##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063095_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	855845
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21651467263348	32.0	32.0	32.0	32.0	32.0
2	30.86491829712156	32.0	32.0	32.0	32.0	32.0
3	30.923634536627542	32.0	32.0	32.0	32.0	32.0
4	30.969169651046627	32.0	32.0	32.0	32.0	32.0
5	30.91132740157388	32.0	32.0	32.0	32.0	32.0
6	34.557227067985444	36.0	36.0	36.0	32.0	36.0
7	34.4842021627748	36.0	36.0	36.0	32.0	36.0
8	34.45895343198827	36.0	36.0	36.0	32.0	36.0
9	34.58443993947502	36.0	36.0	36.0	32.0	36.0
10	34.33431053520205	36.0	36.0	36.0	32.0	36.0
11	34.5897306171094	36.0	36.0	36.0	32.0	36.0
12	34.41480875625844	36.0	36.0	36.0	32.0	36.0
13	34.48858613417149	36.0	36.0	36.0	32.0	36.0
14	34.39289824676197	36.0	36.0	36.0	32.0	36.0
15	34.359271830763745	36.0	36.0	36.0	32.0	36.0
16	34.369105387073596	36.0	36.0	36.0	32.0	36.0
17	34.30101128124836	36.0	36.0	36.0	32.0	36.0
18	34.32713166519638	36.0	36.0	36.0	32.0	36.0
19	34.31283702072221	36.0	36.0	36.0	32.0	36.0
20	34.26519287955179	36.0	36.0	36.0	32.0	36.0
21	34.259853127610725	36.0	36.0	36.0	32.0	36.0
22	34.236338355660195	36.0	36.0	36.0	32.0	36.0
23	34.18779919261081	36.0	36.0	36.0	32.0	36.0
24	34.18015528512756	36.0	36.0	36.0	32.0	36.0
25	34.159646898679085	36.0	36.0	36.0	32.0	36.0
26	34.11006081708721	36.0	36.0	36.0	32.0	36.0
27	34.117582038803754	36.0	36.0	36.0	32.0	36.0
28	34.10046679013139	36.0	36.0	36.0	32.0	36.0
29	34.06952076602656	36.0	36.0	36.0	32.0	36.0
30	34.03618762743254	36.0	36.0	36.0	32.0	36.0
31	34.05071712751725	36.0	36.0	36.0	32.0	36.0
32	34.019214927936716	36.0	36.0	36.0	32.0	36.0
33	33.967185646933736	36.0	36.0	36.0	32.0	36.0
34	33.96269651630844	36.0	36.0	36.0	32.0	36.0
35	33.91653862556888	36.0	36.0	36.0	32.0	36.0
36	33.8896061786889	36.0	36.0	36.0	32.0	36.0
37	33.87216727327962	36.0	36.0	36.0	32.0	36.0
38	33.277960378339536	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	0.0
9	5.0
10	5.0
11	5.0
12	1.0
13	1.0
14	137.0
15	282.0
16	346.0
17	415.0
18	532.0
19	691.0
20	849.0
21	1219.0
22	1786.0
23	2552.0
24	3779.0
25	5599.0
26	8208.0
27	11516.0
28	16274.0
29	22078.0
30	29351.0
31	39657.0
32	53850.0
33	80531.0
34	179952.0
35	396220.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.57451418036507	17.175297481177854	11.428050030180946	25.82213830827613
2	16.310347044828816	20.234636675571245	37.935618127933694	25.519398151666252
3	18.552856834023757	25.014869910196257	28.667200308499513	27.765072947280473
4	12.48384601542837	15.786853869828679	36.4745871240182	35.25471299072475
5	14.249342461175708	36.884101406449545	33.82512914167668	15.04142699069806
6	33.04722133290958	36.1883385017328	17.46817753744266	13.296262627914965
7	29.45661889711338	30.872178957638358	21.59561602860331	18.075586116644953
8	27.90261275166766	32.79464293017044	20.12745358074689	19.17529073741501
9	27.916582441323662	14.055914722278374	18.715085430787802	39.31241740561017
10	15.539254462579994	27.043988413708654	32.222181975815545	25.194575147895808
11	36.87714046347936	21.43448881847	22.629584579198465	19.05878613885218
12	24.651885607454798	24.169777699880232	29.244530131744224	21.93380656092075
13	29.67785738408557	19.8607285632652	25.228954155414208	25.23245989723502
14	23.67089864860917	19.96845001957267	25.768271236350255	30.592380095467902
15	24.874015968884983	26.998447988930234	23.231431963948467	24.896104078236316
16	25.099589023647788	25.952858663631574	24.350436509003035	24.5971158037176
17	23.86068286571741	25.75483120015707	25.694295116911768	24.69019081721375
18	24.565966744955073	24.953669622930324	26.85479253572639	23.625571096388217
19	25.125965187802645	25.249009105016174	26.207886963187317	23.417138743993867
20	25.426735218509002	24.191516709511568	25.940289787333487	24.441458284645947
21	26.68785529413827	24.366181548366743	24.916423718019875	24.029539439475116
22	25.56032694745821	24.541391337878814	25.36366770079283	24.534614013870144
23	24.12232936194478	24.43887972254811	25.8669203120399	25.57187060346721
24	24.202682110537733	25.458314636152867	25.927613865829034	24.411389387480366
25	24.594959075207196	24.60769694618308	25.7714007918515	25.025943186758226
26	24.2291902100174	25.56448531070211	26.190996516265802	24.015327963014688
27	24.953025749367836	25.107503260153212	25.302177642126324	24.637293348352628
28	24.23183704756207	24.99509235716422	26.15095909362848	24.62211150164523
29	24.19147514011003	25.32567694529749	26.02108544959077	24.46176246500171
30	24.160309223540903	25.398418594542548	26.369182340699748	24.072089841216794
31	24.62441794567688	25.208429490362878	25.26767195798108	24.899480605979164
32	24.193064025548054	25.424778481863147	25.192013545236673	25.190143947352123
33	23.903060330221198	25.04855163065705	25.7547996587948	25.29358838032695
34	24.63304762405567	25.170812080419275	25.982366654396944	24.213773641128114
35	25.352242702438033	24.976189947541926	25.778077456583905	23.893489893436136
36	24.287330290303206	25.71874642116752	25.51809808279753	24.47582520573174
37	24.990242796613142	25.59402087924672	25.37176697694963	24.04396934719051
38	24.135287828418857	25.69615741903822	25.68120074082297	24.487354011719958
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	264.0
1	239.5
2	215.0
3	215.0
4	557.0
5	899.0
6	899.0
7	1034.0
8	1169.0
9	1122.0
10	1075.0
11	1075.0
12	1378.0
13	1681.0
14	2112.0
15	2543.0
16	2543.0
17	3779.5
18	5016.0
19	5016.0
20	5949.5
21	6883.0
22	7147.5
23	7412.0
24	7412.0
25	8400.0
26	9388.0
27	9388.0
28	11973.0
29	14558.0
30	17747.5
31	20937.0
32	20937.0
33	25885.5
34	30834.0
35	30834.0
36	34277.5
37	37721.0
38	42779.5
39	47838.0
40	47838.0
41	51789.5
42	55741.0
43	62112.0
44	68483.0
45	68483.0
46	72043.5
47	75604.0
48	75604.0
49	78167.0
50	80730.0
51	79825.0
52	78920.0
53	78920.0
54	75322.5
55	71725.0
56	71725.0
57	68911.0
58	66097.0
59	58729.5
60	51362.0
61	51362.0
62	47676.5
63	43991.0
64	36268.5
65	28546.0
66	28546.0
67	23379.5
68	18213.0
69	18213.0
70	14681.0
71	11149.0
72	8636.5
73	6124.0
74	6124.0
75	4667.0
76	3210.0
77	3210.0
78	3033.0
79	2856.0
80	2166.0
81	1476.0
82	1476.0
83	1515.0
84	1554.0
85	1554.0
86	1019.5
87	485.0
88	433.5
89	382.0
90	382.0
91	263.5
92	145.0
93	136.5
94	128.0
95	128.0
96	117.5
97	107.0
98	107.0
99	245.5
100	384.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11696043091915008
2	0.056084921919272765
3	0.010515922859863644
4	0.0022200281593045467
5	2.336871746636365E-4
6	3.505307619954548E-4
7	0.0
8	4.67374349327273E-4
9	7.010615239909096E-4
10	7.010615239909096E-4
11	0.0016358102226454558
12	0.002336871746636365
13	0.012385420257172736
14	0.007010615239909096
15	0.021148689307059106
16	0.009113799811881824
17	0.018344443211095466
18	0.004089525556613639
19	0.006192710128586368
20	0.005257961429931822
21	0.00467374349327273
22	0.005842179366590913
23	0.006660084477913641
24	0.011801202320513644
25	0.015072822765804555
26	0.017993912449100015
27	0.006660084477913641
28	0.004323212731277276
29	0.010399079272531826
30	0.0028042460959636383
31	0.00467374349327273
32	0.00537480501726364
33	0.006426397303250005
34	0.00934748698654546
35	0.013320168955827282
36	0.013904386892486372
37	0.007828520351231823
38	0.00467374349327273
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	855845.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.465130837561325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.15636121290213	51.3190785230188
2	11.610382368313722	14.504881074216636
3	3.0395609024466586	5.695997083801994
4	1.140339379315409	2.8492579411264196
5	0.5667371574001561	1.7700655343754166
6	0.3173105676571103	1.189250767490535
7	0.22655411255519037	0.9906212597783293
8	0.1465757366552257	0.7324698054224485
9	0.11215984194425854	0.6305471281591477
>10	0.5445436006856429	6.331356715036258
>50	0.06842349569686	3.016484625954051
>100	0.06503667211825018	7.926736213394081
>500	0.005451050530420419	2.2914515202614876
>1k	5.639017790090089E-4	0.7518018079644551
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3104	0.3626824950779639	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2135	0.24946105895343199	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1164	0.13600593565423644	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	968	0.11310459253720008	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	900	0.10515922859863644	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	869	0.10153707739135005	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.1684358733181826E-4
7	0.0	0.0	0.0	0.0	2.3368717466363653E-4
8	0.0	0.0	0.0	0.0	2.3368717466363653E-4
9	0.0	0.0	0.0	1.1684358733181826E-4	2.3368717466363653E-4
10	0.0	0.0	0.0	1.1684358733181826E-4	2.3368717466363653E-4
11	0.0	0.0	0.0	1.1684358733181826E-4	2.3368717466363653E-4
12	0.0	0.0	0.0	2.3368717466363653E-4	3.505307619954548E-4
13	0.0	0.0	0.0	2.3368717466363653E-4	3.505307619954548E-4
14	0.0	0.0	0.0	2.3368717466363653E-4	3.505307619954548E-4
15	0.0	0.0	0.0	2.3368717466363653E-4	3.505307619954548E-4
16	0.0	0.0	0.0	2.3368717466363653E-4	4.6737434932727306E-4
17	0.0	0.0	0.0	3.505307619954548E-4	4.6737434932727306E-4
18	0.0	0.0	0.0	3.505307619954548E-4	4.6737434932727306E-4
19	0.0	0.0	0.0	3.505307619954548E-4	4.6737434932727306E-4
20	0.0	0.0	0.0	5.842179366590913E-4	4.6737434932727306E-4
21	0.0	0.0	0.0	7.010615239909096E-4	4.6737434932727306E-4
22	0.0	0.0	0.0	0.0012852794606500008	4.6737434932727306E-4
23	0.0	0.0	0.0	0.0019863409846409105	4.6737434932727306E-4
24	0.0	0.0	0.0	0.0033884640326227295	4.6737434932727306E-4
25	0.0	0.0	0.0	0.004206369143945458	4.6737434932727306E-4
26	0.0	0.0	0.0	0.005608492191927277	5.842179366590913E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACG	90	2.0396328E-8	19.55302	4
CTAGTAT	50	7.1831915E-4	19.204243	3
AGGACGT	90	4.372123E-7	17.774435	5
CGATTTG	180	0.0	15.999795	28
GCGATTT	190	0.0	15.999794	27
AACGCTT	200	0.0	15.199806	30
GAGTGAT	95	1.4325071E-5	15.156815	11
GTCTAGT	85	9.339839E-5	15.075369	1
AAACGCT	210	0.0	14.476006	29
CGTTATT	100	2.315641E-5	14.405709	2
TCTATGC	90	1.5305827E-4	14.225365	3
AGCGATT	225	0.0	14.22204	26
CTATTCC	225	0.0	14.220378	4
TAAACGC	215	0.0	14.139355	28
AACCGCG	125	8.5947795E-7	14.078174	7
ACCGTCG	195	2.910383E-11	13.946911	8
ATTATAC	115	5.5969413E-6	13.916118	3
GTAAACG	230	0.0	13.912865	27
GTATAGC	150	3.119567E-8	13.881903	1
TAAGAGG	300	0.0	13.864869	4
>>END_MODULE