##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063098_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	693916
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.151074481637547	32.0	32.0	32.0	32.0	32.0
2	31.29624046714588	32.0	32.0	32.0	32.0	32.0
3	31.378377209921663	32.0	32.0	32.0	32.0	32.0
4	31.478752471480696	32.0	32.0	32.0	32.0	32.0
5	31.407000270926165	32.0	32.0	32.0	32.0	32.0
6	34.92951740556494	36.0	36.0	36.0	36.0	36.0
7	34.954751007326536	36.0	36.0	36.0	36.0	36.0
8	34.902370027496126	36.0	36.0	36.0	36.0	36.0
9	34.988892027277075	36.0	36.0	36.0	36.0	36.0
10	34.85522166948161	36.0	36.0	36.0	32.0	36.0
11	35.01557825442849	36.0	36.0	36.0	36.0	36.0
12	34.93638711313761	36.0	36.0	36.0	32.0	36.0
13	34.97558205892356	36.0	36.0	36.0	36.0	36.0
14	34.92073536278166	36.0	36.0	36.0	32.0	36.0
15	34.886790331971014	36.0	36.0	36.0	32.0	36.0
16	34.897120112520824	36.0	36.0	36.0	32.0	36.0
17	34.86631667233498	36.0	36.0	36.0	32.0	36.0
18	34.86988482755838	36.0	36.0	36.0	32.0	36.0
19	34.85137826480439	36.0	36.0	36.0	32.0	36.0
20	34.833798327175046	36.0	36.0	36.0	32.0	36.0
21	34.81954444053747	36.0	36.0	36.0	32.0	36.0
22	34.80761792493616	36.0	36.0	36.0	32.0	36.0
23	34.74339977749468	36.0	36.0	36.0	32.0	36.0
24	34.72608500164285	36.0	36.0	36.0	32.0	36.0
25	34.69847791375325	36.0	36.0	36.0	32.0	36.0
26	34.63817090252999	36.0	36.0	36.0	32.0	36.0
27	34.63300168896524	36.0	36.0	36.0	32.0	36.0
28	34.597598268378306	36.0	36.0	36.0	32.0	36.0
29	34.55472996731593	36.0	36.0	36.0	32.0	36.0
30	34.53599427019985	36.0	36.0	36.0	32.0	36.0
31	34.5232794747491	36.0	36.0	36.0	32.0	36.0
32	34.492687875765945	36.0	36.0	36.0	32.0	36.0
33	34.44155344450913	36.0	36.0	36.0	32.0	36.0
34	34.43078124729794	36.0	36.0	36.0	32.0	36.0
35	34.39230540872382	36.0	36.0	36.0	32.0	36.0
36	34.363427273618136	36.0	36.0	36.0	32.0	36.0
37	34.347642942373426	36.0	36.0	36.0	32.0	36.0
38	33.910004957372365	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	15.0
22	58.0
23	166.0
24	458.0
25	1156.0
26	2433.0
27	4676.0
28	8193.0
29	13206.0
30	19680.0
31	28277.0
32	40606.0
33	63408.0
34	155843.0
35	355739.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.08909812308948	18.740424845032017	12.144791476004285	26.02568555587423
2	15.286144144248007	21.368868854443477	37.607577862450206	25.737409138858308
3	18.14326229687743	25.278275756719832	29.22976268020913	27.348699266193606
4	11.741767015027756	16.58860726658558	36.792781835265366	34.8768438831213
5	13.410701007038316	37.77834781155068	33.76619072049066	15.044760460920342
6	32.99150472320097	36.89500875467456	16.969945886744053	13.143540635380413
7	28.831959003565284	31.23585919869033	21.294137313845805	18.63804448389858
8	27.33784023323279	33.73185434767884	19.972214976531166	18.9580904425572
9	27.374865252805915	13.971338479186961	18.662990782427237	39.99080548557989
10	15.400811350256879	27.568182964526844	32.244039169626966	24.78696651558931
11	37.2838609446563	21.282869421601944	22.5570053697891	18.87626426395266
12	24.10241038514859	24.46254146767467	29.654971653547847	21.780076493628894
13	29.704935042476382	19.742619773315177	25.64564824221987	24.90679694198857
14	23.269098853463532	20.346266695104305	25.742741196340766	30.641893255091396
15	24.71970670801653	27.771949342571723	23.18782100427141	24.320522945140333
16	24.820583471198244	26.27810858951228	24.805019627735923	24.09628831155356
17	23.239268153494084	26.06669971581575	26.154750719107213	24.53928141158296
18	24.069051585494496	25.251183140322457	27.500014410966166	23.179750863216874
19	25.038621389332427	25.212417641328344	26.227237878936354	23.521723090402872
20	24.990921091313645	24.69405518823604	26.35290150392843	23.962122216521884
21	26.502419608193524	24.575811988229088	25.1551344979349	23.766633905642486
22	24.836074787365582	24.999207394576274	26.134218361353135	24.03049945670501
23	23.884591218533654	24.540578398538152	26.314136004934312	25.260694377993882
24	24.08288611301656	25.59589345108054	26.2923754460194	24.028844989883503
25	24.31767579602689	24.839929962603488	26.42557085522002	24.4168233861496
26	23.508753220850938	25.932677730445757	27.081664063085448	23.476904985617857
27	24.559492156820358	25.13708451323289	26.21617921503354	24.087244114913208
28	23.567405853158018	25.4416961130742	26.809152692833138	24.181745340934636
29	23.670732480588427	25.12768116025571	26.78004254117213	24.421543817983732
30	23.713388940448123	25.6620397857954	26.84028037975778	23.784290893998698
31	24.133468604269105	25.41244185175151	25.873016330506864	24.581073213472525
32	23.83501749491293	25.74187653837064	25.8104727373342	24.61263322938223
33	23.595795456510587	25.018590146357774	26.53592077427239	24.849693622859252
34	24.308561843220218	25.465474207252754	26.660863850956023	23.56510009857101
35	24.84277635909822	25.209391338432894	26.72600141803907	23.221830884429814
36	23.754171974705873	26.10229480225272	25.974613641997014	24.168919581044392
37	24.601969114417308	25.774445321912165	25.9577528115795	23.66583275209103
38	23.762962664068848	25.465041878267687	26.359818767689465	24.412176689974004
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	316.0
1	240.5
2	165.0
3	165.0
4	487.0
5	809.0
6	809.0
7	950.0
8	1091.0
9	1062.0
10	1033.0
11	1033.0
12	1321.0
13	1609.0
14	2002.0
15	2395.0
16	2395.0
17	3686.5
18	4978.0
19	4978.0
20	5529.0
21	6080.0
22	6451.0
23	6822.0
24	6822.0
25	8228.0
26	9634.0
27	9634.0
28	12380.5
29	15127.0
30	18136.0
31	21145.0
32	21145.0
33	25123.5
34	29102.0
35	29102.0
36	31098.0
37	33094.0
38	37178.5
39	41263.0
40	41263.0
41	43236.0
42	45209.0
43	49739.5
44	54270.0
45	54270.0
46	55908.0
47	57546.0
48	57546.0
49	59759.5
50	61973.0
51	61028.0
52	60083.0
53	60083.0
54	57055.5
55	54028.0
56	54028.0
57	52339.5
58	50651.0
59	44901.5
60	39152.0
61	39152.0
62	36987.0
63	34822.0
64	28893.0
65	22964.0
66	22964.0
67	19400.5
68	15837.0
69	15837.0
70	12604.5
71	9372.0
72	7237.0
73	5102.0
74	5102.0
75	3844.5
76	2587.0
77	2587.0
78	2547.0
79	2507.0
80	1919.0
81	1331.0
82	1331.0
83	1282.5
84	1234.0
85	1234.0
86	773.5
87	313.0
88	263.5
89	214.0
90	214.0
91	122.0
92	30.0
93	21.5
94	13.0
95	13.0
96	10.0
97	7.0
98	7.0
99	7.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009078908686353969
2	0.0
3	0.0
4	0.0
5	0.0
6	1.4410966168815822E-4
7	2.8821932337631643E-4
8	0.0024498642486986896
9	0.0034586318805157968
10	0.0015852062785697404
11	0.004899728497397379
12	2.8821932337631643E-4
13	1.4410966168815822E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	2.8821932337631643E-4
22	2.8821932337631643E-4
23	0.0
24	0.0
25	1.4410966168815822E-4
26	0.0
27	1.4410966168815822E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	693916.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.14884487558273
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.1930939504725	49.43819657871168
2	12.078844489370828	14.530570869349066
3	2.903245340597057	5.238805608819922
4	0.9959288699749917	2.3961588442896073
5	0.4533420127349883	1.3633999199790632
6	0.24135826656262951	0.871045256094909
7	0.17004663510110113	0.7159676073417667
8	0.12183533738414304	0.5862603846946484
9	0.07682397612399566	0.4158786080346918
>10	0.5849389811425092	7.0035880106695325
>50	0.08653498248161946	3.657982215755328
>100	0.08653479420840639	10.118660602422217
>500	0.006508187865181454	2.529121456169989
>1k	9.64175980026882E-4	1.1343640376676216
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3043	0.43852570051706546	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2410	0.3473042846684613	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1363	0.19642146888095968	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1008	0.1452625389816635	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	894	0.12883403754921346	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	852	0.1227814317583108	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	839	0.12090800615636474	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	754	0.1086586849128713	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	740	0.10664114964923709	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	730	0.1052000530323555	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	723	0.10419128540053839	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	720	0.10375895641547392	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	715	0.10303840810703313	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.4410966168815822E-4
7	0.0	0.0	0.0	0.0	1.4410966168815822E-4
8	0.0	0.0	0.0	0.0	1.4410966168815822E-4
9	0.0	0.0	0.0	0.0	1.4410966168815822E-4
10	0.0	0.0	0.0	0.0	1.4410966168815822E-4
11	0.0	0.0	0.0	0.0	1.4410966168815822E-4
12	0.0	0.0	0.0	0.0	8.646579701289493E-4
13	0.0	0.0	0.0	0.0	8.646579701289493E-4
14	0.0	0.0	0.0	0.0	8.646579701289493E-4
15	0.0	0.0	0.0	0.0	8.646579701289493E-4
16	0.0	0.0	0.0	2.8821932337631643E-4	8.646579701289493E-4
17	0.0	0.0	0.0	2.8821932337631643E-4	8.646579701289493E-4
18	0.0	0.0	0.0	2.8821932337631643E-4	8.646579701289493E-4
19	0.0	0.0	0.0	5.764386467526329E-4	8.646579701289493E-4
20	0.0	0.0	0.0	8.646579701289493E-4	0.0010087676318171076
21	0.0	0.0	0.0	0.0010087676318171076	0.0010087676318171076
22	0.0	0.0	0.0	0.0015852062785697404	0.0010087676318171076
23	0.0	0.0	0.0	0.0036027415422039554	0.0010087676318171076
24	0.0	0.0	0.0	0.005332057482461854	0.0011528772935052657
25	0.0	0.0	0.0	0.006773154099343436	0.0011528772935052657
26	0.0	0.0	0.0	0.008214250716225018	0.0011528772935052657
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGACGC	20	0.0037525406	31.999928	28
GCCGCGC	35	0.002065747	22.857092	8
AACTTAT	60	4.716205E-6	21.333284	7
AACGGAC	40	0.004481778	19.999954	15
ACTTATC	75	1.5068399E-6	19.199957	8
TCGAACT	50	7.1921165E-4	19.199957	19
TCGTTTA	195	0.0	18.871752	30
ATCGTTT	195	0.0	18.871752	29
CGTTTAT	195	0.0	18.871752	31
CATCGTT	200	0.0	18.399958	28
ATAACAC	80	2.7930037E-6	17.99996	3
ATTAGCT	90	4.3606633E-7	17.777739	3
ACCGCTG	45	0.0088469535	17.777739	8
TAGATAA	155	0.0	17.548347	4
ACTTATT	55	0.001363928	17.454506	8
GGCATCG	220	0.0	17.454506	26
TCTAGCG	185	0.0	17.297258	28
CTAGCGG	185	0.0	17.297258	29
CGCGTAA	65	2.0989291E-4	17.23073	10
ATATAAC	75	3.2307704E-5	17.06663	3
>>END_MODULE