FastQCFastQC Report
Thu 2 Feb 2017
SRR4063098_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063098_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693916
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT28460.41013609716449834No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT19910.286922336421123No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT11170.16097049210567274No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8300.11961101920117131No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT8110.11687293562909631No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG7800.1124055361167634No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA7520.108370465589495No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG7400.10664114964923709No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA7290.10505594337066734No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG7240.10433539506222655No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC7220.10404717573885025No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC7130.1027501887836568No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGTC502.8008497E-522.3945033
CTAGCGG1400.021.71208629
TCTAGCG1400.021.7105228
TAGCGGC1500.020.26607330
CCCCTAG400.00445078420.0239491
CCTACTA507.200402E-419.19667226
ATCGTTT1401.8189894E-1218.28386129
GTTCAAA2850.017.9864231
GCTTGTA450.00882997517.7836672
CATACGT450.00884465517.7785419
ACATACG450.00884832917.77725818
ACCGTCC450.00885567917.7746968
TGGATAC2350.017.70288524
CCGAGCC2550.017.5693814
CATCGTT1550.017.54534328
ACGTTTT1201.6570993E-917.33157729
CGGTCCA1850.017.29803710
TCCGAAA1950.017.23150621
CGAGCCG2350.017.02200515
ACCGCAG2550.016.93945929
Produced by FastQC (version 0.11.5)