FastQCFastQC Report
Thu 2 Feb 2017
SRR4063095_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063095_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences855845
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT31040.3626824950779639No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21350.24946105895343199No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT11640.13600593565423644No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9680.11310459253720008No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA9000.10515922859863644No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG8690.10153707739135005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG902.0396328E-819.553024
CTAGTAT507.1831915E-419.2042433
AGGACGT904.372123E-717.7744355
CGATTTG1800.015.99979528
GCGATTT1900.015.99979427
AACGCTT2000.015.19980630
GAGTGAT951.4325071E-515.15681511
GTCTAGT859.339839E-515.0753691
AAACGCT2100.014.47600629
CGTTATT1002.315641E-514.4057092
TCTATGC901.5305827E-414.2253653
AGCGATT2250.014.2220426
CTATTCC2250.014.2203784
TAAACGC2150.014.13935528
AACCGCG1258.5947795E-714.0781747
ACCGTCG1952.910383E-1113.9469118
ATTATAC1155.5969413E-613.9161183
GTAAACG2300.013.91286527
GTATAGC1503.119567E-813.8819031
TAAGAGG3000.013.8648694
Produced by FastQC (version 0.11.5)