##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063098_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	693916
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.184715441062032	32.0	32.0	32.0	32.0	32.0
2	30.85266084079341	32.0	32.0	32.0	32.0	32.0
3	30.9051081110682	32.0	32.0	32.0	32.0	32.0
4	30.95077358066394	32.0	32.0	32.0	32.0	32.0
5	30.915337014854824	32.0	32.0	32.0	32.0	32.0
6	34.55182327543968	36.0	36.0	36.0	32.0	36.0
7	34.455641605035765	36.0	36.0	36.0	32.0	36.0
8	34.433595708990715	36.0	36.0	36.0	32.0	36.0
9	34.5395494555537	36.0	36.0	36.0	32.0	36.0
10	34.305743058237596	36.0	36.0	36.0	32.0	36.0
11	34.55454550694897	36.0	36.0	36.0	32.0	36.0
12	34.388294836839044	36.0	36.0	36.0	32.0	36.0
13	34.45809291038108	36.0	36.0	36.0	32.0	36.0
14	34.37991774220511	36.0	36.0	36.0	32.0	36.0
15	34.332672830717264	36.0	36.0	36.0	32.0	36.0
16	34.33881622559503	36.0	36.0	36.0	32.0	36.0
17	34.267794661025256	36.0	36.0	36.0	32.0	36.0
18	34.287988747917616	36.0	36.0	36.0	32.0	36.0
19	34.273580663942035	36.0	36.0	36.0	32.0	36.0
20	34.24777062353368	36.0	36.0	36.0	32.0	36.0
21	34.22473901740268	36.0	36.0	36.0	32.0	36.0
22	34.21387891329786	36.0	36.0	36.0	32.0	36.0
23	34.152554487863085	36.0	36.0	36.0	32.0	36.0
24	34.14325797358758	36.0	36.0	36.0	32.0	36.0
25	34.11824918289822	36.0	36.0	36.0	32.0	36.0
26	34.08083975582059	36.0	36.0	36.0	32.0	36.0
27	34.06754131624	36.0	36.0	36.0	32.0	36.0
28	34.07306503957251	36.0	36.0	36.0	32.0	36.0
29	34.021957989151424	36.0	36.0	36.0	32.0	36.0
30	33.987015719481896	36.0	36.0	36.0	32.0	36.0
31	34.010622323163034	36.0	36.0	36.0	32.0	36.0
32	33.95908150266026	36.0	36.0	36.0	32.0	36.0
33	33.92491886626047	36.0	36.0	36.0	32.0	36.0
34	33.91608494399899	36.0	36.0	36.0	32.0	36.0
35	33.86311167345904	36.0	36.0	36.0	32.0	36.0
36	33.830097591062895	36.0	36.0	36.0	32.0	36.0
37	33.810889214256484	36.0	36.0	36.0	32.0	36.0
38	33.21333129658345	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	1.0
8	1.0
9	3.0
10	3.0
11	11.0
12	3.0
13	6.0
14	177.0
15	294.0
16	405.0
17	458.0
18	486.0
19	646.0
20	791.0
21	1051.0
22	1571.0
23	2277.0
24	3136.0
25	4658.0
26	6616.0
27	9608.0
28	13455.0
29	17939.0
30	24133.0
31	32153.0
32	43428.0
33	65458.0
34	146623.0
35	318523.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.63696702979583	17.421253877786597	11.462520741649232	25.47925835076834
2	16.089653561427745	20.4527954734872	38.427054104170935	25.030496860914127
3	19.189531593883725	24.64180242454466	28.945938505752927	27.222727475818694
4	12.272211742020836	15.78971640046635	36.79645943729725	35.141612420215566
5	13.990206279164278	37.22219179898374	33.9438316563724	14.843770265479584
6	33.02205601550622	36.38039241117428	17.381667783518154	13.215883789801344
7	29.01349588926598	31.18768148836673	21.248712018042557	18.550110604324736
8	27.57218844437271	33.45664370029096	20.047469927786334	18.923697927549995
9	27.54451926970672	14.110702638515201	18.896605194022882	39.4481728977552
10	15.60325285734667	27.29636135942114	32.1175709346849	24.982814848547296
11	37.144347680721026	21.506814372655462	22.500327856567434	18.848510090056074
12	24.344028995741432	24.311459226540038	29.604046721766263	21.740465055952267
13	29.737738753437533	19.8356327074193	25.4933613140159	24.933267225127267
14	23.27898433232591	20.355725813541564	25.91716641517102	30.448123438961506
15	24.863891695699742	27.624111890611736	23.307709272373724	24.204287141314797
16	24.89050053398555	26.120468869579256	25.06618970683401	23.92284088960119
17	23.24029030002955	26.10235454368554	26.12411983885033	24.533235317434578
18	24.039845908401105	25.31140424832786	27.533568823113164	23.11518102015787
19	24.948656567563635	25.32784132753579	26.226009105622627	23.49749299927795
20	25.073644706804714	24.734965526783036	26.34578135448613	23.84560841192612
21	26.59087830144899	24.521878990346785	25.092741784389403	23.79450092381482
22	24.98014749224275	25.032318873789933	25.946038650058295	24.041494983909022
23	23.883513895634653	24.847121356026005	26.138176640229677	25.131188108109665
24	24.07581968394532	25.846780935249726	26.14873941343665	23.928659967368308
25	24.488207475781472	24.92724712339666	26.211358817619818	24.37318658320205
26	23.332300352414652	26.180589087397394	26.94278486851114	23.544325691676814
27	24.52209103906286	25.315195083706126	26.211922935018215	23.950790942212794
28	23.536931429222026	25.72351761937086	26.572959947655672	24.16659100375144
29	23.569436537505315	25.429821211094854	26.638610044174765	24.36213220722506
30	23.619814034460695	26.07423057190456	26.662362442570313	23.64359295106443
31	24.151030955321666	25.698004384100216	25.705930929524364	24.445033731053755
32	23.747229276138484	25.872003366663687	25.6735489189493	24.70721843824853
33	23.577472461746222	25.252178775619623	26.41034916617785	24.759999596456307
34	24.14622581003155	25.84491036001562	26.54032466119271	23.468539168760117
35	24.794213919513176	25.4396420886044	26.66362065312488	23.102523338757543
36	23.45508188876734	26.249663090744257	26.029861330312322	24.265393690176086
37	24.421089952107597	26.078659992764976	25.888416322330425	23.611833732797
38	23.667799922463974	26.047781215816773	26.016939748166447	24.267479113552806
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	557.0
1	412.0
2	267.0
3	267.0
4	559.5
5	852.0
6	852.0
7	982.0
8	1112.0
9	1041.5
10	971.0
11	971.0
12	1260.5
13	1550.0
14	1945.5
15	2341.0
16	2341.0
17	3498.0
18	4655.0
19	4655.0
20	5457.5
21	6260.0
22	6560.5
23	6861.0
24	6861.0
25	8220.0
26	9579.0
27	9579.0
28	12395.0
29	15211.0
30	18136.0
31	21061.0
32	21061.0
33	25171.5
34	29282.0
35	29282.0
36	31142.0
37	33002.0
38	37182.5
39	41363.0
40	41363.0
41	43222.0
42	45081.0
43	49720.0
44	54359.0
45	54359.0
46	55668.5
47	56978.0
48	56978.0
49	59162.5
50	61347.0
51	60900.0
52	60453.0
53	60453.0
54	57209.5
55	53966.0
56	53966.0
57	52153.0
58	50340.0
59	44712.0
60	39084.0
61	39084.0
62	37056.5
63	35029.0
64	28986.5
65	22944.0
66	22944.0
67	19182.5
68	15421.0
69	15421.0
70	12297.5
71	9174.0
72	7162.5
73	5151.0
74	5151.0
75	3899.0
76	2647.0
77	2647.0
78	2589.5
79	2532.0
80	1972.5
81	1413.0
82	1413.0
83	1444.0
84	1475.0
85	1475.0
86	1005.5
87	536.0
88	463.0
89	390.0
90	390.0
91	271.5
92	153.0
93	136.0
94	119.0
95	119.0
96	104.5
97	90.0
98	90.0
99	200.0
100	310.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12479896702194501
2	0.057932083998639604
3	0.013690417860375032
4	0.0021616449253223733
5	2.8821932337631643E-4
6	1.4410966168815822E-4
7	1.4410966168815822E-4
8	4.323289850644746E-4
9	4.323289850644746E-4
10	4.323289850644746E-4
11	0.0021616449253223733
12	0.0030263028954513227
13	0.014987404815568456
14	0.0096553473331066
15	0.024066313501922423
16	0.011096443949988183
17	0.021760558914911892
18	0.006196715452590804
19	0.008502470039601336
20	0.006340825114278962
21	0.007781921731160544
22	0.0070613734227197526
23	0.009367128009730285
24	0.01614028210907372
25	0.01859014635777241
26	0.018878365681148725
27	0.008646579701289493
28	0.0070613734227197526
29	0.011672882596740815
30	0.0034586318805157968
31	0.00648493477596712
32	0.007781921731160544
33	0.009078908686353969
34	0.01224932124349345
35	0.01628439177076188
36	0.015707953124009248
37	0.009943566656482918
38	0.00648493477596712
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	693916.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.03449657706197
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.33862614522451	51.69869718342978
2	11.346153917496414	14.077058927154992
3	2.6820321371081377	4.991355402870149
4	0.9227428560977453	2.289675541924159
5	0.40270136651101235	1.2490688271202786
6	0.24791970314833747	0.9227744385805058
7	0.14819351117798332	0.643517690333938
8	0.11373453336087974	0.5644371616375388
9	0.08049430653582894	0.4494081404942871
>10	0.5477530387654279	6.732006026642015
>50	0.08716111657971534	3.8463575827888543
>100	0.07571078535437697	9.260143337594025
>500	0.006075556849328258	2.41241222621635
>1k	7.010257903071067E-4	0.8630875132130842
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2846	0.41013609716449834	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1991	0.286922336421123	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1117	0.16097049210567274	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	830	0.11961101920117131	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	811	0.11687293562909631	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	780	0.1124055361167634	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	752	0.108370465589495	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	740	0.10664114964923709	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	729	0.10505594337066734	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	724	0.10433539506222655	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	722	0.10404717573885025	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	713	0.1027501887836568	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	1.4410966168815822E-4
13	0.0	0.0	0.0	0.0	1.4410966168815822E-4
14	0.0	0.0	0.0	0.0	1.4410966168815822E-4
15	0.0	0.0	0.0	0.0	2.8821932337631643E-4
16	0.0	1.4410966168815822E-4	0.0	2.8821932337631643E-4	2.8821932337631643E-4
17	0.0	1.4410966168815822E-4	0.0	2.8821932337631643E-4	2.8821932337631643E-4
18	0.0	1.4410966168815822E-4	0.0	7.205483084407911E-4	4.3232898506447465E-4
19	0.0	1.4410966168815822E-4	0.0	0.0010087676318171076	4.3232898506447465E-4
20	0.0	1.4410966168815822E-4	0.0	0.001296986955193424	4.3232898506447465E-4
21	0.0	1.4410966168815822E-4	0.0	0.0014410966168815823	4.3232898506447465E-4
22	0.0	1.4410966168815822E-4	0.0	0.002017535263634215	4.3232898506447465E-4
23	0.0	1.4410966168815822E-4	0.0	0.003890960865580272	4.3232898506447465E-4
24	0.0	1.4410966168815822E-4	0.0	0.005620276805838171	4.3232898506447465E-4
25	0.0	1.4410966168815822E-4	0.0	0.007205483084407911	4.3232898506447465E-4
26	0.0	1.4410966168815822E-4	0.0	0.008790689362977652	4.3232898506447465E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGGTC	50	2.8008497E-5	22.394503	3
CTAGCGG	140	0.0	21.712086	29
TCTAGCG	140	0.0	21.71052	28
TAGCGGC	150	0.0	20.266073	30
CCCCTAG	40	0.004450784	20.023949	1
CCTACTA	50	7.200402E-4	19.196672	26
ATCGTTT	140	1.8189894E-12	18.283861	29
GTTCAAA	285	0.0	17.986423	1
GCTTGTA	45	0.008829975	17.783667	2
CATACGT	45	0.008844655	17.77854	19
ACATACG	45	0.008848329	17.777258	18
ACCGTCC	45	0.008855679	17.774696	8
TGGATAC	235	0.0	17.702885	24
CCGAGCC	255	0.0	17.56938	14
CATCGTT	155	0.0	17.545343	28
ACGTTTT	120	1.6570993E-9	17.331577	29
CGGTCCA	185	0.0	17.298037	10
TCCGAAA	195	0.0	17.231506	21
CGAGCCG	235	0.0	17.022005	15
ACCGCAG	255	0.0	16.939459	29
>>END_MODULE