FastQCFastQC Report
Thu 2 Feb 2017
SRR4063100_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063100_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences723228
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA22550.31179655654924865No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT19390.2681035579374692No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC16940.2342276571150453No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT13240.18306813342403777No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC13160.18196198156044843No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT13120.18140890562865375No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC10930.15112799836289526No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC10920.15098972937994656No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA10880.1504366534481519No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG10500.14518243209610246No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT10450.14449108718135914No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG10390.14366147328366713No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG10090.13951340379520705No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG9960.13771590701687433No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG9580.13246168566482494No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA9090.12568650550034013No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG8620.1191878633017527No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC8390.11600767669393332No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8280.11448671788149795No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG8050.11130653127367855No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT7870.10881768958060252No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT7790.10771153771701317No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT7610.10522269602393712No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC7600.10508442704098846No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC7540.10425481314329645No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA7480.10342519924560444No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT7270.10052155060368238No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTC401.5941329E-423.9969397
ATACTTA400.00448700319.9960676
TAGCTAT507.138501E-419.2214781
AACGAAT1600.018.00019531
GTCATAC450.00879038917.7976631
ACCGTCT450.00885016317.776748
CCAACGA1550.017.54857629
CGCTTAC550.001363883817.45473318
AAGACGG2850.017.4000875
CCGTCGT1750.017.3704179
CAAGACG3050.017.3068754
GATATAC2500.017.299331
TCTAAAC652.102203E-417.227383
AGACCGT652.102203E-417.227386
TGAATAG652.102203E-417.227385
GTCCTAT2700.017.2044071
CCTATTC2450.016.9762543
TATACTG854.9881055E-616.9378475
TCTAGAT1800.016.8949132
CGTCGTA1800.016.8890710
Produced by FastQC (version 0.11.5)