FastQCFastQC Report
Thu 2 Feb 2017
SRR4063097_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063097_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1577301
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT28780.18246358811666258No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT27520.17447525868556477No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC25430.16122477574033112No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT21410.13573820088873334No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA21030.13332902217141812No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC20970.13294862553184206No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG20470.12977865353537468No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG20360.12908125969615186No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG20200.12806686865728228No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC19720.1250236955406736No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT18990.12039553642583121No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG18680.11843015378802144No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC17980.11399219299296709No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG17830.11304120139402687No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC17690.11215360923501601No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG17120.10853984115904321No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG16960.10752545012017364No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT16170.10251689436575517No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC16130.10226329660603778No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA16060.10181950052653235No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTA400.00448685719.997968
GTATCAA20400.019.309291
TCTAGCG4700.018.72268128
GGTATCA8200.018.3558861
CTAGCGG4800.018.33262429
TAGCGGC5200.017.23010330
GTCCTAT5700.016.2935051
TCCTATA908.564773E-616.0039482
AAGACGG6750.015.8788545
CGGTCCA5950.015.86644310
CAAGACG7100.015.3214084
TCTTGCG6100.015.2168682
ATCGTTT4350.015.07987829
CCTATTC6050.015.0728423
TTGTTCG859.43119E-515.0596739
CTATTCC5950.015.0563324
CGCCGGT5750.015.0245537
GCCGGTC6400.014.9984698
GCCCTAA650.00414079914.7808451
CTTGCGC6200.014.7081763
Produced by FastQC (version 0.11.5)