FastQCFastQC Report
Thu 2 Feb 2017
SRR4063098_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063098_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693916
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT30430.43852570051706546No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT24100.3473042846684613No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT13630.19642146888095968No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10080.1452625389816635No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT8940.12883403754921346No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC8520.1227814317583108No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG8390.12090800615636474No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC7540.1086586849128713No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA7400.10664114964923709No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC7300.1052000530323555No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC7230.10419128540053839No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA7200.10375895641547392No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG7150.10303840810703313No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGACGC200.003752540631.99992828
GCCGCGC350.00206574722.8570928
AACTTAT604.716205E-621.3332847
AACGGAC400.00448177819.99995415
ACTTATC751.5068399E-619.1999578
TCGAACT507.1921165E-419.19995719
TCGTTTA1950.018.87175230
ATCGTTT1950.018.87175229
CGTTTAT1950.018.87175231
CATCGTT2000.018.39995828
ATAACAC802.7930037E-617.999963
ATTAGCT904.3606633E-717.7777393
ACCGCTG450.008846953517.7777398
TAGATAA1550.017.5483474
ACTTATT550.00136392817.4545068
GGCATCG2200.017.45450626
TCTAGCG1850.017.29725828
CTAGCGG1850.017.29725829
CGCGTAA652.0989291E-417.2307310
ATATAAC753.2307704E-517.066633
Produced by FastQC (version 0.11.5)