##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063099_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1251597
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.191557665926013	32.0	32.0	32.0	32.0	32.0
2	31.307148387220487	32.0	32.0	32.0	32.0	32.0
3	31.386642026147396	32.0	32.0	32.0	32.0	32.0
4	31.489501013505144	32.0	32.0	32.0	32.0	32.0
5	31.41574404540759	32.0	32.0	32.0	32.0	32.0
6	34.93983926135969	36.0	36.0	36.0	36.0	36.0
7	34.96527796087718	36.0	36.0	36.0	36.0	36.0
8	34.91560062863685	36.0	36.0	36.0	36.0	36.0
9	35.00974355163843	36.0	36.0	36.0	36.0	36.0
10	34.85526171762956	36.0	36.0	36.0	32.0	36.0
11	35.03707742987559	36.0	36.0	36.0	36.0	36.0
12	34.93105847968635	36.0	36.0	36.0	32.0	36.0
13	34.98608977170767	36.0	36.0	36.0	36.0	36.0
14	34.93496788503008	36.0	36.0	36.0	32.0	36.0
15	34.89758764202855	36.0	36.0	36.0	32.0	36.0
16	34.908645514490686	36.0	36.0	36.0	32.0	36.0
17	34.870120334260946	36.0	36.0	36.0	32.0	36.0
18	34.87010675161414	36.0	36.0	36.0	32.0	36.0
19	34.86693720103196	36.0	36.0	36.0	32.0	36.0
20	34.85239098527721	36.0	36.0	36.0	32.0	36.0
21	34.840312017366614	36.0	36.0	36.0	32.0	36.0
22	34.81714401680413	36.0	36.0	36.0	32.0	36.0
23	34.77119072672753	36.0	36.0	36.0	32.0	36.0
24	34.74720776735643	36.0	36.0	36.0	32.0	36.0
25	34.72342055789523	36.0	36.0	36.0	32.0	36.0
26	34.66201101472758	36.0	36.0	36.0	32.0	36.0
27	34.646951055331705	36.0	36.0	36.0	32.0	36.0
28	34.61684711612444	36.0	36.0	36.0	32.0	36.0
29	34.57387242059545	36.0	36.0	36.0	32.0	36.0
30	34.553187647461606	36.0	36.0	36.0	32.0	36.0
31	34.553970647101266	36.0	36.0	36.0	32.0	36.0
32	34.5199453178619	36.0	36.0	36.0	32.0	36.0
33	34.4753718649054	36.0	36.0	36.0	32.0	36.0
34	34.46967753997493	36.0	36.0	36.0	32.0	36.0
35	34.42070011353495	36.0	36.0	36.0	32.0	36.0
36	34.39308579359011	36.0	36.0	36.0	32.0	36.0
37	34.389748457370864	36.0	36.0	36.0	32.0	36.0
38	33.95249429329089	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	10.0
21	25.0
22	95.0
23	324.0
24	806.0
25	2096.0
26	4372.0
27	8288.0
28	14369.0
29	22974.0
30	34476.0
31	49904.0
32	70905.0
33	114894.0
34	280894.0
35	647165.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.79445207088488	18.653225664685568	12.15818853306528	26.39413373136427
2	15.769145954445365	21.249988015302062	36.53583105091419	26.445034979338384
3	17.447149521770985	25.568613539342138	29.10785180852942	27.876385130357455
4	11.984637190654478	16.337885927864786	36.40182039862767	35.275656482853066
5	14.088332017679829	37.01026529327355	33.16221847944544	15.73918420960118
6	33.45371306213272	36.36256137168972	16.67656070853591	13.507164857641648
7	29.286803756179136	31.044326780873305	21.575338908637086	18.093530554310476
8	28.054555696268274	32.406786703047786	19.934242328604586	19.604415272079358
9	27.84868990347162	13.779404561866935	18.721520292054088	39.65038524260736
10	16.021125213229624	26.477706268451602	31.14666602747716	26.354502490841615
11	37.317963395321264	21.172177503767376	22.496072819435987	19.013786281475372
12	24.806326662362814	24.08923994400731	28.405422853453842	22.699010540176033
13	29.38274761404448	19.256868236130696	25.469660712930303	25.890723436894525
14	24.001255995340355	19.829385976476456	25.098494163856255	31.070863864326938
15	25.205157890279377	26.766762783867332	22.63076693216746	25.397312393685826
16	25.840627359489293	25.528146085594784	23.529656158741446	25.101570396174484
17	23.977686108228127	25.623982799575263	25.166647091675674	25.231684000520932
18	25.35928098261661	24.11247390334109	26.31398125754536	24.214263856496938
19	25.771873853964177	24.7936036919232	25.620547188911445	23.81397526520118
20	25.964827336594766	23.660491356243263	25.145953529770367	25.2287277773916
21	27.46122145040644	23.949459649055523	24.384584777491742	24.204734123046293
22	26.036415875078	23.85999646851183	24.99582533355385	25.10776232285632
23	24.255650980307557	23.592098734656602	25.843861882059482	26.308388402976355
24	24.866550495087473	24.90913608773431	25.25557347932282	24.968739937855396
25	24.94075554852452	23.991645853207988	25.56196338429235	25.505635213975136
26	24.748640935253867	24.965963457857008	25.971479614827786	24.313915992061336
27	25.65853970333854	24.3596371030565	24.8097028192027	25.17212037440226
28	24.39059857126535	24.264679445540377	26.12550205856997	25.219219924624298
29	24.527224018593845	24.510365556964423	25.93662336998251	25.025787054459226
30	24.477767204619376	24.61543132493926	26.190618865337644	24.71618260510372
31	25.140999858580678	24.5565465561199	24.701721081146726	25.600732504152695
32	24.81821225202681	24.525546162223144	24.714664544577847	25.9415770411722
33	24.39467336530848	24.009805073038688	25.588907611635374	26.00661395001746
34	25.281859895797133	24.13644328006539	25.76100773651583	24.820689087621655
35	26.244629860889727	24.04144465031476	25.483042864436396	24.23088262435912
36	24.844179076811464	24.916806288286086	24.959072289243263	25.279942345659183
37	25.84721759480088	24.790967060483528	24.918164552967127	24.44365079174846
38	24.661751334697993	24.356940030073652	25.66559123805324	25.315717397175124
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	313.0
1	255.5
2	198.0
3	198.0
4	685.0
5	1172.0
6	1172.0
7	1428.0
8	1684.0
9	1594.0
10	1504.0
11	1504.0
12	1957.5
13	2411.0
14	3071.5
15	3732.0
16	3732.0
17	5562.0
18	7392.0
19	7392.0
20	8584.5
21	9777.0
22	9983.5
23	10190.0
24	10190.0
25	11287.0
26	12384.0
27	12384.0
28	15638.0
29	18892.0
30	22680.0
31	26468.0
32	26468.0
33	34725.5
34	42983.0
35	42983.0
36	46883.0
37	50783.0
38	58771.5
39	66760.0
40	66760.0
41	71378.0
42	75996.0
43	87122.5
44	98249.0
45	98249.0
46	99627.5
47	101006.0
48	101006.0
49	108082.0
50	115158.0
51	115570.5
52	115983.0
53	115983.0
54	110875.5
55	105768.0
56	105768.0
57	103806.0
58	101844.0
59	91023.0
60	80202.0
61	80202.0
62	76995.5
63	73789.0
64	60780.0
65	47771.0
66	47771.0
67	39724.5
68	31678.0
69	31678.0
70	25484.5
71	19291.0
72	14915.5
73	10540.0
74	10540.0
75	7883.5
76	5227.0
77	5227.0
78	5237.0
79	5247.0
80	4119.5
81	2992.0
82	2992.0
83	2882.0
84	2772.0
85	2772.0
86	1762.0
87	752.0
88	632.5
89	513.0
90	513.0
91	304.5
92	96.0
93	71.0
94	46.0
95	46.0
96	31.0
97	16.0
98	16.0
99	17.0
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008229486008675317
2	7.989792241432346E-5
3	0.0
4	2.3969376724297038E-4
5	7.989792241432346E-5
6	1.597958448286469E-4
7	4.7938753448594076E-4
8	0.0025567335172583506
9	0.003835100275887526
10	9.587750689718815E-4
11	0.005033569112102378
12	3.994896120716173E-4
13	1.597958448286469E-4
14	0.0
15	0.0
16	1.597958448286469E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	2.3969376724297038E-4
22	0.0
23	0.0
24	0.0
25	2.3969376724297038E-4
26	7.989792241432346E-5
27	1.597958448286469E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	2.3969376724297038E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1251597.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.706337016396525
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.85764085608281	38.700181356940156
2	13.303422450590116	13.22528799601056
3	3.979750495737157	5.934564581468468
4	1.6757549147334418	3.3318255419449314
5	0.8352101564406673	2.0757618757778533
6	0.5279797820952908	1.5746364592003275
7	0.33549929825819447	1.1673508831590445
8	0.23728281863562087	0.9435567801042131
9	0.17495783529148165	0.7826861812191822
>10	0.8518080862237389	7.663310880270535
>50	0.09356639962206714	3.2754159924000166
>100	0.10564294722618298	11.322754961269768
>500	0.01566875209881419	5.40375876001643
>1k	0.005815206964508359	4.5989077502185856
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4953	0.39573440971814405	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3466	0.2769261990880451	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2180	0.17417747086322513	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1936	0.15468237779413022	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1833	0.1464528917854549	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1822	0.14557401463889735	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1774	0.14173891436300984	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1733	0.13846309954402256	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1720	0.13742442655263634	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1719	0.13734452863022203	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1694	0.13534708056986394	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1550	0.12384177974220137	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1546	0.12352218805254406	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1545	0.12344229013012975	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1541	0.12312269844047247	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1522	0.12160463791460031	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1504	0.12016647531114248	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1498	0.11968708777665654	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1431	0.11433392697489687	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1398	0.11169729553522419	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1383	0.11049882669900936	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	1358	0.10850137863865127	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1349	0.10778229733692235	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	1334	0.1065838285007075	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1319	0.10538535966449264	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1314	0.10498587005242103	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.989792241432346E-5
2	0.0	0.0	0.0	0.0	7.989792241432346E-5
3	0.0	0.0	0.0	0.0	7.989792241432346E-5
4	0.0	0.0	0.0	0.0	7.989792241432346E-5
5	0.0	0.0	0.0	0.0	1.597958448286469E-4
6	0.0	0.0	0.0	0.0	1.597958448286469E-4
7	0.0	0.0	0.0	0.0	1.597958448286469E-4
8	0.0	0.0	0.0	0.0	1.597958448286469E-4
9	0.0	0.0	0.0	7.989792241432346E-5	1.597958448286469E-4
10	0.0	0.0	0.0	7.989792241432346E-5	2.3969376724297038E-4
11	0.0	0.0	0.0	7.989792241432346E-5	2.3969376724297038E-4
12	0.0	0.0	0.0	7.989792241432346E-5	7.190813017289112E-4
13	0.0	0.0	0.0	7.989792241432346E-5	8.788771465575581E-4
14	0.0	0.0	0.0	7.989792241432346E-5	8.788771465575581E-4
15	0.0	0.0	0.0	1.597958448286469E-4	0.0011185709138005285
16	0.0	0.0	0.0	2.3969376724297038E-4	0.0011185709138005285
17	0.0	0.0	0.0	2.3969376724297038E-4	0.0011185709138005285
18	0.0	0.0	0.0	3.195916896572938E-4	0.0011185709138005285
19	0.0	0.0	0.0	4.7938753448594076E-4	0.0011185709138005285
20	0.0	0.0	0.0	7.190813017289112E-4	0.001198468836214852
21	0.0	0.0	0.0	8.788771465575581E-4	0.001198468836214852
22	0.0	0.0	0.0	0.0016778563707007927	0.0013582646810434988
23	0.0	0.0	0.0	0.001917550137943763	0.0013582646810434988
24	0.0	0.0	0.0	0.0028763252069156447	0.0013582646810434988
25	0.0	0.0	0.0	0.003275814818987262	0.0013582646810434988
26	0.0	0.0	0.0	0.00415469196554482	0.0013582646810434988
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGCG	245	0.0	20.249247	7
ACTATCC	50	7.1860343E-4	19.204124	8
TACCGTC	335	0.0	18.153152	7
GAACCGC	280	0.0	17.71809	6
ACCGTCG	335	0.0	17.675438	8
ACTATAC	55	0.0013642353	17.455503	3
GTATTAT	105	1.18856406E-7	16.762825	1
ATACCGT	365	0.0	16.661112	6
CGTCGTA	380	0.0	16.422611	10
GTCTAAC	90	8.577548E-6	16.000877	1
CCGTCGT	375	0.0	15.7887945	9
TATAAGA	175	3.6379788E-12	15.543089	2
TAGAACC	340	0.0	15.060855	4
TCTAGAT	320	0.0	15.000225	2
TTTCGTT	430	0.0	14.882754	28
TCTAGCG	335	0.0	14.805009	28
GTCGTAG	400	0.0	14.800221	11
TAAACGC	400	0.0	14.799039	28
GGATAGT	65	0.004155915	14.772404	6
CGTCTTA	130	8.317511E-8	14.768273	15
>>END_MODULE