##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063100_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	723228
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.095310192636347	32.0	32.0	32.0	32.0	32.0
2	31.16463272992749	32.0	32.0	32.0	32.0	32.0
3	31.309303566786685	32.0	32.0	32.0	32.0	32.0
4	31.424209239686515	32.0	32.0	32.0	32.0	32.0
5	31.316732759240516	32.0	32.0	32.0	32.0	32.0
6	34.84340208067166	36.0	36.0	36.0	32.0	36.0
7	34.88472514891569	36.0	36.0	36.0	32.0	36.0
8	34.8105936163976	36.0	36.0	36.0	32.0	36.0
9	34.95587421947159	36.0	36.0	36.0	32.0	36.0
10	34.76258800820764	36.0	36.0	36.0	32.0	36.0
11	34.957967611873435	36.0	36.0	36.0	36.0	36.0
12	34.83665870237325	36.0	36.0	36.0	32.0	36.0
13	34.90756718489882	36.0	36.0	36.0	32.0	36.0
14	34.837587869938666	36.0	36.0	36.0	32.0	36.0
15	34.806451077668456	36.0	36.0	36.0	32.0	36.0
16	34.815607249719314	36.0	36.0	36.0	32.0	36.0
17	34.769733749246434	36.0	36.0	36.0	32.0	36.0
18	34.78240195346419	36.0	36.0	36.0	32.0	36.0
19	34.768981565979196	36.0	36.0	36.0	32.0	36.0
20	34.76000099553668	36.0	36.0	36.0	32.0	36.0
21	34.73795677158517	36.0	36.0	36.0	32.0	36.0
22	34.707957933044625	36.0	36.0	36.0	32.0	36.0
23	34.634615916419165	36.0	36.0	36.0	32.0	36.0
24	34.600854778852586	36.0	36.0	36.0	32.0	36.0
25	34.56928243928609	36.0	36.0	36.0	32.0	36.0
26	34.50035120321669	36.0	36.0	36.0	32.0	36.0
27	34.48010724142318	36.0	36.0	36.0	32.0	36.0
28	34.425345810726355	36.0	36.0	36.0	32.0	36.0
29	34.38456199151582	36.0	36.0	36.0	32.0	36.0
30	34.359734412937556	36.0	36.0	36.0	32.0	36.0
31	34.35915783127866	36.0	36.0	36.0	32.0	36.0
32	34.328345971118374	36.0	36.0	36.0	32.0	36.0
33	34.27974718899158	36.0	36.0	36.0	32.0	36.0
34	34.26404536328793	36.0	36.0	36.0	32.0	36.0
35	34.20148971002229	36.0	36.0	36.0	32.0	36.0
36	34.17805311741249	36.0	36.0	36.0	32.0	36.0
37	34.16013207453251	36.0	36.0	36.0	32.0	36.0
38	33.659852771186955	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	2.0
21	7.0
22	45.0
23	170.0
24	585.0
25	1260.0
26	2695.0
27	5295.0
28	9291.0
29	14700.0
30	22663.0
31	32347.0
32	46747.0
33	75643.0
34	176586.0
35	335191.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.62074305974236	17.962808294568926	11.793321644090202	26.62312700159851
2	15.911579750783986	20.37476425138407	36.130376589401955	27.583279408429984
3	18.647784654355195	23.98911546566228	28.20313925898887	29.159960620993658
4	12.051248015840095	15.429574076224926	35.42451343144901	37.09466447648598
5	14.17395344206806	36.764478145204556	33.138788874324554	15.922779538402828
6	35.60343349538458	35.1958165336519	15.791977080533387	13.408772890430127
7	30.661604171304447	30.02598077781941	20.307870143122422	19.004544907753722
8	29.0127750069482	32.56361577358689	18.870063840466585	19.553545378998326
9	27.266115227088573	13.782117760281418	17.883069366895374	41.068697645734645
10	15.920903626702284	26.105232301695615	30.920786096604207	27.05307797499789
11	38.98843187305204	20.56626575994823	20.964775703338244	19.48052666366148
12	24.474507277116697	23.53164847502717	28.082646365036656	23.91119788281948
13	30.027681526936256	18.377381344144155	25.19959182883359	26.395345300086003
14	23.818215002737723	19.40660483277749	24.047879783415464	32.72730038106932
15	25.732687340645	26.900230632663558	21.956008340385054	25.411073686306395
16	26.654941456912617	25.25662723235273	23.151067160010395	24.937364150724253
17	24.389957247230473	25.646822302233872	24.810709762343272	25.152510688192383
18	26.119287416969478	23.67427699148816	26.106981477487047	24.09945411405532
19	25.907459335092113	25.190811196469166	24.50734761375389	24.39438185468483
20	25.800024335341	23.662247589971628	25.113104028052014	25.424624046635362
21	27.390608191630278	23.98060910420809	23.33489116818256	25.293891535979075
22	25.71720121455475	24.341148296249592	24.523801622724783	25.417848866470877
23	24.173737982680404	23.55498342843948	25.09364279818093	27.177635790699185
24	24.700509382933184	25.318571736713736	24.614229537573213	25.366689342779868
25	25.56859741132452	23.968131720746044	24.419995381809585	26.04327548611985
26	24.410974132638668	25.147533004806228	25.402915816312422	25.038577046242676
27	25.9713396052144	24.365068830299712	24.519791822219272	25.143799742266616
28	24.545371584064775	24.054378425614054	25.56192514670339	25.838324843617784
29	24.33271388828972	24.663729833468835	25.634101555802598	25.369454722438846
30	24.824260122672243	24.602891480971422	25.694939908300015	24.877908488056324
31	25.048255875049087	24.600264370295395	24.329533701681903	26.02194605297361
32	24.81803801843955	24.629715663663465	24.357464036237538	26.19478228165945
33	24.367281134026893	24.034052885120598	25.275570083016696	26.323095897835813
34	25.132461685664826	24.675206159053577	25.411903300204088	24.780428855077513
35	26.290187879894027	24.312664885762167	25.048808950980877	24.34833828336292
36	24.58035363675079	25.289258712328618	24.62086644875475	25.509521202165846
37	26.261151393474808	24.736182780533937	24.628332973833977	24.374332852157274
38	25.030557445231654	24.26344112783244	24.87541964636325	25.830581780572654
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	20.0
1	28.5
2	37.0
3	37.0
4	139.0
5	241.0
6	241.0
7	335.0
8	429.0
9	457.0
10	485.0
11	485.0
12	633.0
13	781.0
14	1086.5
15	1392.0
16	1392.0
17	2025.0
18	2658.0
19	2658.0
20	3182.5
21	3707.0
22	4174.5
23	4642.0
24	4642.0
25	5635.0
26	6628.0
27	6628.0
28	8635.0
29	10642.0
30	12947.0
31	15252.0
32	15252.0
33	19662.5
34	24073.0
35	24073.0
36	26589.0
37	29105.0
38	32977.5
39	36850.0
40	36850.0
41	38805.5
42	40761.0
43	47014.0
44	53267.0
45	53267.0
46	57949.5
47	62632.0
48	62632.0
49	65223.5
50	67815.0
51	67489.0
52	67163.0
53	67163.0
54	63895.5
55	60628.0
56	60628.0
57	59907.5
58	59187.0
59	53383.5
60	47580.0
61	47580.0
62	46129.5
63	44679.0
64	37680.0
65	30681.0
66	30681.0
67	25813.5
68	20946.0
69	20946.0
70	16869.5
71	12793.0
72	9959.5
73	7126.0
74	7126.0
75	5263.5
76	3401.0
77	3401.0
78	3330.5
79	3260.0
80	2561.0
81	1862.0
82	1862.0
83	1767.0
84	1672.0
85	1672.0
86	1066.5
87	461.0
88	379.0
89	297.0
90	297.0
91	168.5
92	40.0
93	30.5
94	21.0
95	21.0
96	13.5
97	6.0
98	6.0
99	7.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007743063045125465
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.3826898294866903E-4
8	0.0026271106760247112
9	0.004148069488460071
10	0.001244420846538021
11	0.004701145420254747
12	2.7653796589733806E-4
13	2.7653796589733806E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	2.7653796589733806E-4
22	0.0
23	1.3826898294866903E-4
24	0.0
25	1.3826898294866903E-4
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	723228.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.33245068450671
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.88378051210691	37.64313980724353
2	12.873364887693285	12.444025471561918
3	3.899149492773566	5.653663517129932
4	1.6387826460047838	3.1682552568260656
5	0.9029501683458973	2.1820897241072554
6	0.5411645376981824	1.5693484998300762
7	0.3839196490224381	1.298904425223308
8	0.26093296798630256	1.008922384572795
9	0.19203600395116424	0.8353413621557445
>10	1.1462231347202436	10.408036098726162
>50	0.13108633730703462	4.4519571923641355
>100	0.13223616607735525	13.330719223323207
>500	0.010636387271435784	3.581488867784075
>1k	0.003737109041315276	2.424108169151909
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2286	0.31608289502065734	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	2083	0.2880142914820776	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	1675	0.23160054643902062	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1522	0.21044539204787424	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1313	0.18154717461160244	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1140	0.15762664056148268	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1119	0.15472299191956063	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1112	0.15375510903891995	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1088	0.1504366534481519	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1039	0.14366147328366713	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	1035	0.14310839735187242	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1030	0.1424170524371291	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1005	0.13896032786341236	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	991	0.13702456210213101	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	910	0.12582477448328883	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	899	0.12430381567085345	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	858	0.11863478736995803	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	837	0.11573113872803598	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	835	0.11545460076213863	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	819	0.11324229703495994	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	813	0.1124126831372679	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	802	0.11089172432483256	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	799	0.11047691737598654	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	751	0.10384000619445043	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	749	0.1035634682285531	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	741	0.10245731636496375	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	738	0.10204250941611775	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	727	0.10052155060368238	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	726	0.1003832816207337	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3826898294866903E-4
2	0.0	0.0	0.0	0.0	1.3826898294866903E-4
3	0.0	0.0	0.0	0.0	1.3826898294866903E-4
4	0.0	0.0	0.0	0.0	1.3826898294866903E-4
5	0.0	0.0	0.0	0.0	1.3826898294866903E-4
6	0.0	0.0	0.0	0.0	1.3826898294866903E-4
7	0.0	0.0	0.0	0.0	1.3826898294866903E-4
8	0.0	0.0	0.0	0.0	1.3826898294866903E-4
9	0.0	0.0	0.0	0.0	1.3826898294866903E-4
10	0.0	0.0	0.0	0.0	1.3826898294866903E-4
11	0.0	0.0	0.0	1.3826898294866903E-4	1.3826898294866903E-4
12	0.0	0.0	0.0	1.3826898294866903E-4	1.3826898294866903E-4
13	0.0	0.0	0.0	1.3826898294866903E-4	1.3826898294866903E-4
14	0.0	0.0	0.0	1.3826898294866903E-4	1.3826898294866903E-4
15	0.0	0.0	0.0	1.3826898294866903E-4	1.3826898294866903E-4
16	0.0	0.0	0.0	2.7653796589733806E-4	1.3826898294866903E-4
17	0.0	0.0	0.0	2.7653796589733806E-4	1.3826898294866903E-4
18	0.0	0.0	0.0	4.1480694884600707E-4	2.7653796589733806E-4
19	0.0	0.0	0.0	8.296138976920141E-4	2.7653796589733806E-4
20	0.0	0.0	0.0	9.678828806406832E-4	4.1480694884600707E-4
21	0.0	0.0	0.0	0.0012444208465380213	4.1480694884600707E-4
22	0.0	0.0	0.0	0.0017974967783326973	4.1480694884600707E-4
23	0.0	0.0	0.0	0.0026271106760247112	4.1480694884600707E-4
24	0.0	0.0	0.0	0.004148069488460071	4.1480694884600707E-4
25	1.3826898294866903E-4	0.0	0.0	0.004977683386152085	4.1480694884600707E-4
26	1.3826898294866903E-4	0.0	0.0	0.006498642198587444	4.1480694884600707E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGATT	20	0.0037537154	31.998062	26
CTAGCGG	280	0.0	23.427155	29
TCTAGCG	275	0.0	23.27132	28
TAGCGGC	285	0.0	22.454782	30
CCAACGA	110	1.8189894E-12	21.816862	29
AACGAAT	120	3.6379788E-12	19.99879	31
GCCGGTC	325	0.0	19.696564	8
ACCCGTT	65	9.430814E-6	19.691116	30
CCGGTCC	340	0.0	19.296951	9
TAGGACG	125	7.2759576E-12	19.202822	4
GATATAC	195	0.0	18.870653	1
AGCGGCG	355	0.0	18.477755	31
CTTGCGC	330	0.0	18.42695	3
CGGTCCA	340	0.0	18.3531	10
TCTAGAT	185	0.0	18.16232	2
CGGTTTC	80	2.7948881E-6	17.99891	23
AGGTCGT	80	2.7948881E-6	17.99891	28
CGCAAGA	260	0.0	17.846308	2
TCTTGCG	350	0.0	17.828728	2
GACGTGA	90	4.3513137E-7	17.78162	7
>>END_MODULE