FastQCFastQC Report
Thu 2 Feb 2017
SRR4063099_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063099_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1251597
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT49530.39573440971814405No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT34660.2769261990880451No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG21800.17417747086322513No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT19360.15468237779413022No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC18330.1464528917854549No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA18220.14557401463889735No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA17740.14173891436300984No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA17330.13846309954402256No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG17200.13742442655263634No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC17190.13734452863022203No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC16940.13534708056986394No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT15500.12384177974220137No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG15460.12352218805254406No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG15450.12344229013012975No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG15410.12312269844047247No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG15220.12160463791460031No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15040.12016647531114248No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT14980.11968708777665654No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC14310.11433392697489687No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG13980.11169729553522419No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT13830.11049882669900936No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT13580.10850137863865127No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT13490.10778229733692235No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC13340.1065838285007075No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT13190.10538535966449264No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT13140.10498587005242103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG2450.020.2492477
ACTATCC507.1860343E-419.2041248
TACCGTC3350.018.1531527
GAACCGC2800.017.718096
ACCGTCG3350.017.6754388
ACTATAC550.001364235317.4555033
GTATTAT1051.18856406E-716.7628251
ATACCGT3650.016.6611126
CGTCGTA3800.016.42261110
GTCTAAC908.577548E-616.0008771
CCGTCGT3750.015.78879459
TATAAGA1753.6379788E-1215.5430892
TAGAACC3400.015.0608554
TCTAGAT3200.015.0002252
TTTCGTT4300.014.88275428
TCTAGCG3350.014.80500928
GTCGTAG4000.014.80022111
TAAACGC4000.014.79903928
GGATAGT650.00415591514.7724046
CGTCTTA1308.317511E-814.76827315
Produced by FastQC (version 0.11.5)