##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063099_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1251597
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.221973207030697	32.0	32.0	32.0	32.0	32.0
2	30.85884673740829	32.0	32.0	32.0	32.0	32.0
3	30.909027426559827	32.0	32.0	32.0	32.0	32.0
4	30.969117855028415	32.0	32.0	32.0	32.0	32.0
5	30.90328915777203	32.0	32.0	32.0	32.0	32.0
6	34.556326836833264	36.0	36.0	36.0	32.0	36.0
7	34.46837120894345	36.0	36.0	36.0	32.0	36.0
8	34.461046167416505	36.0	36.0	36.0	32.0	36.0
9	34.57627575010167	36.0	36.0	36.0	32.0	36.0
10	34.31342916290148	36.0	36.0	36.0	32.0	36.0
11	34.57487194360485	36.0	36.0	36.0	32.0	36.0
12	34.394154028812785	36.0	36.0	36.0	32.0	36.0
13	34.47689392032739	36.0	36.0	36.0	32.0	36.0
14	34.38617462330127	36.0	36.0	36.0	32.0	36.0
15	34.33976032221234	36.0	36.0	36.0	32.0	36.0
16	34.346593991516436	36.0	36.0	36.0	32.0	36.0
17	34.276114436196316	36.0	36.0	36.0	32.0	36.0
18	34.29965156516035	36.0	36.0	36.0	32.0	36.0
19	34.283694352095765	36.0	36.0	36.0	32.0	36.0
20	34.25970899578698	36.0	36.0	36.0	32.0	36.0
21	34.24052150971918	36.0	36.0	36.0	32.0	36.0
22	34.22210264166501	36.0	36.0	36.0	32.0	36.0
23	34.18126601453982	36.0	36.0	36.0	32.0	36.0
24	34.157813577373545	36.0	36.0	36.0	32.0	36.0
25	34.14008982124438	36.0	36.0	36.0	32.0	36.0
26	34.095816784476156	36.0	36.0	36.0	32.0	36.0
27	34.09550757951641	36.0	36.0	36.0	32.0	36.0
28	34.08034215486295	36.0	36.0	36.0	32.0	36.0
29	34.05474525745907	36.0	36.0	36.0	32.0	36.0
30	34.016544462794336	36.0	36.0	36.0	32.0	36.0
31	34.03582303249369	36.0	36.0	36.0	32.0	36.0
32	33.98423933582455	36.0	36.0	36.0	32.0	36.0
33	33.93442218222	36.0	36.0	36.0	32.0	36.0
34	33.9424375417966	36.0	36.0	36.0	32.0	36.0
35	33.891286092887725	36.0	36.0	36.0	32.0	36.0
36	33.85428376705921	36.0	36.0	36.0	32.0	36.0
37	33.85583458573327	36.0	36.0	36.0	32.0	36.0
38	33.25057027142123	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	5.0
9	3.0
10	10.0
11	15.0
12	8.0
13	5.0
14	208.0
15	398.0
16	571.0
17	647.0
18	777.0
19	997.0
20	1294.0
21	1907.0
22	2719.0
23	3876.0
24	5756.0
25	8397.0
26	12123.0
27	17267.0
28	23947.0
29	32171.0
30	43552.0
31	57593.0
32	78683.0
33	118316.0
34	264514.0
35	575834.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.27978586987563	17.47774744196576	11.494535154122012	25.747931534036596
2	16.744731147208107	20.222177987015634	37.22712406936187	25.805966796414392
3	18.29363110206554	24.84048389017141	28.825649790921027	28.040235216842024
4	12.503735356205164	15.509198963848144	36.28233380261659	35.704731877330104
5	14.662993481107675	36.45833749469676	33.33839355125828	15.54027547293729
6	33.43842606981662	35.911354569736325	17.04475816020064	13.605461200246404
7	29.604017906722373	30.871678343748027	21.48806684579781	18.03623690373179
8	28.24039821347246	32.01463738125105	20.01510079179284	19.729863613483648
9	28.005407504350455	13.955883457415105	18.86090856939231	39.177800468842136
10	16.276336499972036	26.10367612397031	31.116020422023187	26.503966954034468
11	37.304535347232154	21.258436410617392	22.419059596394604	19.017968645755847
12	25.016579335516216	23.978273479934607	28.326214206612875	22.6789329779363
13	29.336543486494822	19.287608094955754	25.42938328941081	25.946465129138613
14	24.144742204117637	19.894941565840508	25.071874096080364	30.88844213396149
15	25.231492395733525	26.56806280002909	22.828302847274557	25.372141956962835
16	25.90343106036937	25.563193629297814	23.57178985823973	24.961585452093082
17	24.017312308808627	25.522949357660917	25.317413638237955	25.142324695292494
18	25.26529676242466	24.244380646620158	26.31697539717608	24.173347193779097
19	25.738788725281818	24.820296793947783	25.6427445929231	23.7981698878473
20	25.926230739482424	23.669462191695885	25.094924268332047	25.309382800489644
21	27.55575833168866	23.868553123928802	24.368186768537704	24.207501775844833
22	26.157104994218972	23.887361397099657	24.9028571154616	25.05267649321977
23	24.349414457030353	23.624437469636145	25.76996343552635	26.256184637807156
24	24.814855064357243	24.97123341526483	25.275040512940166	24.93887100743776
25	24.98483726126178	24.02768688125438	25.430010476074038	25.557465381409806
26	24.701930975844423	25.161260935856827	25.863361323586293	24.273446764712464
27	25.489555275179328	24.546281920873707	24.700577630078634	25.26358517386833
28	24.534666961773773	24.299593375431375	25.99920257158678	25.166537091208074
29	24.45304409527341	24.72664190649092	25.85587212498562	24.96444187325006
30	24.39615070544299	24.917662362151376	26.070069689362185	24.616117243043448
31	25.116338532344667	24.698526591664535	24.61558744566607	25.569547430324725
32	24.711708717135753	24.650422848522428	24.618461508958763	26.019406925383056
33	24.247441264341823	24.225627388639364	25.427627872859464	26.099303474159345
34	25.277178694457387	24.330915602633425	25.546066999134602	24.845838703774582
35	26.12947871934078	24.16818361250997	25.417486403423954	24.284851264725297
36	24.579185115398968	25.17322031162476	24.95682976664501	25.29076480633126
37	25.738583744245823	24.95127780401332	24.854432701043482	24.455705750697373
38	24.70294288497172	24.713889512571445	25.459938139568706	25.123229462888137
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	618.0
1	483.5
2	349.0
3	349.0
4	824.0
5	1299.0
6	1299.0
7	1494.5
8	1690.0
9	1610.0
10	1530.0
11	1530.0
12	1943.5
13	2357.0
14	2963.0
15	3569.0
16	3569.0
17	5388.5
18	7208.0
19	7208.0
20	8434.0
21	9660.0
22	9893.0
23	10126.0
24	10126.0
25	11269.5
26	12413.0
27	12413.0
28	15555.0
29	18697.0
30	22686.0
31	26675.0
32	26675.0
33	34341.0
34	42007.0
35	42007.0
36	46561.0
37	51115.0
38	58769.5
39	66424.0
40	66424.0
41	70900.5
42	75377.0
43	86241.5
44	97106.0
45	97106.0
46	99130.0
47	101154.0
48	101154.0
49	107884.5
50	114615.0
51	115460.5
52	116306.0
53	116306.0
54	110825.0
55	105344.0
56	105344.0
57	103540.0
58	101736.0
59	90903.0
60	80070.0
61	80070.0
62	77133.5
63	74197.0
64	60908.0
65	47619.0
66	47619.0
67	39659.0
68	31699.0
69	31699.0
70	25491.5
71	19284.0
72	15084.5
73	10885.0
74	10885.0
75	8149.5
76	5414.0
77	5414.0
78	5424.5
79	5435.0
80	4228.5
81	3022.0
82	3022.0
83	3154.5
84	3287.0
85	3287.0
86	2175.5
87	1064.0
88	912.0
89	760.0
90	760.0
91	568.5
92	377.0
93	328.5
94	280.0
95	280.0
96	231.5
97	183.0
98	183.0
99	414.5
100	646.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12112525038011437
2	0.05656772906934101
3	0.012464075896634461
4	0.0034356106638159085
5	3.195916896572938E-4
6	3.195916896572938E-4
7	0.0
8	5.592854569002642E-4
9	2.3969376724297038E-4
10	5.592854569002642E-4
11	0.002237141827601057
12	0.0031160189741586152
13	0.012064586284562842
14	0.008229486008675317
15	0.020773459827724102
16	0.009427954844890169
17	0.018616215922537364
18	0.005912446258659936
19	0.007110915094874788
20	0.005832548336245613
21	0.006391833793145877
22	0.007190813017289112
23	0.008708873543161258
24	0.011585198750076901
25	0.013902238500092281
26	0.016698665784593603
27	0.00830938393108964
28	0.005912446258659936
29	0.009987240301790433
30	0.0034356106638159085
31	0.006152140025902907
32	0.006791323405217493
33	0.007989792241432345
34	0.011824892517319873
35	0.0143017281121639
36	0.012464075896634461
37	0.009108363155232875
38	0.005592854569002642
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1251597.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.85890929648298
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.50587951786757	41.230881944541984
2	12.424127834733863	12.886034369387458
3	3.6370858262840944	5.658459119063721
4	1.5092838121667274	3.1307924927121746
5	0.7871603650011442	2.041063898519039
6	0.45179736138382537	1.4057831030636485
7	0.31149911698428207	1.1307803117535697
8	0.208413223787843	0.8646465974881088
9	0.15440938065335524	0.7206751859245618
>10	0.8060531765652865	7.553899906084341
>50	0.08653741766854825	3.1715592925047877
>100	0.09779221888188759	10.883844110107239
>500	0.014390306713313848	4.958333155293893
>1k	0.005570441308379554	4.363246513555459
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4536	0.3624169760713712	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2996	0.23937417555331308	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2011	0.1606747219752045	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1810	0.14461523956992547	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1782	0.1423780977423244	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1765	0.1410198330612809	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1645	0.1314320823715621	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1627	0.12999391976810426	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1622	0.12959443015603264	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1621	0.12951453223361833	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1579	0.12615881949221674	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1552	0.12400157558703001	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1539	0.12296290259564381	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1527	0.12200412752667192	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1526	0.1219242296042576	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1506	0.12032627115597114	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1451	0.11593188542318333	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1352	0.10802199110416533	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	1293	0.10330801368172024	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1288	0.10290852406964861	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1266	0.10115076977653349	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	1260	0.10067138224204757	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1259	0.10059148431963323	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1253	0.10011209678514729	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	7.989792241432346E-5	0.0	0.0	0.0
4	0.0	7.989792241432346E-5	0.0	0.0	0.0
5	0.0	7.989792241432346E-5	0.0	0.0	0.0
6	0.0	7.989792241432346E-5	0.0	0.0	0.0
7	0.0	7.989792241432346E-5	0.0	0.0	0.0
8	0.0	7.989792241432346E-5	0.0	0.0	0.0
9	0.0	7.989792241432346E-5	0.0	1.597958448286469E-4	0.0
10	0.0	7.989792241432346E-5	0.0	1.597958448286469E-4	0.0
11	0.0	7.989792241432346E-5	0.0	1.597958448286469E-4	0.0
12	0.0	7.989792241432346E-5	0.0	1.597958448286469E-4	7.989792241432346E-5
13	0.0	7.989792241432346E-5	0.0	1.597958448286469E-4	3.195916896572938E-4
14	0.0	7.989792241432346E-5	0.0	1.597958448286469E-4	3.195916896572938E-4
15	0.0	7.989792241432346E-5	0.0	2.3969376724297038E-4	4.7938753448594076E-4
16	0.0	7.989792241432346E-5	0.0	3.195916896572938E-4	4.7938753448594076E-4
17	0.0	7.989792241432346E-5	0.0	3.195916896572938E-4	4.7938753448594076E-4
18	7.989792241432346E-5	7.989792241432346E-5	0.0	3.994896120716173E-4	4.7938753448594076E-4
19	7.989792241432346E-5	7.989792241432346E-5	0.0	5.592854569002642E-4	4.7938753448594076E-4
20	7.989792241432346E-5	7.989792241432346E-5	0.0	7.190813017289112E-4	6.391833793145876E-4
21	7.989792241432346E-5	7.989792241432346E-5	0.0	8.788771465575581E-4	7.989792241432346E-4
22	7.989792241432346E-5	7.989792241432346E-5	0.0	0.0017577542931151162	7.989792241432346E-4
23	1.597958448286469E-4	7.989792241432346E-5	0.0	0.0019974480603580863	9.587750689718815E-4
24	1.597958448286469E-4	7.989792241432346E-5	0.0	0.0030361210517442917	0.001038672991386205
25	1.597958448286469E-4	7.989792241432346E-5	0.0	0.003435610663815909	0.001038672991386205
26	1.597958448286469E-4	7.989792241432346E-5	0.0	0.004314487810373467	0.0011185709138005285
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCTAA	80	1.2524833E-7	20.019949	1
CATCGTT	370	0.0	18.160664	28
GACCGGT	45	0.008854903	17.776312	7
TACCGTG	45	0.008854903	17.776312	7
ATCGTTT	390	0.0	17.63957	29
TAGAGCG	55	0.0013662224	17.451714	5
ACCGGTC	65	2.1019136E-4	17.22935	8
CTATTCC	455	0.0	16.87773	4
CGTCGTA	295	0.0	16.813517	10
TACCGTC	295	0.0	16.812174	7
GATATAC	105	1.1758675E-7	16.778624	1
GTCCTAT	470	0.0	16.697489	1
ACCGTCG	300	0.0	16.531971	8
ATACCGT	300	0.0	16.531311	6
TCGTTCT	455	0.0	16.52611	30
CGTTTAT	390	0.0	16.408903	31
CCTATTC	460	0.0	16.34909	3
CGGCATC	415	0.0	16.192083	25
GTATTAT	80	5.5737655E-5	16.015959	1
AACGGAC	70	3.6967968E-4	15.99996	15
>>END_MODULE