##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063097_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1577301
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.191747802099915	32.0	32.0	32.0	32.0	32.0
2	31.22520685652263	32.0	32.0	32.0	32.0	32.0
3	31.325111693963297	32.0	32.0	32.0	32.0	32.0
4	31.43276964891292	32.0	32.0	32.0	32.0	32.0
5	31.34588261847295	32.0	32.0	32.0	32.0	32.0
6	34.86472588301155	36.0	36.0	36.0	32.0	36.0
7	34.887173088712935	36.0	36.0	36.0	32.0	36.0
8	34.81697342485676	36.0	36.0	36.0	32.0	36.0
9	34.941212235331115	36.0	36.0	36.0	32.0	36.0
10	34.77612072774949	36.0	36.0	36.0	32.0	36.0
11	34.95789009199893	36.0	36.0	36.0	36.0	36.0
12	34.84611307543709	36.0	36.0	36.0	32.0	36.0
13	34.915304054203986	36.0	36.0	36.0	32.0	36.0
14	34.853054046120555	36.0	36.0	36.0	32.0	36.0
15	34.81854699895581	36.0	36.0	36.0	32.0	36.0
16	34.83664436908364	36.0	36.0	36.0	32.0	36.0
17	34.79359741736042	36.0	36.0	36.0	32.0	36.0
18	34.80016686732589	36.0	36.0	36.0	32.0	36.0
19	34.79014214788427	36.0	36.0	36.0	32.0	36.0
20	34.782028287562106	36.0	36.0	36.0	32.0	36.0
21	34.77194587463014	36.0	36.0	36.0	32.0	36.0
22	34.76003185187862	36.0	36.0	36.0	32.0	36.0
23	34.70061579876003	36.0	36.0	36.0	32.0	36.0
24	34.67452566124031	36.0	36.0	36.0	32.0	36.0
25	34.66012384446596	36.0	36.0	36.0	32.0	36.0
26	34.589737152261996	36.0	36.0	36.0	32.0	36.0
27	34.57444520735104	36.0	36.0	36.0	32.0	36.0
28	34.54879569593882	36.0	36.0	36.0	32.0	36.0
29	34.518577620885296	36.0	36.0	36.0	32.0	36.0
30	34.49001617319713	36.0	36.0	36.0	32.0	36.0
31	34.48792018771306	36.0	36.0	36.0	32.0	36.0
32	34.46435398189693	36.0	36.0	36.0	32.0	36.0
33	34.42838621163621	36.0	36.0	36.0	32.0	36.0
34	34.430593780134544	36.0	36.0	36.0	32.0	36.0
35	34.40548950390572	36.0	36.0	36.0	32.0	36.0
36	34.383865222934624	36.0	36.0	36.0	32.0	36.0
37	34.37370419469715	36.0	36.0	36.0	32.0	36.0
38	33.92526030225049	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	4.0
21	26.0
22	101.0
23	386.0
24	1061.0
25	2550.0
26	5430.0
27	10944.0
28	18685.0
29	30243.0
30	45908.0
31	66659.0
32	96489.0
33	156142.0
34	370106.0
35	772566.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.98368975277206	18.38535907398578	11.987435707546691	26.643515465695472
2	16.257074585003117	19.999606923472438	36.005683125795265	27.73763536572918
3	18.213454502342927	23.46108954473496	28.384499851328314	29.940956101593798
4	12.335842687938488	15.560154137011523	35.16856041153923	36.935442763510764
5	14.753747065398423	36.30771805761868	32.721528738015124	16.21700613896777
6	35.63672097888798	35.1139288657833	15.726241044823434	13.523109110505294
7	31.177680776265692	30.002757886127835	20.069929848252862	18.749631489353607
8	28.789589640360745	32.692016293924645	18.57082626682094	19.94756779889366
9	27.313321363177568	13.842901849794833	18.092814356071557	40.75096243095604
10	16.318599107324	26.136386691012376	30.404430411848242	27.140583789815377
11	38.76117865237997	20.710615162706652	21.241294044920668	19.286912139992708
12	24.970645966261248	23.400173461417513	27.59310871263225	24.036071859688988
13	29.532910077867342	18.553881384494243	25.126640622127205	26.78656791551121
14	23.88624618890117	19.320408723509335	24.035171473295204	32.758173614294286
15	25.675505182587216	26.877051368128214	21.371127007464015	26.076316441820552
16	26.69885246940975	25.415773790654917	22.635579788245735	25.249793951689597
17	24.84028096095799	25.757163661216214	24.008670507404737	25.393884870421058
18	26.02914725851312	24.140921739097358	25.141174702862674	24.68875629952685
19	26.12424641840714	24.54705855128476	24.55840705103211	24.77028797927599
20	26.402379761377187	23.611473016247373	23.944573673636167	26.041573548739272
21	27.73884690074222	23.721974999001453	23.64494448413964	24.894233616116683
22	26.50039529550796	23.67144888642054	24.335494620240524	25.49266119783098
23	24.841802547516295	23.418041325022934	25.153854590848546	26.58630153661223
24	25.334796592406907	24.51104766940489	24.509399283966726	25.644756454221486
25	25.427756292398403	24.00760793761491	24.602104862740127	25.96253090724656
26	25.352104639507616	25.130523596954546	24.853341245583437	24.664030517954405
27	26.22842832688772	23.971977429785078	24.049895390857795	25.74969885246941
28	25.08519299740506	24.11365997992774	25.24388179554822	25.557265227118986
29	25.066109765986326	24.34183456423346	25.043095769291973	25.54895990048824
30	24.870142097164713	24.611472382252973	25.301448487004063	25.216937033578247
31	25.64608784246	24.456080354986142	23.966383080971863	25.931448721581994
32	25.533680635465267	24.090392385473667	23.80433411251245	26.571592866548617
33	24.937916098449186	23.8575896420531	24.726478966284812	26.4780152932129
34	25.968600793380592	24.00531033708848	24.72654236572474	25.299546503806187
35	26.46932956994258	23.77884753766085	24.827093877452686	24.924729014943882
36	25.01900398211882	24.71994882397209	24.342024762553248	25.91902243135584
37	26.418356420239387	24.333656036482573	24.103389270659186	25.14459827261886
38	25.1550591802072	23.911479165993047	24.85613082094033	26.077330832859424
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14.0
1	32.0
2	50.0
3	50.0
4	147.0
5	244.0
6	244.0
7	364.0
8	484.0
9	535.0
10	586.0
11	586.0
12	808.5
13	1031.0
14	1598.5
15	2166.0
16	2166.0
17	3325.0
18	4484.0
19	4484.0
20	5537.5
21	6591.0
22	7739.5
23	8888.0
24	8888.0
25	10622.5
26	12357.0
27	12357.0
28	15765.5
29	19174.0
30	23904.0
31	28634.0
32	28634.0
33	38187.0
34	47740.0
35	47740.0
36	53771.5
37	59803.0
38	69615.5
39	79428.0
40	79428.0
41	85819.5
42	92211.0
43	106231.5
44	120252.0
45	120252.0
46	122791.5
47	125331.0
48	125331.0
49	135700.5
50	146070.0
51	151288.5
52	156507.0
53	156507.0
54	150156.0
55	143805.0
56	143805.0
57	140190.5
58	136576.0
59	122731.5
60	108887.0
61	108887.0
62	103483.5
63	98080.0
64	82003.0
65	65926.0
66	65926.0
67	55968.5
68	46011.0
69	46011.0
70	36802.5
71	27594.0
72	21251.0
73	14908.0
74	14908.0
75	11181.5
76	7455.0
77	7455.0
78	7204.0
79	6953.0
80	5387.0
81	3821.0
82	3821.0
83	3660.5
84	3500.0
85	3500.0
86	2203.5
87	907.0
88	763.0
89	619.0
90	619.0
91	374.5
92	130.0
93	88.0
94	46.0
95	46.0
96	30.0
97	14.0
98	14.0
99	19.0
100	24.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008432125510603239
2	0.0
3	0.0
4	1.9019831978804298E-4
5	0.0
6	6.339943992934766E-5
7	3.8039663957608596E-4
8	0.0016483854381630393
9	0.002852974796820645
10	0.001331388238516301
11	0.0048183574346304225
12	3.1699719964673834E-4
13	1.9019831978804298E-4
14	0.0
15	0.0
16	6.339943992934766E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	2.5359775971739066E-4
22	0.0
23	0.0
24	0.0
25	6.339943992934766E-5
26	0.0
27	6.339943992934766E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1577301.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.65752713424916
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.32831444161963	40.979045557822154
2	12.234761163076618	12.640350135253767
3	3.616283742960561	5.604248267313879
4	1.577669662645935	3.2599405362805642
5	0.840086965993804	2.1698407620476994
6	0.5181324024894547	1.6059263184439627
7	0.3540358715852844	1.2802032350040065
8	0.24680402429372145	1.0199428465435842
9	0.16916684343348834	0.7864866724392633
>10	0.9406982408015006	8.581707306288337
>50	0.07318823299009299	2.6712237508030974
>100	0.07977445337149529	9.01439952376263
>500	0.01442586376846629	5.123057662842052
>1k	0.006658090970061364	5.263627425155158
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2805	0.1778354290018202	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2717	0.1722562782880376	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2333	0.1479108933551681	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2275	0.14423372583926594	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2236	0.1417611476820214	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2232	0.141507549922304	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2175	0.13789378184633116	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2139	0.13561140200887464	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2023	0.12825706697707034	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1990	0.12616488545940185	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1954	0.12388250562194533	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1904	0.12071253362547796	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1743	0.11050522379685297	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1705	0.10809604507953778	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1696	0.10752545012017364	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1672	0.1060038635618693	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1655	0.10492607308307039	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1642	0.10410188036398887	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	1641	0.10403848092405953	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1609	0.1020096988463204	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	1597	0.10124890556716822	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2679887985869533E-4
2	0.0	0.0	0.0	0.0	1.9019831978804298E-4
3	0.0	0.0	0.0	0.0	1.9019831978804298E-4
4	0.0	0.0	0.0	0.0	1.9019831978804298E-4
5	0.0	0.0	0.0	0.0	1.9019831978804298E-4
6	0.0	0.0	0.0	0.0	2.5359775971739066E-4
7	0.0	0.0	0.0	0.0	2.5359775971739066E-4
8	0.0	0.0	0.0	0.0	2.5359775971739066E-4
9	0.0	0.0	0.0	0.0	2.5359775971739066E-4
10	0.0	0.0	0.0	0.0	2.5359775971739066E-4
11	0.0	0.0	0.0	0.0	2.5359775971739066E-4
12	0.0	0.0	0.0	0.0	5.705949593641289E-4
13	0.0	0.0	0.0	0.0	6.339943992934767E-4
14	0.0	0.0	0.0	0.0	6.339943992934767E-4
15	0.0	0.0	0.0	0.0	0.0010777904787989104
16	0.0	0.0	0.0	6.339943992934766E-5	0.0010777904787989104
17	0.0	0.0	0.0	1.2679887985869533E-4	0.0010777904787989104
18	0.0	0.0	0.0	1.2679887985869533E-4	0.0011411899187282579
19	0.0	0.0	0.0	1.2679887985869533E-4	0.0011411899187282579
20	0.0	0.0	0.0	1.9019831978804298E-4	0.0012679887985869534
21	0.0	0.0	0.0	1.9019831978804298E-4	0.0012679887985869534
22	0.0	0.0	0.0	3.1699719964673834E-4	0.0012679887985869534
23	0.0	0.0	0.0	4.4379607950543364E-4	0.0012679887985869534
24	0.0	0.0	0.0	5.705949593641289E-4	0.0012679887985869534
25	0.0	0.0	0.0	8.241927190815197E-4	0.0012679887985869534
26	0.0	0.0	0.0	0.0011411899187282579	0.0015215865583043438
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	40	0.0044818693	20.001802	5
GTAAGAC	145	0.0	18.759718	3
TCTAGCG	470	0.0	18.722717	28
GTATCAA	2050	0.0	17.719793	1
CTAGCGG	505	0.0	17.425104	29
AGTATAC	65	2.1000898E-4	17.231777	3
CAAGACG	645	0.0	16.870272	4
ATCGTTT	505	0.0	16.474644	29
ACCGTCG	515	0.0	16.467505	8
TACCGTC	550	0.0	16.292377	7
CATCGTT	525	0.0	16.151789	28
TAGACCC	60	0.0024404607	16.001951	4
AAGACGG	700	0.0	16.001442	5
TAGCGGC	560	0.0	15.9994135	30
ATACCGT	565	0.0	15.859837	6
GTATTAG	415	0.0	15.809656	1
AGCGAAA	680	0.0	15.764128	17
TCGTTTA	530	0.0	15.697538	30
GGTATCA	930	0.0	15.6583185	1
CGAAAGC	680	0.0	15.528842	19
>>END_MODULE