##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063097_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1577301
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.243470967177476	32.0	32.0	32.0	32.0	32.0
2	30.862111290108864	32.0	32.0	32.0	32.0	32.0
3	30.89979528320847	32.0	32.0	32.0	32.0	32.0
4	30.971882348391333	32.0	32.0	32.0	32.0	32.0
5	30.87062837086897	32.0	32.0	32.0	32.0	32.0
6	34.50982406021425	36.0	36.0	36.0	32.0	36.0
7	34.45491697526344	36.0	36.0	36.0	32.0	36.0
8	34.439678285882025	36.0	36.0	36.0	32.0	36.0
9	34.590813040757595	36.0	36.0	36.0	32.0	36.0
10	34.3018700932796	36.0	36.0	36.0	32.0	36.0
11	34.54947723991806	36.0	36.0	36.0	32.0	36.0
12	34.37180855144326	36.0	36.0	36.0	32.0	36.0
13	34.46358811666258	36.0	36.0	36.0	32.0	36.0
14	34.35275448376689	36.0	36.0	36.0	32.0	36.0
15	34.3001589423959	36.0	36.0	36.0	32.0	36.0
16	34.31596632475349	36.0	36.0	36.0	32.0	36.0
17	34.23748859602574	36.0	36.0	36.0	32.0	36.0
18	34.269569346624394	36.0	36.0	36.0	32.0	36.0
19	34.258210068972254	36.0	36.0	36.0	32.0	36.0
20	34.2497303938817	36.0	36.0	36.0	32.0	36.0
21	34.23348365340541	36.0	36.0	36.0	32.0	36.0
22	34.20632269934527	36.0	36.0	36.0	32.0	36.0
23	34.150066474312766	36.0	36.0	36.0	32.0	36.0
24	34.139202346286474	36.0	36.0	36.0	32.0	36.0
25	34.11519868433482	36.0	36.0	36.0	32.0	36.0
26	34.057415166794414	36.0	36.0	36.0	32.0	36.0
27	34.068626089757124	36.0	36.0	36.0	32.0	36.0
28	34.05697454068691	36.0	36.0	36.0	32.0	36.0
29	34.019160578735445	36.0	36.0	36.0	32.0	36.0
30	33.99609522849475	36.0	36.0	36.0	32.0	36.0
31	34.02304633040872	36.0	36.0	36.0	32.0	36.0
32	33.971028991929884	36.0	36.0	36.0	32.0	36.0
33	33.938671185778745	36.0	36.0	36.0	32.0	36.0
34	33.951368825607794	36.0	36.0	36.0	32.0	36.0
35	33.91383572317522	36.0	36.0	36.0	32.0	36.0
36	33.87276683397779	36.0	36.0	36.0	32.0	36.0
37	33.88636601384263	36.0	36.0	36.0	32.0	36.0
38	33.297214038411184	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	2.0
8	2.0
9	5.0
10	13.0
11	12.0
12	6.0
13	3.0
14	123.0
15	307.0
16	421.0
17	524.0
18	640.0
19	819.0
20	1231.0
21	1720.0
22	2861.0
23	4229.0
24	6681.0
25	10175.0
26	15395.0
27	22066.0
28	30986.0
29	42522.0
30	57104.0
31	76944.0
32	104860.0
33	157052.0
34	345484.0
35	695113.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.61937139940943	17.069052630509905	11.280813692209648	26.030762277871016
2	17.35843239941312	18.948944891539533	36.67522374312785	27.017398965919494
3	19.33005689488238	22.579336118580777	28.10992702516424	29.9806799613726
4	12.89763011721026	14.529203024756365	35.12015525747012	37.45301160056326
5	15.347268142905236	35.76929392498686	33.030431174344464	15.85300675776344
6	35.63740454385515	34.69896246421881	16.032701555511174	13.630931436414876
7	31.30469790147721	29.777024028402966	20.198947568629936	18.719330501489885
8	28.938974636957575	32.2819764216586	18.83452366234598	19.944525279037848
9	27.387405550504724	13.969966360193192	18.323804379388044	40.31882370991404
10	16.569781264482884	25.75025692753344	30.551457465417016	27.12850434256666
11	38.6448924317035	20.77098696043878	21.33601136642454	19.248109241433177
12	25.065128478564503	23.263974964289496	27.699939198626485	23.970957358519517
13	29.50651900154843	18.54304341816088	25.135978978899004	26.81445860139168
14	23.88609623905308	19.356423841395525	24.14364844061082	32.613831478940575
15	25.621284326874598	26.662671574922236	21.5954367174297	26.120607380773464
16	26.655629768935295	25.283457207821275	22.88429518354179	25.17661783970164
17	24.853993260544506	25.590843776276458	24.182901263299136	25.372261699879893
18	25.964429977422398	24.039469271068782	25.292364383820466	24.703736367688354
19	26.134553736279344	24.44261417818086	24.750060867977357	24.67277121756244
20	26.308338035670133	23.670675805050625	24.157415215282487	25.863570943996756
21	27.634010797581798	23.64442161925685	23.86893270056841	24.85263488259294
22	26.598829191713673	23.4687271945681	24.46650743944494	25.465936174273285
23	24.860127622448566	23.321658280241852	25.307009089802378	26.511205007507204
24	25.26388942609796	24.541605662613396	24.644263674472015	25.550241236816625
25	25.327162302177612	23.972287428521426	24.734725556515155	25.9658247127858
26	25.27361871691528	25.057387007835068	25.06524997907435	24.603744296175297
27	26.067427983760876	23.929560309895074	24.277016115482137	25.72599559086191
28	24.98674876934743	23.99506217299894	25.42885801021042	25.58933104744321
29	24.92987306136424	24.362199931521616	25.187618093511038	25.520308913603106
30	24.697291889362706	24.66508417751775	25.521631789772403	25.115992143347142
31	25.691806613065722	24.455256779213435	24.077754973811366	25.775181633909476
32	25.42025238285146	24.05372281507754	24.002936837079723	26.523087964991277
33	24.92248794362575	23.78279124560765	24.887995871096024	26.40672493967057
34	26.050095141837744	23.937255210291678	24.843908417817552	25.168741230053026
35	26.443833177346086	23.678166604525405	25.094923946705627	24.783076271422882
36	24.819176113936646	24.721654544105682	24.555588880004716	25.903580461952956
37	26.26140812064171	24.241392472320605	24.40700598415132	25.090193422886365
38	25.113903665333087	23.855478978702898	25.04631596299795	25.98430139296606
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	24.0
1	53.0
2	82.0
3	82.0
4	265.5
5	449.0
6	449.0
7	547.0
8	645.0
9	640.0
10	635.0
11	635.0
12	945.0
13	1255.0
14	1815.0
15	2375.0
16	2375.0
17	3615.5
18	4856.0
19	4856.0
20	6094.0
21	7332.0
22	8174.5
23	9017.0
24	9017.0
25	10566.0
26	12115.0
27	12115.0
28	15530.0
29	18945.0
30	23676.5
31	28408.0
32	28408.0
33	37655.0
34	46902.0
35	46902.0
36	53504.5
37	60107.0
38	69377.5
39	78648.0
40	78648.0
41	85272.5
42	91897.0
43	105709.0
44	119521.0
45	119521.0
46	122722.5
47	125924.0
48	125924.0
49	135855.0
50	145786.0
51	151321.0
52	156856.0
53	156856.0
54	150058.5
55	143261.0
56	143261.0
57	139316.0
58	135371.0
59	121625.5
60	107880.0
61	107880.0
62	103061.0
63	98242.0
64	82173.5
65	66105.0
66	66105.0
67	55829.0
68	45553.0
69	45553.0
70	36401.0
71	27249.0
72	21012.5
73	14776.0
74	14776.0
75	11234.0
76	7692.0
77	7692.0
78	7468.0
79	7244.0
80	5658.5
81	4073.0
82	4073.0
83	4082.0
84	4091.0
85	4091.0
86	2752.0
87	1413.0
88	1165.5
89	918.0
90	918.0
91	666.0
92	414.0
93	365.5
94	317.0
95	317.0
96	283.0
97	249.0
98	249.0
99	461.5
100	674.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11823995546823339
2	0.05249473626149987
3	0.011538698067141275
4	0.003169971996467383
5	2.5359775971739066E-4
6	3.8039663957608596E-4
7	6.339943992934766E-5
8	3.8039663957608596E-4
9	1.9019831978804298E-4
10	5.071955194347813E-4
11	0.0015215865583043438
12	0.0021555809575978207
13	0.013884477344527137
14	0.009192918789755412
15	0.023204195014141244
16	0.01058770646820106
17	0.020224421337461905
18	0.004691558554771728
19	0.007417734471733677
20	0.004501360234983685
21	0.006086346233217375
22	0.006339943992934766
23	0.009446516549472801
24	0.013947876784456486
25	0.016166857181983657
26	0.018322438139581475
27	0.007734731671380416
28	0.005642550153711942
29	0.010207309828624974
30	0.002472578157244559
31	0.00570594959364129
32	0.005959547353358681
33	0.008051728871027154
34	0.011538698067141275
35	0.01578646054240757
36	0.013567480144880401
37	0.008558924390461934
38	0.005769349033570638
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1577301.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.49695054928143
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.63386409196941	43.13665839925568
2	11.601771167972709	12.413187569142302
3	3.324133954694582	5.334930893804501
4	1.4184934848490482	3.035403032537897
5	0.7704936742021438	2.060953099366312
6	0.48812483024927467	1.5667913943433105
7	0.3139616681011737	1.1757194282944878
8	0.23701264907732894	1.0143563173795247
9	0.17004346002117615	0.818712591478342
>10	0.876987184029831	8.250571740835374
>50	0.06971416570603993	2.6198813880834955
>100	0.0762723717658068	8.892297454023975
>500	0.013305945990716675	4.891399171767349
>1k	0.005821351370938545	4.7891375196874435
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2878	0.18246358811666258	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2752	0.17447525868556477	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2543	0.16122477574033112	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2141	0.13573820088873334	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2103	0.13332902217141812	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2097	0.13294862553184206	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2047	0.12977865353537468	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2036	0.12908125969615186	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2020	0.12806686865728228	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1972	0.1250236955406736	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1899	0.12039553642583121	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1868	0.11843015378802144	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1798	0.11399219299296709	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1783	0.11304120139402687	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1769	0.11215360923501601	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1712	0.10853984115904321	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1696	0.10752545012017364	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1617	0.10251689436575517	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1613	0.10226329660603778	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1606	0.10181950052653235	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	6.339943992934766E-5
6	0.0	0.0	0.0	0.0	6.339943992934766E-5
7	0.0	0.0	0.0	0.0	6.339943992934766E-5
8	0.0	0.0	0.0	0.0	6.339943992934766E-5
9	0.0	0.0	0.0	0.0	6.339943992934766E-5
10	0.0	0.0	0.0	0.0	1.9019831978804298E-4
11	0.0	0.0	0.0	0.0	1.9019831978804298E-4
12	0.0	0.0	0.0	0.0	3.8039663957608596E-4
13	0.0	0.0	0.0	0.0	5.071955194347813E-4
14	0.0	0.0	0.0	0.0	5.705949593641289E-4
15	0.0	0.0	0.0	0.0	0.0010143910388695626
16	0.0	0.0	0.0	6.339943992934766E-5	0.0010143910388695626
17	0.0	0.0	0.0	6.339943992934766E-5	0.0010143910388695626
18	0.0	0.0	0.0	6.339943992934766E-5	0.0012045893586576056
19	0.0	0.0	0.0	6.339943992934766E-5	0.0012045893586576056
20	0.0	0.0	0.0	6.339943992934766E-5	0.0013313882385163009
21	0.0	0.0	0.0	1.2679887985869533E-4	0.0014581871183749963
22	0.0	0.0	0.0	3.8039663957608596E-4	0.0015215865583043438
23	0.0	0.0	0.0	5.071955194347813E-4	0.0015215865583043438
24	0.0	0.0	0.0	6.339943992934767E-4	0.0015215865583043438
25	0.0	0.0	0.0	8.241927190815197E-4	0.0015215865583043438
26	0.0	0.0	0.0	0.0011411899187282579	0.0016483854381630393
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTTA	40	0.004486857	19.99796	8
GTATCAA	2040	0.0	19.30929	1
TCTAGCG	470	0.0	18.722681	28
GGTATCA	820	0.0	18.355886	1
CTAGCGG	480	0.0	18.332624	29
TAGCGGC	520	0.0	17.230103	30
GTCCTAT	570	0.0	16.293505	1
TCCTATA	90	8.564773E-6	16.003948	2
AAGACGG	675	0.0	15.878854	5
CGGTCCA	595	0.0	15.866443	10
CAAGACG	710	0.0	15.321408	4
TCTTGCG	610	0.0	15.216868	2
ATCGTTT	435	0.0	15.079878	29
CCTATTC	605	0.0	15.072842	3
TTGTTCG	85	9.43119E-5	15.059673	9
CTATTCC	595	0.0	15.056332	4
CGCCGGT	575	0.0	15.024553	7
GCCGGTC	640	0.0	14.998469	8
GCCCTAA	65	0.004140799	14.780845	1
CTTGCGC	620	0.0	14.708176	3
>>END_MODULE