FastQCFastQC Report
Thu 2 Feb 2017
SRR4063099_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063099_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1251597
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT45360.3624169760713712No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT29960.23937417555331308No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG20110.1606747219752045No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG18100.14461523956992547No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG17820.1423780977423244No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA17650.1410198330612809No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA16450.1314320823715621No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC16270.12999391976810426No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC16220.12959443015603264No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG16210.12951453223361833No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT15790.12615881949221674No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT15520.12400157558703001No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC15390.12296290259564381No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG15270.12200412752667192No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA15260.1219242296042576No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT15060.12032627115597114No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT14510.11593188542318333No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG13520.10802199110416533No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC12930.10330801368172024No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA12880.10290852406964861No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC12660.10115076977653349No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT12600.10067138224204757No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT12590.10059148431963323No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG12530.10011209678514729No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAA801.2524833E-720.0199491
CATCGTT3700.018.16066428
GACCGGT450.00885490317.7763127
TACCGTG450.00885490317.7763127
ATCGTTT3900.017.6395729
TAGAGCG550.001366222417.4517145
ACCGGTC652.1019136E-417.229358
CTATTCC4550.016.877734
CGTCGTA2950.016.81351710
TACCGTC2950.016.8121747
GATATAC1051.1758675E-716.7786241
GTCCTAT4700.016.6974891
ACCGTCG3000.016.5319718
ATACCGT3000.016.5313116
TCGTTCT4550.016.5261130
CGTTTAT3900.016.40890331
CCTATTC4600.016.349093
CGGCATC4150.016.19208325
GTATTAT805.5737655E-516.0159591
AACGGAC703.6967968E-415.9999615
Produced by FastQC (version 0.11.5)