##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063100_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	723228
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.19610275044661	32.0	32.0	32.0	32.0	32.0
2	30.772795024528918	32.0	32.0	32.0	32.0	32.0
3	30.84000204638095	32.0	32.0	32.0	32.0	32.0
4	30.908452106389685	32.0	32.0	32.0	32.0	32.0
5	30.796130680781165	32.0	32.0	32.0	32.0	32.0
6	34.439569541002285	36.0	36.0	36.0	32.0	36.0
7	34.37549292892421	36.0	36.0	36.0	32.0	36.0
8	34.35627077491469	36.0	36.0	36.0	32.0	36.0
9	34.51700985028234	36.0	36.0	36.0	32.0	36.0
10	34.20862992030176	36.0	36.0	36.0	32.0	36.0
11	34.502068503984916	36.0	36.0	36.0	32.0	36.0
12	34.301568799880535	36.0	36.0	36.0	32.0	36.0
13	34.40150132461686	36.0	36.0	36.0	32.0	36.0
14	34.28438887874916	36.0	36.0	36.0	32.0	36.0
15	34.21581990741509	36.0	36.0	36.0	32.0	36.0
16	34.237801910324265	36.0	36.0	36.0	32.0	36.0
17	34.13699552561571	36.0	36.0	36.0	32.0	36.0
18	34.182562068946446	36.0	36.0	36.0	32.0	36.0
19	34.16110963624196	36.0	36.0	36.0	32.0	36.0
20	34.15171564154043	36.0	36.0	36.0	32.0	36.0
21	34.122262965482534	36.0	36.0	36.0	32.0	36.0
22	34.094126610142304	36.0	36.0	36.0	32.0	36.0
23	34.013898798166004	36.0	36.0	36.0	32.0	36.0
24	33.98419447255914	36.0	36.0	36.0	32.0	36.0
25	33.94878655140564	36.0	36.0	36.0	32.0	36.0
26	33.9012690327255	36.0	36.0	36.0	32.0	36.0
27	33.879206833806215	36.0	36.0	36.0	32.0	36.0
28	33.8645102236086	36.0	36.0	36.0	32.0	36.0
29	33.82274192923946	36.0	36.0	36.0	32.0	36.0
30	33.7624732449518	36.0	36.0	36.0	32.0	36.0
31	33.803432665770686	36.0	36.0	36.0	32.0	36.0
32	33.7553689846079	36.0	36.0	36.0	32.0	36.0
33	33.70575392545643	36.0	36.0	36.0	27.0	36.0
34	33.71490041867848	36.0	36.0	36.0	27.0	36.0
35	33.64807501921939	36.0	36.0	36.0	27.0	36.0
36	33.596930705116506	36.0	36.0	36.0	27.0	36.0
37	33.582955859009886	36.0	36.0	36.0	27.0	36.0
38	32.927368685946895	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	1.0
10	6.0
11	7.0
12	2.0
13	2.0
14	53.0
15	152.0
16	207.0
17	268.0
18	315.0
19	444.0
20	698.0
21	995.0
22	1532.0
23	2274.0
24	3363.0
25	5151.0
26	7631.0
27	11160.0
28	15712.0
29	21216.0
30	28811.0
31	38460.0
32	51622.0
33	75408.0
34	159939.0
35	297795.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.55354529861522	16.621559325037275	11.036186009294651	25.78870936705286
2	16.84390957222366	19.431098002160947	36.80417690995544	26.920815515659953
3	19.649477493573247	23.273765157664602	27.879040142487927	29.197717206274227
4	12.479069040708728	14.576382272946622	35.39174597731506	37.55280270902959
5	14.519231168084158	36.43613969058124	33.5467287587438	15.497900382590805
6	35.411979917785146	34.77552690925532	16.24884026730197	13.56365290565756
7	30.798171530969487	29.960123225317602	20.350290641402157	18.891414602310753
8	29.100251097861797	32.29372919040298	19.187831156045707	19.41818855568952
9	27.400010508471762	13.941147027346915	18.14688631216245	40.51195615201887
10	16.15543731955798	25.878151167549753	30.93647334712344	27.029938165768836
11	38.82422402159244	20.620395566469767	21.127021526072433	19.428358885865357
12	24.773164087885952	23.470223033420446	28.116729580619737	23.639883298073865
13	30.020812445980987	18.473846153846154	25.28622299049265	26.21911840968021
14	23.905176359636204	19.461026919614522	24.184370389115443	32.44942633163383
15	25.83118046988665	26.610360298063252	22.31159448350639	25.246864748543707
16	26.786072348669755	24.98599850374134	23.467475734196977	24.760453413391932
17	24.334449844140256	25.433350943041354	25.167545769106304	25.064653443712086
18	25.961780444972966	23.599883847951435	26.476444641103996	23.961891065971596
19	25.936634890081777	24.937152054692234	24.72240428997724	24.40380876524875
20	25.661933892342752	23.656635102546517	25.34140688953295	25.34002411557778
21	27.239226477319082	23.838713468891086	23.60696087446504	25.31509917932479
22	25.729452874046398	24.233419388560783	24.708682082859156	25.328445654533667
23	24.18106064901177	23.615626395781852	25.27058289924374	26.93273005596264
24	24.72068912585619	25.292794132086144	24.795503921207988	25.19101282084968
25	25.32917346034222	24.03751076961799	24.77779667790994	25.855519092129846
26	24.315028709880846	24.97192627258345	25.749831281047054	24.963213736488655
27	25.833206576600247	24.380436135348535	24.74272992519048	25.043627362860736
28	24.607566580934208	23.974252606543324	25.636770928399567	25.7814098841229
29	24.198551079361525	24.797451182934875	25.726707404595672	25.27729033310793
30	24.753771720839424	24.649652729286473	25.97665135071231	24.619924199161794
31	25.086146340923797	24.645320442263976	24.47634639955309	25.79218681725914
32	24.683896125445948	24.67020658757156	24.602035454520312	26.043861832462177
33	24.312809577936896	24.02573697179885	25.376333380349074	26.28512006991518
34	25.02838320156982	24.83893138110377	25.521234506782235	24.611450910544168
35	26.226241315218836	24.202604328008142	25.531597114661235	24.039557242111783
36	24.553389921921028	25.353263800728516	24.849885356942337	25.243460920408122
37	26.11061483106043	24.740651420574757	24.81836611086374	24.33036763750107
38	24.755491333476222	24.587899361850702	25.039512711132005	25.61709659354107
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	33.0
1	49.5
2	66.0
3	66.0
4	218.0
5	370.0
6	370.0
7	497.0
8	624.0
9	607.0
10	590.0
11	590.0
12	727.5
13	865.0
14	1194.0
15	1523.0
16	1523.0
17	2247.0
18	2971.0
19	2971.0
20	3530.5
21	4090.0
22	4433.5
23	4777.0
24	4777.0
25	5762.0
26	6747.0
27	6747.0
28	8654.5
29	10562.0
30	12837.5
31	15113.0
32	15113.0
33	19548.5
34	23984.0
35	23984.0
36	26486.5
37	28989.0
38	33547.5
39	38106.0
40	38106.0
41	39749.0
42	41392.0
43	47662.5
44	53933.0
45	53933.0
46	57823.5
47	61714.0
48	61714.0
49	63819.5
50	65925.0
51	66360.5
52	66796.0
53	66796.0
54	63633.5
55	60471.0
56	60471.0
57	59521.5
58	58572.0
59	53043.5
60	47515.0
61	47515.0
62	46125.0
63	44735.0
64	37472.0
65	30209.0
66	30209.0
67	25336.0
68	20463.0
69	20463.0
70	16404.0
71	12345.0
72	9729.5
73	7114.0
74	7114.0
75	5339.5
76	3565.0
77	3565.0
78	3416.0
79	3267.0
80	2566.5
81	1866.0
82	1866.0
83	1891.5
84	1917.0
85	1917.0
86	1283.5
87	650.0
88	539.0
89	428.0
90	428.0
91	331.5
92	235.0
93	191.5
94	148.0
95	148.0
96	144.0
97	140.0
98	140.0
99	279.0
100	418.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12126189804598274
2	0.054892786230621604
3	0.010923249652944852
4	0.0023505727101273736
5	1.3826898294866903E-4
6	1.3826898294866903E-4
7	0.0
8	5.530759317946761E-4
9	2.7653796589733806E-4
10	5.530759317946761E-4
11	0.0016592277953840283
12	0.002488841693076042
13	0.01424170524371291
14	0.010370173721150176
15	0.022123037271787042
16	0.01258247744832888
17	0.019634195578711003
18	0.0052542213520494225
19	0.007466525079228127
20	0.006083835249741437
21	0.005945566266792768
22	0.006775180164484781
23	0.009264021857560825
24	0.015071319141404924
25	0.017836698800378303
26	0.01825150574922431
27	0.008019601011022804
28	0.006636911181536113
29	0.009540559823458162
30	0.0023505727101273736
31	0.005530759317946761
32	0.006636911181536113
33	0.009125752874612154
34	0.012305939482431543
35	0.01714535388563496
36	0.01576266405614827
37	0.009678828806406832
38	0.005945566266792768
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	723228.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.295281078797146
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.35123420930651	39.909926285065346
2	12.03197792400026	12.103034232429506
3	3.600485637993371	5.432623115491466
4	1.5765242287973196	3.171669168595804
5	0.8091709649519339	2.0348740561529515
6	0.5030300426631706	1.5180022432094091
7	0.3623290644393238	1.2756409497295376
8	0.2469470952749019	0.9936218854754952
9	0.19863469991098134	0.8991349257622796
>10	1.0656586367098067	10.13552408479679
>50	0.11580976497341962	4.086314658922294
>100	0.12410156241913035	12.46370595819417
>500	0.010503027554393012	3.5771050181366135
>1k	0.003593141005450241	2.3988234180384507
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2255	0.31179655654924865	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	1939	0.2681035579374692	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1694	0.2342276571150453	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1324	0.18306813342403777	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1316	0.18196198156044843	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1312	0.18140890562865375	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1093	0.15112799836289526	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1092	0.15098972937994656	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1088	0.1504366534481519	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1050	0.14518243209610246	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1045	0.14449108718135914	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1039	0.14366147328366713	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	1009	0.13951340379520705	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	996	0.13771590701687433	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	958	0.13246168566482494	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	909	0.12568650550034013	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	862	0.1191878633017527	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	839	0.11600767669393332	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	828	0.11448671788149795	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	805	0.11130653127367855	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	787	0.10881768958060252	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	779	0.10771153771701317	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	761	0.10522269602393712	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	760	0.10508442704098846	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	754	0.10425481314329645	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	748	0.10342519924560444	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	727	0.10052155060368238	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.3826898294866903E-4	0.0
10	0.0	0.0	0.0	1.3826898294866903E-4	0.0
11	0.0	0.0	0.0	4.1480694884600707E-4	0.0
12	0.0	0.0	0.0	4.1480694884600707E-4	0.0
13	0.0	0.0	0.0	4.1480694884600707E-4	0.0
14	0.0	0.0	0.0	4.1480694884600707E-4	0.0
15	0.0	0.0	0.0	4.1480694884600707E-4	0.0
16	0.0	0.0	0.0	5.530759317946761E-4	0.0
17	0.0	0.0	0.0	5.530759317946761E-4	0.0
18	0.0	0.0	0.0	8.296138976920141E-4	0.0
19	0.0	0.0	0.0	0.0012444208465380213	0.0
20	0.0	0.0	0.0	0.0013826898294866902	0.0
21	0.0	0.0	0.0	0.0016592277953840283	0.0
22	0.0	0.0	0.0	0.0024888416930760426	0.0
23	0.0	0.0	0.0	0.0034567245737167256	0.0
24	0.0	0.0	0.0	0.0052542213520494225	0.0
25	0.0	0.0	0.0	0.006083835249741437	0.0
26	0.0	0.0	0.0	0.008019601011022804	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGTC	40	1.5941329E-4	23.996939	7
ATACTTA	40	0.004487003	19.996067	6
TAGCTAT	50	7.138501E-4	19.221478	1
AACGAAT	160	0.0	18.000195	31
GTCATAC	45	0.008790389	17.797663	1
ACCGTCT	45	0.008850163	17.77674	8
CCAACGA	155	0.0	17.548576	29
CGCTTAC	55	0.0013638838	17.454733	18
AAGACGG	285	0.0	17.400087	5
CCGTCGT	175	0.0	17.370417	9
CAAGACG	305	0.0	17.306875	4
GATATAC	250	0.0	17.29933	1
TCTAAAC	65	2.102203E-4	17.22738	3
AGACCGT	65	2.102203E-4	17.22738	6
TGAATAG	65	2.102203E-4	17.22738	5
GTCCTAT	270	0.0	17.204407	1
CCTATTC	245	0.0	16.976254	3
TATACTG	85	4.9881055E-6	16.937847	5
TCTAGAT	180	0.0	16.894913	2
CGTCGTA	180	0.0	16.88907	10
>>END_MODULE