FastQCFastQC Report
Thu 2 Feb 2017
SRR4063100_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063100_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences723228
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA22860.31608289502065734No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC20830.2880142914820776No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT16750.23160054643902062No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC15220.21044539204787424No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT13130.18154717461160244No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT11400.15762664056148268No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC11190.15472299191956063No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA11120.15375510903891995No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC10880.1504366534481519No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG10390.14366147328366713No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG10350.14310839735187242No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT10300.1424170524371291No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG10050.13896032786341236No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG9910.13702456210213101No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA9100.12582477448328883No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG8990.12430381567085345No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC8580.11863478736995803No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA8370.11573113872803598No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG8350.11545460076213863No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG8190.11324229703495994No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC8130.1124126831372679No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA8020.11089172432483256No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT7990.11047691737598654No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC7510.10384000619445043No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT7490.1035634682285531No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT7410.10245731636496375No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG7380.10204250941611775No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT7270.10052155060368238No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG7260.1003832816207337No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT200.003753715431.99806226
CTAGCGG2800.023.42715529
TCTAGCG2750.023.2713228
TAGCGGC2850.022.45478230
CCAACGA1101.8189894E-1221.81686229
AACGAAT1203.6379788E-1219.9987931
GCCGGTC3250.019.6965648
ACCCGTT659.430814E-619.69111630
CCGGTCC3400.019.2969519
TAGGACG1257.2759576E-1219.2028224
GATATAC1950.018.8706531
AGCGGCG3550.018.47775531
CTTGCGC3300.018.426953
CGGTCCA3400.018.353110
TCTAGAT1850.018.162322
CGGTTTC802.7948881E-617.9989123
AGGTCGT802.7948881E-617.9989128
CGCAAGA2600.017.8463082
TCTTGCG3500.017.8287282
GACGTGA904.3513137E-717.781627
Produced by FastQC (version 0.11.5)