FastQCFastQC Report
Thu 2 Feb 2017
SRR4063095_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063095_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences855845
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT34490.4029935327074412No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT24230.28311201210499565No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT14740.1722274477271001No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11180.13063113063697282No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG9390.10971612850457733No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA9320.10889822339325461No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC8790.10270551326466826No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG507.193618E-419.2001042
TCTATGC852.3911525E-718.8258323
ATAAGAC1001.3117333E-616.0019593
ATACCGT2150.015.6307336
TACCGTC2050.015.6125787
ACCGTCG1950.015.5925628
TATTACA1357.898052E-915.4074932
TCTTATA1153.341629E-715.3044322
CGTTTAT1501.9699655E-914.93254431
CGTCGTA2150.014.88380210
CTATTAG650.00416027314.7693121
CCGTCGT2200.014.5472359
TCGTTTA1654.838512E-1014.54468430
AACCGCG1351.314238E-714.2247937
GTCGTAG2400.014.00007711
ATGGTCG1503.1574018E-813.8691745
CCGATAA1503.1597665E-813.8683649
TATACTG1053.674357E-513.7167665
CTATACT1053.6763777E-513.7159634
ATTACAC1053.6763777E-513.7159633
Produced by FastQC (version 0.11.5)