FastQCFastQC Report
Thu 2 Feb 2017
SRR4063097_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063097_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1577301
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT28050.1778354290018202No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC27170.1722562782880376No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT23330.1479108933551681No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC22750.14423372583926594No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG22360.1417611476820214No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC22320.141507549922304No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT21750.13789378184633116No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA21390.13561140200887464No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG20230.12825706697707034No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC19900.12616488545940185No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG19540.12388250562194533No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG19040.12071253362547796No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA17430.11050522379685297No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG17050.10809604507953778No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG16960.10752545012017364No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC16720.1060038635618693No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA16550.10492607308307039No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG16420.10410188036398887No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG16410.10403848092405953No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG16090.1020096988463204No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC15970.10124890556716822No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG400.004481869320.0018025
GTAAGAC1450.018.7597183
TCTAGCG4700.018.72271728
GTATCAA20500.017.7197931
CTAGCGG5050.017.42510429
AGTATAC652.1000898E-417.2317773
CAAGACG6450.016.8702724
ATCGTTT5050.016.47464429
ACCGTCG5150.016.4675058
TACCGTC5500.016.2923777
CATCGTT5250.016.15178928
TAGACCC600.002440460716.0019514
AAGACGG7000.016.0014425
TAGCGGC5600.015.999413530
ATACCGT5650.015.8598376
GTATTAG4150.015.8096561
AGCGAAA6800.015.76412817
TCGTTTA5300.015.69753830
GGTATCA9300.015.65831851
CGAAAGC6800.015.52884219
Produced by FastQC (version 0.11.5)