.50640761377892	24.945781303332137	25.507084724124635
39	25.781492844292085	24.28247606498953	24.270646387959108	25.66538470275928
40	25.946219388812363	24.678193985640625	24.887301406128277	24.48828521941873
41	24.88471028107808	24.92440994879768	25.42925785169667	24.761621918427572
42	26.21206639291989	25.135289675321403	24.640423396982875	24.01222053477583
43	25.236143862697467	24.27990527650219	25.031692700484033	25.452258160316305
44	24.874621676466717	24.806249870140665	24.49062588737213	25.82850256602049
45	24.998095392178836	25.11550234412205	24.634682368207372	25.251719895491743
46	25.009993935765657	24.667831494624608	24.64755273930801	25.674621830301724
47	25.47553113212147	24.444571880693076	25.02357570599682	25.056321281188637
48	26.09862688088784	25.195274353714264	23.90327080213815	24.802827963259748
49	25.18928155288165	25.086309063739886	24.315275887039984	25.409133496338477
50	25.255709466506982	25.311670369228374	24.169541588785435	25.26307857547921
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	16.0
2	26.0
3	85.5
4	145.0
5	172.0
6	199.0
7	204.0
8	209.0
9	283.5
10	358.0
11	604.0
12	850.0
13	1631.0
14	2412.0
15	3321.5
16	4231.0
17	4601.0
18	4971.0
19	5185.5
20	5400.0
21	6318.0
22	7236.0
23	8511.0
24	9786.0
25	13175.5
26	16565.0
27	21977.0
28	27389.0
29	33544.5
30	39700.0
31	46646.0
32	53592.0
33	63948.5
34	74305.0
35	88832.5
36	103360.0
37	121124.5
38	138889.0
39	150239.5
40	161590.0
41	175472.5
42	189355.0
43	191845.5
44	194336.0
45	213376.0
46	232416.0
47	250310.5
48	268205.0
49	275958.5
50	283712.0
51	273390.5
52	263069.0
53	261066.0
54	259063.0
55	261605.5
56	264148.0
57	258055.5
58	251963.0
59	229861.0
60	207759.0
61	183482.5
62	159206.0
63	138064.0
64	116922.0
65	99838.5
66	82755.0
67	71352.5
68	59950.0
69	54853.0
70	49756.0
71	38065.5
72	26375.0
73	22445.0
74	18515.0
75	14282.0
76	10049.0
77	8906.0
78	7763.0
79	6522.0
80	5281.0
81	4298.0
82	3315.0
83	2784.0
84	2253.0
85	1634.5
86	1016.0
87	723.5
88	431.0
89	352.5
90	274.0
91	235.5
92	197.0
93	159.0
94	121.0
95	128.0
96	135.0
97	101.0
98	67.0
99	78.5
100	90.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007091970670268797
2	3.878421460303248E-4
3	1.3851505215368743E-4
4	0.0
5	0.0
6	3.601391355995873E-4
7	0.0
8	0.0
9	0.001025011385937287
10	9.141993442143371E-4
11	2.7703010430737487E-5
12	1.6621806258442493E-4
13	4.7095117732253735E-4
14	2.216240834458999E-4
15	0.004681808762794636
16	9.141993442143371E-4
17	0.0
18	3.0473311473811236E-4
19	2.7703010430737487E-5
20	5.817632190454872E-4
21	0.0
22	1.6621806258442493E-4
23	6.925752607684372E-4
24	2.493270938766374E-4
25	0.001994616751013099
26	0.0047372147836561105
27	0.005706820148731922
28	0.0036013913559958736
29	0.0022993498657512116
30	0.003379767272549973
31	0.007313594753714697
32	0.0026871920117815364
33	0.00429396661676431
34	0.0026871920117815364
35	0.0030196281369503863
36	0.0076460308788835465
37	0.003379767272549973
38	0.007202782711991747
39	0.004155451564610623
40	0.002437864917904899
41	0.0031581431891040735
42	0.0014959625632598244
43	0.0014405565423983493
44	0.001135823427660237
45	0.001357447511106137
46	7.479812816299122E-4
47	0.0018838047092901494
48	0.0014128535319676118
49	3.878421460303248E-4
50	0.0014682595528290867
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3609716.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.922308830726244
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.23576831566423	30.146813386082773
2	15.178189773029038	13.029658978586461
3	5.861422378999255	7.547573446162084
4	2.8496645672662817	4.892567304807248
5	1.6335329271838366	3.505750239287244
6	0.9573797669279124	2.465577001462114
7	0.6326808290246932	1.9009285354274454
8	0.4484678656703452	1.5399420984767365
9	0.33649439322928243	1.299880463939559
>10	1.6718357539780997	12.60528206909658
>50	0.0952632770673543	2.8387381975763457
>100	0.07605969897015272	6.854900261488127
>500	0.01441167755569537	4.3428390695555725
>1k	0.00882877543390002	7.0295489480517865
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	4592	0.12721222389794654	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	4397	0.12181013686395274	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	4310	0.11939997495647857	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	4193	0.11615872273608228	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	3848	0.10660118413747786	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	3774	0.10455116136560327	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	3729	0.10330452589622009	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	3723	0.10313830783363566	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	3701	0.10252884160415945	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	3615	0.10014638270711602	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6621806258442493E-4
2	0.0	0.0	0.0	0.0	1.6621806258442493E-4
3	0.0	0.0	0.0	0.0	1.6621806258442493E-4
4	0.0	0.0	0.0	0.0	1.6621806258442493E-4
5	0.0	0.0	0.0	0.0	1.6621806258442493E-4
6	0.0	0.0	0.0	0.0	2.493270938766374E-4
7	0.0	0.0	0.0	0.0	2.493270938766374E-4
8	0.0	0.0	0.0	2.7703010430737487E-5	2.493270938766374E-4
9	0.0	0.0	0.0	2.7703010430737487E-5	2.493270938766374E-4
10	0.0	0.0	0.0	2.7703010430737487E-5	2.7703010430737485E-4
11	0.0	0.0	0.0	2.7703010430737487E-5	2.7703010430737485E-4
12	0.0	0.0	0.0	2.7703010430737487E-5	0.001025011385937287
13	0.0	0.0	0.0	2.7703010430737487E-5	0.0013020414902446618
14	0.0	0.0	0.0	2.7703010430737487E-5	0.0013020414902446618
15	0.0	0.0	0.0	2.7703010430737487E-5	0.0015236655736905619
16	0.0	0.0	0.0	2.7703010430737487E-5	0.0016067746049827742
17	0.0	0.0	0.0	5.540602086147497E-5	0.0016344776154135117
18	0.0	0.0	0.0	5.540602086147497E-5	0.0017452896571364617
19	0.0	0.0	0.0	5.540602086147497E-5	0.0017452896571364617
20	0.0	0.0	0.0	5.540602086147497E-5	0.002050022771874574
21	0.0	0.0	0.0	5.540602086147497E-5	0.002105428792736049
22	0.0	0.0	0.0	5.540602086147497E-5	0.002160834813597524
23	0.0	0.0	0.0	5.540602086147497E-5	0.002216240834458999
24	0.0	0.0	0.0	1.6621806258442493E-4	0.002216240834458999
25	0.0	0.0	0.0	2.216240834458999E-4	0.002216240834458999
26	0.0	0.0	0.0	2.7703010430737485E-4	0.002271646855320474
27	0.0	0.0	0.0	3.601391355995873E-4	0.002327052876181949
28	0.0	0.0	0.0	5.817632190454873E-4	0.002327052876181949
29	0.0	0.0	0.0	8.587933233528621E-4	0.002327052876181949
30	0.0	0.0	0.0	0.0015790715945520368	0.0023547558866126865
31	0.0	0.0	0.0	0.0039338274811647235	0.002382458897043424
32	0.0	0.0	0.0	0.007091970670268797	0.0024101619074741614
33	0.0	0.0	0.0	0.010277816869803608	0.0024655679283356363
34	0.0	0.0	0.0	0.013823802204938005	0.0026040829804893236
35	0.0	0.0	0.0	0.018450204946871166	0.0026040829804893236
36	0.0	0.0	0.0	0.02545906658584775	0.0026040829804893236
37	0.0	0.0	0.0	0.037011221935465285	0.0026317859909200615
38	0.0	0.0	0.0	0.053439107120892615	0.0026871920117815364
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4835	0.0	26.162224	1
GGTATCA	1995	0.0	22.274693	1
CGCAATA	1245	0.0	19.439306	36
TAGGACG	1330	0.0	19.021177	4
TTAGGAC	1085	0.0	18.855778	3
CTAGCGG	1305	0.0	18.376186	29
CGTCGTA	1200	0.0	18.149944	10
ATACCGT	1225	0.0	18.137712	6
ATACGAA	1300	0.0	18.107384	40
TCTAGCG	1340	0.0	17.895962	28
TAGCGGC	1345	0.0	17.83067	30
TACCGTC	1210	0.0	17.817137	7
TACGAAT	1355	0.0	17.534756	41
AACGCAG	7325	0.0	17.388742	6
AATACGA	1380	0.0	17.376997	39
TCAACGC	7265	0.0	17.35043	4
CAATACG	1395	0.0	17.348577	38
GTATTAC	330	0.0	17.33256	1
ACCGTCG	1195	0.0	17.304905	8
TAGGACA	785	0.0	17.094303	4
>>END_MODULE